Experiment: LB Anaerobic with Nitrofurantoin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt allC and allD are separated by 21 nucleotides allD and fdrA are separated by 316 nucleotides
b0516: allC - N-carbamoyl-L-amino acid amidohydrolase (NCBI), at 543,281 to 544,516
allC
b0517: allD - ureidoglycolate dehydrogenase (NCBI), at 544,538 to 545,587
allD
b0518: fdrA - membrane protein FdrA (NCBI), at 545,904 to 547,571
fdrA
Position (kb) 544
545
546 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 543.642 kb on - strand, within allC at 543.642 kb on - strand, within allC at 543.642 kb on - strand, within allC at 543.670 kb on + strand, within allC at 543.736 kb on + strand, within allC at 543.750 kb on - strand, within allC at 543.772 kb on + strand, within allC at 543.812 kb on + strand, within allC at 543.813 kb on + strand, within allC at 543.813 kb on + strand, within allC at 543.813 kb on + strand, within allC at 543.819 kb on - strand, within allC at 543.819 kb on - strand, within allC at 543.827 kb on - strand, within allC at 543.839 kb on - strand, within allC at 543.845 kb on - strand, within allC at 543.867 kb on + strand, within allC at 543.867 kb on + strand, within allC at 543.870 kb on + strand, within allC at 543.928 kb on + strand, within allC at 543.933 kb on - strand, within allC at 543.933 kb on - strand, within allC at 543.936 kb on - strand, within allC at 543.936 kb on - strand, within allC at 543.936 kb on - strand, within allC at 543.938 kb on + strand, within allC at 543.938 kb on + strand, within allC at 543.938 kb on - strand, within allC at 543.948 kb on - strand, within allC at 543.974 kb on - strand, within allC at 543.974 kb on - strand, within allC at 543.995 kb on + strand, within allC at 544.025 kb on - strand, within allC at 544.064 kb on - strand, within allC at 544.064 kb on - strand, within allC at 544.081 kb on - strand, within allC at 544.118 kb on + strand, within allC at 544.125 kb on - strand, within allC at 544.164 kb on + strand, within allC at 544.167 kb on + strand, within allC at 544.167 kb on + strand, within allC at 544.187 kb on - strand, within allC at 544.187 kb on - strand, within allC at 544.217 kb on + strand, within allC at 544.217 kb on + strand, within allC at 544.248 kb on - strand, within allC at 544.269 kb on - strand, within allC at 544.274 kb on - strand, within allC at 544.281 kb on - strand, within allC at 544.283 kb on + strand, within allC at 544.291 kb on - strand, within allC at 544.296 kb on - strand, within allC at 544.296 kb on - strand, within allC at 544.297 kb on + strand, within allC at 544.297 kb on + strand, within allC at 544.314 kb on + strand, within allC at 544.335 kb on + strand, within allC at 544.353 kb on + strand, within allC at 544.353 kb on + strand, within allC at 544.357 kb on + strand, within allC at 544.396 kb on + strand at 544.396 kb on + strand at 544.424 kb on + strand at 544.433 kb on + strand at 544.436 kb on + strand at 544.444 kb on + strand at 544.449 kb on + strand at 544.451 kb on - strand at 544.453 kb on - strand at 544.455 kb on + strand at 544.455 kb on + strand at 544.456 kb on + strand at 544.464 kb on + strand at 544.484 kb on + strand at 544.484 kb on + strand at 544.507 kb on + strand at 544.523 kb on + strand at 544.574 kb on + strand at 544.638 kb on - strand at 544.658 kb on + strand, within allD at 544.714 kb on - strand, within allD at 544.733 kb on - strand, within allD at 544.733 kb on - strand, within allD at 544.733 kb on - strand, within allD at 544.739 kb on - strand, within allD at 544.799 kb on - strand, within allD at 544.828 kb on - strand, within allD at 544.828 kb on - strand, within allD at 544.842 kb on - strand, within allD at 544.873 kb on - strand, within allD at 544.877 kb on - strand, within allD at 544.878 kb on + strand, within allD at 544.901 kb on - strand, within allD at 544.901 kb on - strand, within allD at 544.914 kb on - strand, within allD at 544.937 kb on - strand, within allD at 544.939 kb on + strand, within allD at 544.940 kb on + strand, within allD at 544.947 kb on + strand, within allD at 544.950 kb on - strand, within allD at 544.982 kb on - strand, within allD at 544.997 kb on - strand, within allD at 544.997 kb on - strand, within allD at 545.028 kb on - strand, within allD at 545.045 kb on + strand, within allD at 545.144 kb on + strand, within allD at 545.203 kb on + strand, within allD at 545.218 kb on - strand, within allD at 545.245 kb on - strand, within allD at 545.312 kb on - strand, within allD at 545.327 kb on + strand, within allD at 545.395 kb on - strand, within allD at 545.430 kb on + strand, within allD at 545.430 kb on + strand, within allD at 545.469 kb on + strand, within allD at 545.481 kb on + strand, within allD at 545.481 kb on + strand, within allD at 545.499 kb on + strand at 545.505 kb on + strand at 545.505 kb on + strand at 545.505 kb on + strand at 545.505 kb on + strand at 545.513 kb on + strand at 545.561 kb on + strand at 545.568 kb on + strand at 545.606 kb on + strand at 545.609 kb on + strand at 545.611 kb on + strand at 545.611 kb on + strand at 545.853 kb on + strand at 545.853 kb on + strand at 545.853 kb on + strand at 545.856 kb on - strand at 545.856 kb on - strand at 545.861 kb on - strand at 545.864 kb on - strand at 545.888 kb on + strand at 545.888 kb on + strand at 546.052 kb on - strand at 546.152 kb on - strand, within fdrA at 546.152 kb on - strand, within fdrA at 546.166 kb on + strand, within fdrA at 546.166 kb on + strand, within fdrA at 546.221 kb on + strand, within fdrA at 546.325 kb on + strand, within fdrA at 546.411 kb on + strand, within fdrA at 546.427 kb on + strand, within fdrA at 546.559 kb on + strand, within fdrA at 546.559 kb on + strand, within fdrA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.00003125 mM remove 543,642 - allC b0516 0.29 +0.9 543,642 - allC b0516 0.29 -0.2 543,642 - allC b0516 0.29 +0.1 543,670 + allC b0516 0.31 -1.9 543,736 + allC b0516 0.37 -0.3 543,750 - allC b0516 0.38 -0.9 543,772 + allC b0516 0.40 -0.7 543,812 + allC b0516 0.43 -2.4 543,813 + allC b0516 0.43 +2.3 543,813 + allC b0516 0.43 -0.5 543,813 + allC b0516 0.43 -1.9 543,819 - allC b0516 0.44 -0.0 543,819 - allC b0516 0.44 +0.2 543,827 - allC b0516 0.44 +1.0 543,839 - allC b0516 0.45 +1.1 543,845 - allC b0516 0.46 +1.7 543,867 + allC b0516 0.47 -1.5 543,867 + allC b0516 0.47 -1.3 543,870 + allC b0516 0.48 -0.3 543,928 + allC b0516 0.52 +0.3 543,933 - allC b0516 0.53 -0.2 543,933 - allC b0516 0.53 -1.4 543,936 - allC b0516 0.53 +0.4 543,936 - allC b0516 0.53 +3.0 543,936 - allC b0516 0.53 +0.1 543,938 + allC b0516 0.53 -0.3 543,938 + allC b0516 0.53 -0.1 543,938 - allC b0516 0.53 +0.5 543,948 - allC b0516 0.54 -1.6 543,974 - allC b0516 0.56 +1.4 543,974 - allC b0516 0.56 +0.4 543,995 + allC b0516 0.58 +0.3 544,025 - allC b0516 0.60 +1.0 544,064 - allC b0516 0.63 -0.9 544,064 - allC b0516 0.63 -2.0 544,081 - allC b0516 0.65 +0.2 544,118 + allC b0516 0.68 +1.3 544,125 - allC b0516 0.68 -1.3 544,164 + allC b0516 0.71 +0.3 544,167 + allC b0516 0.72 +1.7 544,167 + allC b0516 0.72 +0.3 544,187 - allC b0516 0.73 -3.4 544,187 - allC b0516 0.73 -0.0 544,217 + allC b0516 0.76 +1.3 544,217 + allC b0516 0.76 -0.3 544,248 - allC b0516 0.78 +0.2 544,269 - allC b0516 0.80 +2.8 544,274 - allC b0516 0.80 +0.2 544,281 - allC b0516 0.81 -0.3 544,283 + allC b0516 0.81 -0.3 544,291 - allC b0516 0.82 -1.9 544,296 - allC b0516 0.82 +4.4 544,296 - allC b0516 0.82 -1.8 544,297 + allC b0516 0.82 -1.3 544,297 + allC b0516 0.82 +1.7 544,314 + allC b0516 0.84 +1.3 544,335 + allC b0516 0.85 -1.7 544,353 + allC b0516 0.87 -0.3 544,353 + allC b0516 0.87 +3.0 544,357 + allC b0516 0.87 +0.3 544,396 + -0.7 544,396 + -1.0 544,424 + +2.1 544,433 + -0.7 544,436 + +1.3 544,444 + +1.3 544,449 + +1.3 544,451 - -0.3 544,453 - +1.3 544,455 + +0.3 544,455 + +1.3 544,456 + -0.3 544,464 + -1.9 544,484 + +0.2 544,484 + +0.3 544,507 + -2.4 544,523 + +1.0 544,574 + +1.3 544,638 - +1.3 544,658 + allD b0517 0.11 +1.3 544,714 - allD b0517 0.17 +1.3 544,733 - allD b0517 0.19 +0.0 544,733 - allD b0517 0.19 -0.0 544,733 - allD b0517 0.19 -0.8 544,739 - allD b0517 0.19 +1.3 544,799 - allD b0517 0.25 -1.7 544,828 - allD b0517 0.28 +0.3 544,828 - allD b0517 0.28 -1.5 544,842 - allD b0517 0.29 -1.3 544,873 - allD b0517 0.32 -1.3 544,877 - allD b0517 0.32 +0.2 544,878 + allD b0517 0.32 -2.2 544,901 - allD b0517 0.35 +0.1 544,901 - allD b0517 0.35 +0.5 544,914 - allD b0517 0.36 +1.9 544,937 - allD b0517 0.38 -0.4 544,939 + allD b0517 0.38 +0.1 544,940 + allD b0517 0.38 -0.5 544,947 + allD b0517 0.39 +0.5 544,950 - allD b0517 0.39 -1.2 544,982 - allD b0517 0.42 +0.0 544,997 - allD b0517 0.44 -1.8 544,997 - allD b0517 0.44 -0.3 545,028 - allD b0517 0.47 +0.3 545,045 + allD b0517 0.48 +0.6 545,144 + allD b0517 0.58 -2.0 545,203 + allD b0517 0.63 +1.2 545,218 - allD b0517 0.65 -1.0 545,245 - allD b0517 0.67 -0.2 545,312 - allD b0517 0.74 -1.7 545,327 + allD b0517 0.75 -1.4 545,395 - allD b0517 0.82 -2.6 545,430 + allD b0517 0.85 +0.7 545,430 + allD b0517 0.85 -1.0 545,469 + allD b0517 0.89 -0.7 545,481 + allD b0517 0.90 -0.7 545,481 + allD b0517 0.90 +0.9 545,499 + +0.7 545,505 + -1.1 545,505 + -0.3 545,505 + +0.7 545,505 + +0.1 545,513 + -1.0 545,561 + -0.3 545,568 + -1.9 545,606 + +6.1 545,609 + +0.3 545,611 + +0.3 545,611 + +1.6 545,853 + +1.3 545,853 + +1.3 545,853 + +1.3 545,856 - +1.3 545,856 - +1.3 545,861 - -0.3 545,864 - +1.3 545,888 + -0.3 545,888 + +1.3 546,052 - +0.3 546,152 - fdrA b0518 0.15 -2.4 546,152 - fdrA b0518 0.15 -0.7 546,166 + fdrA b0518 0.16 +2.1 546,166 + fdrA b0518 0.16 -1.7 546,221 + fdrA b0518 0.19 +1.7 546,325 + fdrA b0518 0.25 +0.8 546,411 + fdrA b0518 0.30 -0.5 546,427 + fdrA b0518 0.31 +0.5 546,559 + fdrA b0518 0.39 +0.0 546,559 + fdrA b0518 0.39 -1.7
Or see this region's nucleotide sequence