Experiment: LB Anaerobic with Nitrofurantoin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glxR and ybbW are separated by 168 nucleotides ybbW and allB are separated by 59 nucleotides
b0509: glxR - tartronate semialdehyde reductase, NADH-dependent (NCBI), at 535,810 to 536,688
glxR
b0511: ybbW - putative transport protein (VIMSS), at 536,857 to 538,311
ybbW
b0512: allB - allantoinase (NCBI), at 538,371 to 539,732
allB
Position (kb) 536
537
538
539 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 535.860 kb on - strand at 535.882 kb on + strand at 535.890 kb on - strand at 535.892 kb on - strand at 535.952 kb on - strand, within glxR at 535.967 kb on - strand, within glxR at 535.969 kb on - strand, within glxR at 535.998 kb on + strand, within glxR at 536.006 kb on + strand, within glxR at 536.007 kb on + strand, within glxR at 536.007 kb on + strand, within glxR at 536.048 kb on - strand, within glxR at 536.128 kb on + strand, within glxR at 536.174 kb on + strand, within glxR at 536.187 kb on + strand, within glxR at 536.195 kb on - strand, within glxR at 536.295 kb on - strand, within glxR at 536.308 kb on - strand, within glxR at 536.393 kb on - strand, within glxR at 536.429 kb on + strand, within glxR at 536.492 kb on - strand, within glxR at 536.522 kb on + strand, within glxR at 536.533 kb on + strand, within glxR at 536.533 kb on + strand, within glxR at 536.536 kb on - strand, within glxR at 536.595 kb on + strand, within glxR at 536.595 kb on + strand, within glxR at 536.626 kb on + strand at 536.626 kb on + strand at 536.631 kb on + strand at 536.636 kb on + strand at 536.637 kb on - strand at 536.639 kb on + strand at 536.641 kb on + strand at 536.641 kb on + strand at 536.641 kb on + strand at 536.641 kb on + strand at 536.658 kb on + strand at 536.658 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.672 kb on + strand at 536.673 kb on + strand at 536.982 kb on - strand at 536.984 kb on - strand at 537.041 kb on - strand, within ybbW at 537.096 kb on - strand, within ybbW at 537.096 kb on - strand, within ybbW at 537.106 kb on - strand, within ybbW at 537.116 kb on - strand, within ybbW at 537.119 kb on - strand, within ybbW at 537.119 kb on - strand, within ybbW at 537.119 kb on - strand, within ybbW at 537.121 kb on - strand, within ybbW at 537.146 kb on + strand, within ybbW at 537.152 kb on + strand, within ybbW at 537.155 kb on + strand, within ybbW at 537.155 kb on + strand, within ybbW at 537.155 kb on + strand, within ybbW at 537.155 kb on - strand, within ybbW at 537.155 kb on - strand, within ybbW at 537.157 kb on + strand, within ybbW at 537.163 kb on - strand, within ybbW at 537.163 kb on - strand, within ybbW at 537.171 kb on + strand, within ybbW at 537.207 kb on + strand, within ybbW at 537.215 kb on - strand, within ybbW at 537.215 kb on - strand, within ybbW at 537.225 kb on + strand, within ybbW at 537.225 kb on + strand, within ybbW at 537.227 kb on + strand, within ybbW at 537.227 kb on + strand, within ybbW at 537.235 kb on - strand, within ybbW at 537.235 kb on - strand, within ybbW at 537.237 kb on + strand, within ybbW at 537.237 kb on + strand, within ybbW at 537.238 kb on + strand, within ybbW at 537.238 kb on + strand, within ybbW at 537.238 kb on + strand, within ybbW at 537.238 kb on + strand, within ybbW at 537.238 kb on + strand, within ybbW at 537.239 kb on - strand, within ybbW at 537.246 kb on - strand, within ybbW at 537.246 kb on - strand, within ybbW at 537.248 kb on + strand, within ybbW at 537.248 kb on + strand, within ybbW at 537.256 kb on - strand, within ybbW at 537.256 kb on - strand, within ybbW at 537.299 kb on + strand, within ybbW at 537.307 kb on + strand, within ybbW at 537.307 kb on + strand, within ybbW at 537.309 kb on + strand, within ybbW at 537.315 kb on - strand, within ybbW at 537.327 kb on - strand, within ybbW at 537.362 kb on + strand, within ybbW at 537.362 kb on + strand, within ybbW at 537.367 kb on + strand, within ybbW at 537.368 kb on + strand, within ybbW at 537.368 kb on + strand, within ybbW at 537.421 kb on - strand, within ybbW at 537.423 kb on - strand, within ybbW at 537.423 kb on - strand, within ybbW at 537.437 kb on - strand, within ybbW at 537.437 kb on - strand, within ybbW at 537.479 kb on - strand, within ybbW at 537.479 kb on - strand, within ybbW at 537.479 kb on - strand, within ybbW at 537.479 kb on - strand, within ybbW at 537.479 kb on - strand, within ybbW at 537.517 kb on - strand, within ybbW at 537.551 kb on + strand, within ybbW at 537.551 kb on + strand, within ybbW at 537.552 kb on + strand, within ybbW at 537.565 kb on + strand, within ybbW at 537.583 kb on - strand, within ybbW at 537.583 kb on - strand, within ybbW at 537.644 kb on + strand, within ybbW at 537.644 kb on + strand, within ybbW at 537.659 kb on + strand, within ybbW at 537.669 kb on - strand, within ybbW at 537.669 kb on - strand, within ybbW at 537.676 kb on + strand, within ybbW at 537.678 kb on + strand, within ybbW at 537.698 kb on + strand, within ybbW at 537.702 kb on - strand, within ybbW at 537.716 kb on + strand, within ybbW at 537.718 kb on + strand, within ybbW at 537.718 kb on + strand, within ybbW at 537.719 kb on - strand, within ybbW at 537.724 kb on - strand, within ybbW at 537.726 kb on - strand, within ybbW at 537.726 kb on - strand, within ybbW at 537.730 kb on + strand, within ybbW at 537.750 kb on + strand, within ybbW at 537.750 kb on + strand, within ybbW at 537.757 kb on + strand, within ybbW at 537.757 kb on + strand, within ybbW at 537.762 kb on + strand, within ybbW at 537.762 kb on + strand, within ybbW at 537.762 kb on + strand, within ybbW at 537.762 kb on + strand, within ybbW at 537.767 kb on + strand, within ybbW at 537.773 kb on + strand, within ybbW at 537.773 kb on + strand, within ybbW at 537.773 kb on + strand, within ybbW at 537.773 kb on + strand, within ybbW at 537.774 kb on + strand, within ybbW at 537.775 kb on - strand, within ybbW at 537.791 kb on + strand, within ybbW at 537.791 kb on + strand, within ybbW at 537.810 kb on + strand, within ybbW at 537.820 kb on + strand, within ybbW at 537.822 kb on + strand, within ybbW at 537.827 kb on - strand, within ybbW at 537.827 kb on - strand, within ybbW at 537.857 kb on + strand, within ybbW at 537.857 kb on + strand, within ybbW at 537.907 kb on + strand, within ybbW at 537.922 kb on + strand, within ybbW at 537.929 kb on + strand, within ybbW at 538.060 kb on + strand, within ybbW at 538.134 kb on + strand, within ybbW at 538.190 kb on + strand at 538.190 kb on + strand at 538.246 kb on + strand at 538.253 kb on + strand at 538.266 kb on + strand at 538.266 kb on + strand at 538.269 kb on + strand at 538.269 kb on + strand at 538.291 kb on + strand at 538.313 kb on - strand at 538.370 kb on - strand at 538.437 kb on - strand at 538.484 kb on - strand at 538.484 kb on - strand at 538.494 kb on - strand at 538.494 kb on - strand at 538.507 kb on + strand at 538.515 kb on - strand, within allB at 538.515 kb on - strand, within allB at 538.515 kb on - strand, within allB at 538.567 kb on + strand, within allB at 538.593 kb on - strand, within allB at 538.660 kb on - strand, within allB at 538.736 kb on + strand, within allB at 538.745 kb on + strand, within allB at 538.752 kb on - strand, within allB at 538.752 kb on - strand, within allB at 538.815 kb on + strand, within allB at 538.828 kb on - strand, within allB at 538.920 kb on + strand, within allB at 538.925 kb on - strand, within allB at 539.002 kb on + strand, within allB at 539.002 kb on + strand, within allB at 539.008 kb on - strand, within allB at 539.112 kb on + strand, within allB at 539.145 kb on + strand, within allB at 539.145 kb on + strand, within allB at 539.145 kb on + strand, within allB at 539.180 kb on + strand, within allB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.00003125 mM remove 535,860 - -0.3 535,882 + -0.3 535,890 - -0.3 535,892 - -0.7 535,952 - glxR b0509 0.16 +1.3 535,967 - glxR b0509 0.18 +0.3 535,969 - glxR b0509 0.18 -1.0 535,998 + glxR b0509 0.21 +0.9 536,006 + glxR b0509 0.22 +0.3 536,007 + glxR b0509 0.22 -0.2 536,007 + glxR b0509 0.22 -0.6 536,048 - glxR b0509 0.27 +0.1 536,128 + glxR b0509 0.36 +0.2 536,174 + glxR b0509 0.41 -0.2 536,187 + glxR b0509 0.43 +1.5 536,195 - glxR b0509 0.44 +1.3 536,295 - glxR b0509 0.55 -0.7 536,308 - glxR b0509 0.57 -0.3 536,393 - glxR b0509 0.66 +0.3 536,429 + glxR b0509 0.70 +0.9 536,492 - glxR b0509 0.78 +1.3 536,522 + glxR b0509 0.81 +1.2 536,533 + glxR b0509 0.82 +2.1 536,533 + glxR b0509 0.82 -0.7 536,536 - glxR b0509 0.83 +1.3 536,595 + glxR b0509 0.89 -1.6 536,595 + glxR b0509 0.89 +0.4 536,626 + +0.9 536,626 + -0.4 536,631 + -3.0 536,636 + +0.6 536,637 - +1.3 536,639 + -0.3 536,641 + -2.7 536,641 + -1.5 536,641 + +1.1 536,641 + +1.9 536,658 + -1.9 536,658 + -0.7 536,672 + -2.8 536,672 + -0.3 536,672 + +0.7 536,672 + -0.2 536,672 + +1.7 536,672 + -1.7 536,672 + -1.3 536,672 + -0.4 536,672 + -1.9 536,672 + -1.7 536,672 + -2.4 536,672 + -0.4 536,672 + +0.5 536,672 + +0.1 536,673 + +0.6 536,982 - +5.9 536,984 - +1.3 537,041 - ybbW b0511 0.13 +0.3 537,096 - ybbW b0511 0.16 +0.3 537,096 - ybbW b0511 0.16 +1.3 537,106 - ybbW b0511 0.17 -1.0 537,116 - ybbW b0511 0.18 +1.0 537,119 - ybbW b0511 0.18 +1.3 537,119 - ybbW b0511 0.18 -1.8 537,119 - ybbW b0511 0.18 +1.3 537,121 - ybbW b0511 0.18 -0.7 537,146 + ybbW b0511 0.20 +1.3 537,152 + ybbW b0511 0.20 +1.3 537,155 + ybbW b0511 0.20 -0.1 537,155 + ybbW b0511 0.20 +1.3 537,155 + ybbW b0511 0.20 -1.3 537,155 - ybbW b0511 0.20 +1.3 537,155 - ybbW b0511 0.20 +0.3 537,157 + ybbW b0511 0.21 +1.3 537,163 - ybbW b0511 0.21 +2.0 537,163 - ybbW b0511 0.21 +1.3 537,171 + ybbW b0511 0.22 +1.3 537,207 + ybbW b0511 0.24 -0.7 537,215 - ybbW b0511 0.25 +1.3 537,215 - ybbW b0511 0.25 +0.3 537,225 + ybbW b0511 0.25 +0.3 537,225 + ybbW b0511 0.25 -0.3 537,227 + ybbW b0511 0.25 +0.3 537,227 + ybbW b0511 0.25 +0.3 537,235 - ybbW b0511 0.26 +1.3 537,235 - ybbW b0511 0.26 +1.3 537,237 + ybbW b0511 0.26 -0.3 537,237 + ybbW b0511 0.26 +0.3 537,238 + ybbW b0511 0.26 +0.3 537,238 + ybbW b0511 0.26 -1.0 537,238 + ybbW b0511 0.26 -0.3 537,238 + ybbW b0511 0.26 +0.3 537,238 + ybbW b0511 0.26 +0.3 537,239 - ybbW b0511 0.26 +1.3 537,246 - ybbW b0511 0.27 +1.3 537,246 - ybbW b0511 0.27 +1.3 537,248 + ybbW b0511 0.27 -0.3 537,248 + ybbW b0511 0.27 -0.7 537,256 - ybbW b0511 0.27 +0.3 537,256 - ybbW b0511 0.27 +1.3 537,299 + ybbW b0511 0.30 -1.5 537,307 + ybbW b0511 0.31 +0.3 537,307 + ybbW b0511 0.31 -0.0 537,309 + ybbW b0511 0.31 +0.3 537,315 - ybbW b0511 0.31 +1.3 537,327 - ybbW b0511 0.32 +1.3 537,362 + ybbW b0511 0.35 +1.3 537,362 + ybbW b0511 0.35 -0.3 537,367 + ybbW b0511 0.35 -1.5 537,368 + ybbW b0511 0.35 -0.7 537,368 + ybbW b0511 0.35 +0.3 537,421 - ybbW b0511 0.39 -1.0 537,423 - ybbW b0511 0.39 -0.3 537,423 - ybbW b0511 0.39 +1.3 537,437 - ybbW b0511 0.40 +1.3 537,437 - ybbW b0511 0.40 +1.3 537,479 - ybbW b0511 0.43 +0.3 537,479 - ybbW b0511 0.43 +1.3 537,479 - ybbW b0511 0.43 -1.7 537,479 - ybbW b0511 0.43 -0.3 537,479 - ybbW b0511 0.43 +1.3 537,517 - ybbW b0511 0.45 +1.3 537,551 + ybbW b0511 0.48 +0.3 537,551 + ybbW b0511 0.48 -0.3 537,552 + ybbW b0511 0.48 -0.5 537,565 + ybbW b0511 0.49 -0.1 537,583 - ybbW b0511 0.50 +1.3 537,583 - ybbW b0511 0.50 +1.3 537,644 + ybbW b0511 0.54 -2.7 537,644 + ybbW b0511 0.54 -0.3 537,659 + ybbW b0511 0.55 -0.0 537,669 - ybbW b0511 0.56 -0.7 537,669 - ybbW b0511 0.56 +1.3 537,676 + ybbW b0511 0.56 +0.1 537,678 + ybbW b0511 0.56 -2.3 537,698 + ybbW b0511 0.58 +0.3 537,702 - ybbW b0511 0.58 +0.4 537,716 + ybbW b0511 0.59 -1.0 537,718 + ybbW b0511 0.59 -1.0 537,718 + ybbW b0511 0.59 -0.5 537,719 - ybbW b0511 0.59 -0.1 537,724 - ybbW b0511 0.60 +0.7 537,726 - ybbW b0511 0.60 -0.0 537,726 - ybbW b0511 0.60 +0.3 537,730 + ybbW b0511 0.60 -0.7 537,750 + ybbW b0511 0.61 -0.6 537,750 + ybbW b0511 0.61 -0.3 537,757 + ybbW b0511 0.62 -1.3 537,757 + ybbW b0511 0.62 -0.7 537,762 + ybbW b0511 0.62 +2.5 537,762 + ybbW b0511 0.62 +0.5 537,762 + ybbW b0511 0.62 +2.3 537,762 + ybbW b0511 0.62 -1.5 537,767 + ybbW b0511 0.63 -1.3 537,773 + ybbW b0511 0.63 -1.5 537,773 + ybbW b0511 0.63 +4.6 537,773 + ybbW b0511 0.63 -1.7 537,773 + ybbW b0511 0.63 +0.5 537,774 + ybbW b0511 0.63 -1.4 537,775 - ybbW b0511 0.63 +0.3 537,791 + ybbW b0511 0.64 -0.5 537,791 + ybbW b0511 0.64 -1.7 537,810 + ybbW b0511 0.65 +1.3 537,820 + ybbW b0511 0.66 +0.3 537,822 + ybbW b0511 0.66 -2.0 537,827 - ybbW b0511 0.67 -1.0 537,827 - ybbW b0511 0.67 +1.3 537,857 + ybbW b0511 0.69 +0.3 537,857 + ybbW b0511 0.69 +0.3 537,907 + ybbW b0511 0.72 -0.3 537,922 + ybbW b0511 0.73 +0.7 537,929 + ybbW b0511 0.74 -0.9 538,060 + ybbW b0511 0.83 +1.3 538,134 + ybbW b0511 0.88 +1.3 538,190 + +1.3 538,190 + +1.3 538,246 + -1.7 538,253 + -1.3 538,266 + +1.3 538,266 + -1.0 538,269 + +0.3 538,269 + -1.5 538,291 + -0.1 538,313 - +1.3 538,370 - -0.3 538,437 - -2.0 538,484 - +1.3 538,484 - +0.3 538,494 - -0.3 538,494 - +2.3 538,507 + +0.3 538,515 - allB b0512 0.11 -0.7 538,515 - allB b0512 0.11 +0.3 538,515 - allB b0512 0.11 +1.7 538,567 + allB b0512 0.14 -0.1 538,593 - allB b0512 0.16 -1.0 538,660 - allB b0512 0.21 +1.0 538,736 + allB b0512 0.27 +0.3 538,745 + allB b0512 0.27 -1.0 538,752 - allB b0512 0.28 +0.2 538,752 - allB b0512 0.28 -0.5 538,815 + allB b0512 0.33 -1.2 538,828 - allB b0512 0.34 -1.0 538,920 + allB b0512 0.40 -0.3 538,925 - allB b0512 0.41 +1.5 539,002 + allB b0512 0.46 -2.5 539,002 + allB b0512 0.46 +0.1 539,008 - allB b0512 0.47 +0.9 539,112 + allB b0512 0.54 -1.7 539,145 + allB b0512 0.57 +0.6 539,145 + allB b0512 0.57 -0.0 539,145 + allB b0512 0.57 -1.5 539,180 + allB b0512 0.59 +0.8
Or see this region's nucleotide sequence