Experiment: LB Anaerobic with Nitrofurantoin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ftsK and lolA are separated by 158 nucleotides lolA and ycaJ are separated by 10 nucleotides ycaJ and serS are separated by 90 nucleotides
b0890: ftsK - DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI), at 932,447 to 936,436
ftsK
b0891: lolA - chaperone for lipoproteins (RefSeq), at 936,595 to 937,206
lolA
b0892: ycaJ - recombination protein (NCBI), at 937,217 to 938,560
ycaJ
b0893: serS - seryl-tRNA synthetase (NCBI), at 938,651 to 939,943
serS
Position (kb) 937
938
939 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 936.326 kb on + strand at 936.464 kb on + strand at 936.561 kb on + strand at 937.209 kb on + strand at 937.258 kb on + strand at 937.258 kb on + strand at 937.451 kb on - strand, within ycaJ at 937.535 kb on - strand, within ycaJ at 937.629 kb on - strand, within ycaJ at 937.673 kb on + strand, within ycaJ at 937.673 kb on + strand, within ycaJ at 937.673 kb on + strand, within ycaJ at 937.694 kb on - strand, within ycaJ at 937.695 kb on + strand, within ycaJ at 937.780 kb on + strand, within ycaJ at 937.833 kb on - strand, within ycaJ at 937.985 kb on + strand, within ycaJ at 938.002 kb on + strand, within ycaJ at 938.002 kb on + strand, within ycaJ at 938.010 kb on - strand, within ycaJ at 938.024 kb on - strand, within ycaJ at 938.041 kb on + strand, within ycaJ at 938.049 kb on - strand, within ycaJ at 938.112 kb on + strand, within ycaJ at 938.120 kb on - strand, within ycaJ at 938.178 kb on - strand, within ycaJ at 938.239 kb on - strand, within ycaJ at 938.259 kb on - strand, within ycaJ at 938.415 kb on - strand, within ycaJ
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.00003125 mM remove 936,326 + +1.1 936,464 + +2.1 936,561 + +2.0 937,209 + -0.7 937,258 + +1.0 937,258 + -0.2 937,451 - ycaJ b0892 0.17 -1.0 937,535 - ycaJ b0892 0.24 +0.8 937,629 - ycaJ b0892 0.31 +0.7 937,673 + ycaJ b0892 0.34 -1.8 937,673 + ycaJ b0892 0.34 +1.9 937,673 + ycaJ b0892 0.34 +2.3 937,694 - ycaJ b0892 0.35 -0.5 937,695 + ycaJ b0892 0.36 +1.6 937,780 + ycaJ b0892 0.42 -2.5 937,833 - ycaJ b0892 0.46 +2.1 937,985 + ycaJ b0892 0.57 +0.6 938,002 + ycaJ b0892 0.58 -0.4 938,002 + ycaJ b0892 0.58 -0.3 938,010 - ycaJ b0892 0.59 -1.5 938,024 - ycaJ b0892 0.60 +4.4 938,041 + ycaJ b0892 0.61 -0.4 938,049 - ycaJ b0892 0.62 -0.6 938,112 + ycaJ b0892 0.67 +1.9 938,120 - ycaJ b0892 0.67 -0.6 938,178 - ycaJ b0892 0.72 -0.1 938,239 - ycaJ b0892 0.76 +0.9 938,259 - ycaJ b0892 0.78 -1.1 938,415 - ycaJ b0892 0.89 +0.3
Or see this region's nucleotide sequence