Experiment: LB Anaerobic with Nitrofurantoin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafY and ykfL overlap by 1 nucleotides ykfL and ykfK are separated by 1 nucleotides ykfK and yafZ are separated by 216 nucleotides yafZ and ykfA are separated by 91 nucleotides
b0251: yafY - CP4-6 prophage; predicted DNA-binding transcriptional regulator (RefSeq), at 265,334 to 265,777
yafY
b4627: ykfL - no description, at 265,777 to 265,998
ykfL
b4628: ykfK - no description, at 266,000 to 266,191
ykfK
b0252: yafZ - orf, hypothetical protein (VIMSS), at 266,408 to 267,229
yafZ
b0253: ykfA - putative GTP-binding protein (VIMSS), at 267,321 to 268,184
ykfA
Position (kb) 266
267
268 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 265.426 kb on - strand, within yafY at 265.436 kb on - strand, within yafY at 265.519 kb on - strand, within yafY at 265.903 kb on + strand, within ykfL at 265.906 kb on - strand, within ykfL at 265.943 kb on - strand, within ykfL at 265.943 kb on - strand, within ykfL at 265.945 kb on + strand, within ykfL at 265.945 kb on + strand, within ykfL at 265.951 kb on - strand, within ykfL at 266.018 kb on + strand at 266.018 kb on + strand at 266.187 kb on + strand at 266.217 kb on - strand at 266.258 kb on + strand at 266.267 kb on - strand at 266.267 kb on - strand at 266.267 kb on - strand at 266.353 kb on - strand at 266.433 kb on + strand at 266.436 kb on - strand at 266.449 kb on + strand at 266.464 kb on - strand at 266.569 kb on - strand, within yafZ at 266.668 kb on - strand, within yafZ at 266.773 kb on - strand, within yafZ at 266.810 kb on - strand, within yafZ at 266.946 kb on - strand, within yafZ at 267.028 kb on - strand, within yafZ at 267.028 kb on - strand, within yafZ at 267.062 kb on - strand, within yafZ at 267.062 kb on - strand, within yafZ at 267.093 kb on + strand, within yafZ at 267.122 kb on + strand, within yafZ at 267.137 kb on + strand, within yafZ at 267.137 kb on + strand, within yafZ at 267.168 kb on - strand at 267.229 kb on + strand at 267.229 kb on + strand at 267.289 kb on + strand at 267.289 kb on + strand at 267.317 kb on + strand at 267.320 kb on - strand at 267.351 kb on - strand at 267.351 kb on - strand at 267.437 kb on - strand, within ykfA at 267.580 kb on - strand, within ykfA at 267.604 kb on + strand, within ykfA at 267.607 kb on - strand, within ykfA at 267.607 kb on - strand, within ykfA at 267.617 kb on - strand, within ykfA at 267.622 kb on + strand, within ykfA at 267.622 kb on + strand, within ykfA at 267.622 kb on + strand, within ykfA at 267.687 kb on - strand, within ykfA at 267.715 kb on - strand, within ykfA at 267.716 kb on + strand, within ykfA at 267.720 kb on - strand, within ykfA at 267.726 kb on + strand, within ykfA at 267.734 kb on - strand, within ykfA at 267.758 kb on - strand, within ykfA at 267.771 kb on - strand, within ykfA at 267.828 kb on + strand, within ykfA at 267.838 kb on - strand, within ykfA at 267.838 kb on - strand, within ykfA at 267.864 kb on - strand, within ykfA at 267.869 kb on - strand, within ykfA at 267.879 kb on - strand, within ykfA at 267.879 kb on - strand, within ykfA at 267.879 kb on - strand, within ykfA at 267.879 kb on - strand, within ykfA at 267.909 kb on + strand, within ykfA at 267.933 kb on - strand, within ykfA at 267.978 kb on - strand, within ykfA at 267.978 kb on - strand, within ykfA at 268.000 kb on + strand, within ykfA at 268.120 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.00003125 mM remove 265,426 - yafY b0251 0.21 -1.5 265,436 - yafY b0251 0.23 +1.1 265,519 - yafY b0251 0.42 -0.5 265,903 + ykfL b4627 0.57 +0.6 265,906 - ykfL b4627 0.58 -1.1 265,943 - ykfL b4627 0.75 +3.1 265,943 - ykfL b4627 0.75 -2.5 265,945 + ykfL b4627 0.76 -0.9 265,945 + ykfL b4627 0.76 +0.6 265,951 - ykfL b4627 0.78 -0.5 266,018 + +0.6 266,018 + +1.0 266,187 + -1.9 266,217 - -0.5 266,258 + +1.3 266,267 - -1.6 266,267 - +0.6 266,267 - -2.4 266,353 - -0.7 266,433 + +3.2 266,436 - +2.6 266,449 + -3.0 266,464 - -1.3 266,569 - yafZ b0252 0.20 +1.6 266,668 - yafZ b0252 0.32 +0.4 266,773 - yafZ b0252 0.44 +2.3 266,810 - yafZ b0252 0.49 -0.2 266,946 - yafZ b0252 0.65 +0.1 267,028 - yafZ b0252 0.75 +1.3 267,028 - yafZ b0252 0.75 +1.3 267,062 - yafZ b0252 0.80 +0.6 267,062 - yafZ b0252 0.80 +0.1 267,093 + yafZ b0252 0.83 +0.1 267,122 + yafZ b0252 0.87 -2.8 267,137 + yafZ b0252 0.89 +0.2 267,137 + yafZ b0252 0.89 +0.6 267,168 - -1.6 267,229 + +1.6 267,229 + -0.1 267,289 + +0.2 267,289 + +0.4 267,317 + +0.1 267,320 - +0.2 267,351 - +2.4 267,351 - -2.2 267,437 - ykfA b0253 0.13 +0.7 267,580 - ykfA b0253 0.30 -0.3 267,604 + ykfA b0253 0.33 -0.4 267,607 - ykfA b0253 0.33 +1.9 267,607 - ykfA b0253 0.33 +0.0 267,617 - ykfA b0253 0.34 +1.2 267,622 + ykfA b0253 0.35 -2.1 267,622 + ykfA b0253 0.35 -2.0 267,622 + ykfA b0253 0.35 -0.4 267,687 - ykfA b0253 0.42 -0.5 267,715 - ykfA b0253 0.46 +1.0 267,716 + ykfA b0253 0.46 -0.7 267,720 - ykfA b0253 0.46 +0.2 267,726 + ykfA b0253 0.47 +1.4 267,734 - ykfA b0253 0.48 -0.4 267,758 - ykfA b0253 0.51 -2.1 267,771 - ykfA b0253 0.52 -0.0 267,828 + ykfA b0253 0.59 -0.3 267,838 - ykfA b0253 0.60 -0.0 267,838 - ykfA b0253 0.60 -0.6 267,864 - ykfA b0253 0.63 +0.6 267,869 - ykfA b0253 0.63 +1.4 267,879 - ykfA b0253 0.65 +0.5 267,879 - ykfA b0253 0.65 -0.6 267,879 - ykfA b0253 0.65 -1.2 267,879 - ykfA b0253 0.65 -2.0 267,909 + ykfA b0253 0.68 +0.5 267,933 - ykfA b0253 0.71 +0.4 267,978 - ykfA b0253 0.76 +2.0 267,978 - ykfA b0253 0.76 +0.8 268,000 + ykfA b0253 0.79 +1.7 268,120 + +0.1
Or see this region's nucleotide sequence