Strain Fitness in Escherichia coli BW25113 around b0106

Experiment: LB Anaerobic with Nitrofurantoin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntguaC and hofC are separated by 34 nucleotideshofC and hofB overlap by 11 nucleotides b0104: guaC - guanosine 5'-monophosphate oxidoreductase (NCBI), at 113,444 to 114,487 guaC b0106: hofC - assembly protein in type IV pilin biogenesis, transmembrane protein (NCBI), at 114,522 to 115,724 hofC b0107: hofB - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 115,714 to 117,099 hofB Position (kb) 114 115 116Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 113.564 kb on + strand, within guaCat 113.668 kb on + strand, within guaCat 113.668 kb on + strand, within guaCat 113.788 kb on + strand, within guaCat 113.788 kb on + strand, within guaCat 113.803 kb on + strand, within guaCat 113.803 kb on + strand, within guaCat 113.854 kb on + strand, within guaCat 114.020 kb on + strand, within guaCat 114.039 kb on + strand, within guaCat 114.075 kb on + strand, within guaCat 114.075 kb on + strand, within guaCat 114.082 kb on + strand, within guaCat 114.082 kb on + strand, within guaCat 114.121 kb on - strand, within guaCat 114.121 kb on - strand, within guaCat 114.191 kb on - strand, within guaCat 114.191 kb on - strand, within guaCat 114.217 kb on - strand, within guaCat 114.236 kb on - strand, within guaCat 114.246 kb on - strand, within guaCat 114.278 kb on - strand, within guaCat 114.310 kb on + strand, within guaCat 114.354 kb on - strand, within guaCat 114.399 kb on - strandat 114.422 kb on - strandat 114.480 kb on + strandat 114.480 kb on + strandat 114.509 kb on - strandat 114.509 kb on - strandat 114.519 kb on + strandat 114.524 kb on + strandat 114.527 kb on - strandat 114.527 kb on - strandat 114.532 kb on - strandat 114.535 kb on - strandat 114.545 kb on - strandat 114.562 kb on + strandat 114.627 kb on + strandat 114.634 kb on + strandat 114.680 kb on + strand, within hofCat 114.838 kb on + strand, within hofCat 114.838 kb on + strand, within hofCat 114.844 kb on + strand, within hofCat 114.928 kb on - strand, within hofCat 114.930 kb on - strandat 114.930 kb on - strand, within hofCat 114.998 kb on + strand, within hofCat 114.998 kb on + strand, within hofCat 115.010 kb on - strand, within hofCat 115.021 kb on + strand, within hofCat 115.021 kb on + strand, within hofCat 115.077 kb on - strand, within hofCat 115.152 kb on - strand, within hofCat 115.152 kb on - strand, within hofCat 115.201 kb on - strand, within hofCat 115.203 kb on - strand, within hofCat 115.203 kb on - strand, within hofCat 115.273 kb on + strand, within hofCat 115.278 kb on + strand, within hofCat 115.278 kb on + strand, within hofCat 115.330 kb on + strand, within hofCat 115.367 kb on + strand, within hofCat 115.367 kb on + strand, within hofCat 115.379 kb on + strand, within hofCat 115.379 kb on + strand, within hofCat 115.409 kb on + strand, within hofCat 115.474 kb on - strand, within hofCat 115.507 kb on + strand, within hofCat 115.507 kb on + strand, within hofCat 115.515 kb on - strand, within hofCat 115.624 kb on + strandat 115.651 kb on + strandat 115.699 kb on - strandat 115.737 kb on + strandat 115.856 kb on - strand, within hofBat 115.856 kb on - strand, within hofBat 115.910 kb on + strand, within hofBat 115.911 kb on - strand, within hofBat 115.911 kb on - strand, within hofBat 115.941 kb on + strand, within hofBat 115.980 kb on + strand, within hofBat 116.058 kb on + strand, within hofBat 116.149 kb on - strand, within hofBat 116.335 kb on + strand, within hofBat 116.399 kb on + strand, within hofBat 116.430 kb on - strand, within hofBat 116.430 kb on - strand, within hofBat 116.471 kb on - strand, within hofB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.00003125 mM
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113,564 + guaC b0104 0.11 -0.2
113,668 + guaC b0104 0.21 +1.3
113,668 + guaC b0104 0.21 +2.5
113,788 + guaC b0104 0.33 -0.5
113,788 + guaC b0104 0.33 -0.6
113,803 + guaC b0104 0.34 -2.9
113,803 + guaC b0104 0.34 +1.1
113,854 + guaC b0104 0.39 -0.6
114,020 + guaC b0104 0.55 -0.2
114,039 + guaC b0104 0.57 -2.4
114,075 + guaC b0104 0.60 +0.7
114,075 + guaC b0104 0.60 +0.0
114,082 + guaC b0104 0.61 -2.1
114,082 + guaC b0104 0.61 +2.4
114,121 - guaC b0104 0.65 +1.5
114,121 - guaC b0104 0.65 +0.7
114,191 - guaC b0104 0.72 -0.6
114,191 - guaC b0104 0.72 -0.6
114,217 - guaC b0104 0.74 -0.9
114,236 - guaC b0104 0.76 -0.6
114,246 - guaC b0104 0.77 -0.6
114,278 - guaC b0104 0.80 +1.1
114,310 + guaC b0104 0.83 -0.1
114,354 - guaC b0104 0.87 +0.9
114,399 - -2.1
114,422 - -2.8
114,480 + +1.9
114,480 + +0.4
114,509 - -0.1
114,509 - +0.7
114,519 + +2.2
114,524 + -0.2
114,527 - -0.1
114,527 - +1.4
114,532 - -0.2
114,535 - +1.2
114,545 - -1.4
114,562 + +0.9
114,627 + +0.2
114,634 + +4.0
114,680 + hofC b0106 0.13 -2.5
114,838 + hofC b0106 0.26 +0.2
114,838 + hofC b0106 0.26 +2.5
114,844 + hofC b0106 0.27 +2.3
114,928 - hofC b0106 0.34 +1.0
114,930 - +0.8
114,930 - hofC b0106 0.34 -1.1
114,998 + hofC b0106 0.40 +2.3
114,998 + hofC b0106 0.40 -0.0
115,010 - hofC b0106 0.41 -1.4
115,021 + hofC b0106 0.41 -0.4
115,021 + hofC b0106 0.41 +2.4
115,077 - hofC b0106 0.46 +0.3
115,152 - hofC b0106 0.52 -1.3
115,152 - hofC b0106 0.52 -0.6
115,201 - hofC b0106 0.56 +0.1
115,203 - hofC b0106 0.57 +0.1
115,203 - hofC b0106 0.57 +1.1
115,273 + hofC b0106 0.62 +1.1
115,278 + hofC b0106 0.63 +3.6
115,278 + hofC b0106 0.63 +1.1
115,330 + hofC b0106 0.67 -1.5
115,367 + hofC b0106 0.70 -1.6
115,367 + hofC b0106 0.70 -0.1
115,379 + hofC b0106 0.71 -0.9
115,379 + hofC b0106 0.71 +0.5
115,409 + hofC b0106 0.74 -1.8
115,474 - hofC b0106 0.79 +0.3
115,507 + hofC b0106 0.82 -0.6
115,507 + hofC b0106 0.82 +0.6
115,515 - hofC b0106 0.83 -0.9
115,624 + +1.4
115,651 + -0.9
115,699 - -0.2
115,737 + +2.4
115,856 - hofB b0107 0.10 +0.5
115,856 - hofB b0107 0.10 +0.8
115,910 + hofB b0107 0.14 -0.6
115,911 - hofB b0107 0.14 +1.1
115,911 - hofB b0107 0.14 -0.0
115,941 + hofB b0107 0.16 +0.5
115,980 + hofB b0107 0.19 +0.8
116,058 + hofB b0107 0.25 -0.2
116,149 - hofB b0107 0.31 +0.3
116,335 + hofB b0107 0.45 -0.1
116,399 + hofB b0107 0.49 +0.4
116,430 - hofB b0107 0.52 -1.5
116,430 - hofB b0107 0.52 +0.1
116,471 - hofB b0107 0.55 -1.1

Or see this region's nucleotide sequence