Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pstC and pstA overlap by 4 nucleotides pstA and pstB are separated by 43 nucleotides pstB and phoU are separated by 17 nucleotides
LU632_RS24885: pstC - phosphate ABC transporter permease PstC, at 4,747,919 to 4,748,881
pstC
LU632_RS24890: pstA - phosphate ABC transporter permease PstA, at 4,748,878 to 4,749,765
pstA
LU632_RS24895: pstB - phosphate ABC transporter ATP-binding protein PstB, at 4,749,809 to 4,750,579
pstB
LU632_RS24900: phoU - phosphate signaling complex protein PhoU, at 4,750,597 to 4,751,334
phoU
Position (kb)
4748
4749
4750 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4748.152 kb on + strand, within pstC at 4748.153 kb on - strand, within pstC at 4748.204 kb on - strand, within pstC at 4748.302 kb on + strand, within pstC at 4748.343 kb on - strand, within pstC at 4748.377 kb on + strand, within pstC at 4748.378 kb on - strand, within pstC at 4748.659 kb on + strand, within pstC at 4748.659 kb on + strand, within pstC at 4748.663 kb on - strand, within pstC at 4748.720 kb on - strand, within pstC at 4748.794 kb on + strand at 4748.929 kb on - strand at 4748.952 kb on + strand at 4749.035 kb on + strand, within pstA at 4749.276 kb on + strand, within pstA at 4749.276 kb on + strand, within pstA at 4749.277 kb on - strand, within pstA at 4749.298 kb on + strand, within pstA at 4749.440 kb on - strand, within pstA at 4749.469 kb on - strand, within pstA at 4749.469 kb on - strand, within pstA at 4749.726 kb on + strand at 4749.727 kb on - strand at 4749.763 kb on + strand at 4749.773 kb on - strand at 4749.791 kb on - strand at 4749.889 kb on + strand, within pstB at 4749.902 kb on - strand, within pstB at 4749.904 kb on + strand, within pstB at 4750.061 kb on - strand, within pstB at 4750.123 kb on + strand, within pstB at 4750.135 kb on + strand, within pstB at 4750.240 kb on + strand, within pstB at 4750.261 kb on + strand, within pstB at 4750.261 kb on + strand, within pstB at 4750.389 kb on + strand, within pstB at 4750.389 kb on + strand, within pstB at 4750.438 kb on + strand, within pstB at 4750.439 kb on - strand, within pstB at 4750.478 kb on - strand, within pstB at 4750.556 kb on + strand at 4750.556 kb on + strand at 4750.556 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 4,748,152 + pstC LU632_RS24885 0.24 -1.7 4,748,153 - pstC LU632_RS24885 0.24 -0.1 4,748,204 - pstC LU632_RS24885 0.30 -0.6 4,748,302 + pstC LU632_RS24885 0.40 -0.1 4,748,343 - pstC LU632_RS24885 0.44 -1.5 4,748,377 + pstC LU632_RS24885 0.48 -0.9 4,748,378 - pstC LU632_RS24885 0.48 -2.7 4,748,659 + pstC LU632_RS24885 0.77 -1.4 4,748,659 + pstC LU632_RS24885 0.77 -0.1 4,748,663 - pstC LU632_RS24885 0.77 -1.7 4,748,720 - pstC LU632_RS24885 0.83 -0.7 4,748,794 + -1.7 4,748,929 - -1.1 4,748,952 + -2.8 4,749,035 + pstA LU632_RS24890 0.18 -1.7 4,749,276 + pstA LU632_RS24890 0.45 -0.1 4,749,276 + pstA LU632_RS24890 0.45 -0.7 4,749,277 - pstA LU632_RS24890 0.45 -0.7 4,749,298 + pstA LU632_RS24890 0.47 -0.7 4,749,440 - pstA LU632_RS24890 0.63 -0.1 4,749,469 - pstA LU632_RS24890 0.67 -1.7 4,749,469 - pstA LU632_RS24890 0.67 -0.7 4,749,726 + -0.1 4,749,727 - -1.1 4,749,763 + +2.2 4,749,773 - -2.3 4,749,791 - -1.1 4,749,889 + pstB LU632_RS24895 0.10 -1.7 4,749,902 - pstB LU632_RS24895 0.12 -0.7 4,749,904 + pstB LU632_RS24895 0.12 -0.1 4,750,061 - pstB LU632_RS24895 0.33 -1.1 4,750,123 + pstB LU632_RS24895 0.41 -0.5 4,750,135 + pstB LU632_RS24895 0.42 -2.1 4,750,240 + pstB LU632_RS24895 0.56 -0.1 4,750,261 + pstB LU632_RS24895 0.59 -0.1 4,750,261 + pstB LU632_RS24895 0.59 -1.7 4,750,389 + pstB LU632_RS24895 0.75 +0.9 4,750,389 + pstB LU632_RS24895 0.75 -1.4 4,750,438 + pstB LU632_RS24895 0.82 -1.1 4,750,439 - pstB LU632_RS24895 0.82 -1.1 4,750,478 - pstB LU632_RS24895 0.87 -0.1 4,750,556 + -2.1 4,750,556 + -0.1 4,750,556 + -2.4
Or see this region's nucleotide sequence