Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ilvD and LU632_RS24405 are separated by 58 nucleotides LU632_RS24405 and ilvM are separated by 18 nucleotides ilvM and ilvG overlap by 4 nucleotides
LU632_RS24400: ilvD - dihydroxy-acid dehydratase, at 4,653,866 to 4,655,716
ilvD
LU632_RS24405: LU632_RS24405 - branched-chain amino acid transaminase, at 4,655,775 to 4,656,704
_RS24405
LU632_RS24410: ilvM - acetolactate synthase 2 small subunit, at 4,656,723 to 4,656,980
ilvM
LU632_RS24415: ilvG - acetolactate synthase 2 catalytic subunit, at 4,656,977 to 4,658,623
ilvG
Position (kb)
4655
4656
4657 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4654.829 kb on - strand, within ilvD at 4654.882 kb on + strand, within ilvD at 4654.883 kb on - strand, within ilvD at 4654.955 kb on + strand, within ilvD at 4655.002 kb on + strand, within ilvD at 4655.002 kb on + strand, within ilvD at 4655.003 kb on - strand, within ilvD at 4655.003 kb on - strand, within ilvD at 4655.328 kb on - strand, within ilvD at 4655.356 kb on + strand, within ilvD at 4655.400 kb on + strand, within ilvD at 4655.400 kb on + strand, within ilvD at 4655.401 kb on - strand, within ilvD at 4655.401 kb on - strand, within ilvD at 4655.443 kb on - strand, within ilvD at 4655.596 kb on + strand at 4655.675 kb on - strand at 4655.706 kb on + strand at 4655.707 kb on - strand at 4655.711 kb on - strand at 4655.711 kb on - strand at 4655.722 kb on + strand at 4655.739 kb on - strand at 4655.889 kb on + strand, within LU632_RS24405 at 4655.889 kb on + strand, within LU632_RS24405 at 4655.889 kb on + strand, within LU632_RS24405 at 4655.945 kb on + strand, within LU632_RS24405 at 4655.946 kb on - strand, within LU632_RS24405 at 4655.965 kb on + strand, within LU632_RS24405 at 4655.966 kb on - strand, within LU632_RS24405 at 4655.968 kb on - strand, within LU632_RS24405 at 4655.968 kb on - strand, within LU632_RS24405 at 4655.968 kb on - strand, within LU632_RS24405 at 4656.026 kb on + strand, within LU632_RS24405 at 4656.027 kb on - strand, within LU632_RS24405 at 4656.081 kb on - strand, within LU632_RS24405 at 4656.134 kb on + strand, within LU632_RS24405 at 4656.134 kb on + strand, within LU632_RS24405 at 4656.135 kb on - strand, within LU632_RS24405 at 4656.135 kb on - strand, within LU632_RS24405 at 4656.136 kb on + strand, within LU632_RS24405 at 4656.214 kb on + strand, within LU632_RS24405 at 4656.215 kb on - strand, within LU632_RS24405 at 4656.219 kb on - strand, within LU632_RS24405 at 4656.219 kb on - strand, within LU632_RS24405 at 4656.219 kb on - strand, within LU632_RS24405 at 4656.219 kb on - strand, within LU632_RS24405 at 4656.249 kb on - strand, within LU632_RS24405 at 4656.251 kb on + strand, within LU632_RS24405 at 4656.251 kb on + strand, within LU632_RS24405 at 4656.319 kb on + strand, within LU632_RS24405 at 4656.319 kb on + strand, within LU632_RS24405 at 4656.320 kb on - strand, within LU632_RS24405 at 4656.344 kb on + strand, within LU632_RS24405 at 4656.419 kb on + strand, within LU632_RS24405 at 4656.420 kb on - strand, within LU632_RS24405 at 4656.421 kb on + strand, within LU632_RS24405 at 4656.421 kb on + strand, within LU632_RS24405 at 4656.422 kb on - strand, within LU632_RS24405 at 4656.422 kb on - strand, within LU632_RS24405 at 4656.488 kb on - strand, within LU632_RS24405 at 4656.488 kb on - strand, within LU632_RS24405 at 4656.581 kb on - strand, within LU632_RS24405 at 4656.609 kb on - strand, within LU632_RS24405 at 4656.609 kb on - strand, within LU632_RS24405 at 4656.609 kb on - strand, within LU632_RS24405 at 4656.609 kb on - strand, within LU632_RS24405 at 4656.659 kb on + strand at 4656.660 kb on - strand at 4656.660 kb on - strand at 4656.683 kb on + strand at 4656.684 kb on - strand at 4656.741 kb on + strand at 4656.742 kb on - strand at 4656.742 kb on - strand at 4656.766 kb on + strand, within ilvM at 4656.767 kb on - strand, within ilvM at 4656.788 kb on + strand, within ilvM at 4656.804 kb on + strand, within ilvM at 4656.884 kb on + strand, within ilvM at 4656.884 kb on + strand, within ilvM at 4656.885 kb on - strand, within ilvM at 4656.885 kb on - strand, within ilvM at 4657.071 kb on + strand at 4657.071 kb on + strand at 4657.071 kb on + strand at 4657.071 kb on + strand at 4657.072 kb on - strand at 4657.072 kb on - strand at 4657.072 kb on - strand at 4657.072 kb on - strand at 4657.072 kb on - strand at 4657.072 kb on - strand at 4657.072 kb on - strand at 4657.085 kb on - strand at 4657.100 kb on + strand at 4657.101 kb on - strand at 4657.101 kb on - strand at 4657.101 kb on - strand at 4657.101 kb on - strand at 4657.123 kb on + strand at 4657.124 kb on - strand at 4657.124 kb on - strand at 4657.129 kb on + strand at 4657.129 kb on + strand at 4657.129 kb on + strand at 4657.130 kb on - strand at 4657.132 kb on + strand at 4657.204 kb on + strand, within ilvG at 4657.207 kb on - strand, within ilvG at 4657.364 kb on - strand, within ilvG at 4657.438 kb on + strand, within ilvG at 4657.468 kb on + strand, within ilvG at 4657.468 kb on + strand, within ilvG at 4657.468 kb on + strand, within ilvG at 4657.468 kb on + strand, within ilvG at 4657.468 kb on + strand, within ilvG at 4657.469 kb on - strand, within ilvG at 4657.469 kb on - strand, within ilvG at 4657.469 kb on - strand, within ilvG at 4657.469 kb on - strand, within ilvG at 4657.469 kb on - strand, within ilvG at 4657.469 kb on - strand, within ilvG at 4657.469 kb on - strand, within ilvG at 4657.566 kb on + strand, within ilvG at 4657.566 kb on + strand, within ilvG at 4657.566 kb on + strand, within ilvG at 4657.566 kb on + strand, within ilvG at 4657.567 kb on - strand, within ilvG at 4657.567 kb on - strand, within ilvG at 4657.567 kb on - strand, within ilvG at 4657.591 kb on - strand, within ilvG at 4657.659 kb on + strand, within ilvG at 4657.660 kb on - strand, within ilvG at 4657.660 kb on - strand, within ilvG
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 4,654,829 - ilvD LU632_RS24400 0.52 +0.4 4,654,882 + ilvD LU632_RS24400 0.55 -0.7 4,654,883 - ilvD LU632_RS24400 0.55 -0.1 4,654,955 + ilvD LU632_RS24400 0.59 +0.2 4,655,002 + ilvD LU632_RS24400 0.61 -0.1 4,655,002 + ilvD LU632_RS24400 0.61 +0.1 4,655,003 - ilvD LU632_RS24400 0.61 +0.0 4,655,003 - ilvD LU632_RS24400 0.61 +0.3 4,655,328 - ilvD LU632_RS24400 0.79 -0.1 4,655,356 + ilvD LU632_RS24400 0.80 -0.1 4,655,400 + ilvD LU632_RS24400 0.83 -1.7 4,655,400 + ilvD LU632_RS24400 0.83 -1.1 4,655,401 - ilvD LU632_RS24400 0.83 +0.1 4,655,401 - ilvD LU632_RS24400 0.83 +0.3 4,655,443 - ilvD LU632_RS24400 0.85 -0.6 4,655,596 + -2.1 4,655,675 - +0.5 4,655,706 + -0.0 4,655,707 - +0.6 4,655,711 - -0.1 4,655,711 - +0.5 4,655,722 + +0.9 4,655,739 - +1.5 4,655,889 + LU632_RS24405 0.12 +0.2 4,655,889 + LU632_RS24405 0.12 -0.2 4,655,889 + LU632_RS24405 0.12 -0.1 4,655,945 + LU632_RS24405 0.18 +1.5 4,655,946 - LU632_RS24405 0.18 +1.5 4,655,965 + LU632_RS24405 0.20 -1.0 4,655,966 - LU632_RS24405 0.21 +0.1 4,655,968 - LU632_RS24405 0.21 -2.1 4,655,968 - LU632_RS24405 0.21 -1.1 4,655,968 - LU632_RS24405 0.21 -0.7 4,656,026 + LU632_RS24405 0.27 -2.4 4,656,027 - LU632_RS24405 0.27 +0.6 4,656,081 - LU632_RS24405 0.33 -0.1 4,656,134 + LU632_RS24405 0.39 -0.3 4,656,134 + LU632_RS24405 0.39 -0.7 4,656,135 - LU632_RS24405 0.39 +1.5 4,656,135 - LU632_RS24405 0.39 +0.5 4,656,136 + LU632_RS24405 0.39 +0.9 4,656,214 + LU632_RS24405 0.47 +0.3 4,656,215 - LU632_RS24405 0.47 +0.6 4,656,219 - LU632_RS24405 0.48 +0.5 4,656,219 - LU632_RS24405 0.48 -0.5 4,656,219 - LU632_RS24405 0.48 -1.1 4,656,219 - LU632_RS24405 0.48 +0.2 4,656,249 - LU632_RS24405 0.51 +0.7 4,656,251 + LU632_RS24405 0.51 -0.9 4,656,251 + LU632_RS24405 0.51 +1.1 4,656,319 + LU632_RS24405 0.58 +0.9 4,656,319 + LU632_RS24405 0.58 -0.5 4,656,320 - LU632_RS24405 0.59 +0.4 4,656,344 + LU632_RS24405 0.61 -0.9 4,656,419 + LU632_RS24405 0.69 -0.2 4,656,420 - LU632_RS24405 0.69 -0.3 4,656,421 + LU632_RS24405 0.69 -0.3 4,656,421 + LU632_RS24405 0.69 -0.1 4,656,422 - LU632_RS24405 0.70 +0.4 4,656,422 - LU632_RS24405 0.70 -0.6 4,656,488 - LU632_RS24405 0.77 -0.5 4,656,488 - LU632_RS24405 0.77 -0.1 4,656,581 - LU632_RS24405 0.87 -0.1 4,656,609 - LU632_RS24405 0.90 -2.1 4,656,609 - LU632_RS24405 0.90 -0.5 4,656,609 - LU632_RS24405 0.90 -0.9 4,656,609 - LU632_RS24405 0.90 +1.5 4,656,659 + -0.6 4,656,660 - +0.3 4,656,660 - -0.0 4,656,683 + -0.1 4,656,684 - -0.6 4,656,741 + +0.6 4,656,742 - +0.2 4,656,742 - -0.4 4,656,766 + ilvM LU632_RS24410 0.17 +0.9 4,656,767 - ilvM LU632_RS24410 0.17 -0.9 4,656,788 + ilvM LU632_RS24410 0.25 -0.6 4,656,804 + ilvM LU632_RS24410 0.31 +0.9 4,656,884 + ilvM LU632_RS24410 0.62 +0.9 4,656,884 + ilvM LU632_RS24410 0.62 +1.1 4,656,885 - ilvM LU632_RS24410 0.63 +0.5 4,656,885 - ilvM LU632_RS24410 0.63 +1.0 4,657,071 + +1.2 4,657,071 + +0.9 4,657,071 + -0.1 4,657,071 + +0.1 4,657,072 - +0.2 4,657,072 - -0.7 4,657,072 - +0.6 4,657,072 - -0.1 4,657,072 - -0.1 4,657,072 - -0.2 4,657,072 - +0.5 4,657,085 - -0.5 4,657,100 + +0.6 4,657,101 - +0.6 4,657,101 - -0.1 4,657,101 - +0.1 4,657,101 - +0.6 4,657,123 + +0.3 4,657,124 - +1.5 4,657,124 - +1.5 4,657,129 + -0.1 4,657,129 + -0.3 4,657,129 + -1.2 4,657,130 - +0.5 4,657,132 + -0.7 4,657,204 + ilvG LU632_RS24415 0.14 -2.7 4,657,207 - ilvG LU632_RS24415 0.14 -0.3 4,657,364 - ilvG LU632_RS24415 0.23 -0.5 4,657,438 + ilvG LU632_RS24415 0.28 +0.4 4,657,468 + ilvG LU632_RS24415 0.30 +0.3 4,657,468 + ilvG LU632_RS24415 0.30 -1.1 4,657,468 + ilvG LU632_RS24415 0.30 -0.1 4,657,468 + ilvG LU632_RS24415 0.30 -1.3 4,657,468 + ilvG LU632_RS24415 0.30 +0.1 4,657,469 - ilvG LU632_RS24415 0.30 +0.7 4,657,469 - ilvG LU632_RS24415 0.30 +0.5 4,657,469 - ilvG LU632_RS24415 0.30 -0.7 4,657,469 - ilvG LU632_RS24415 0.30 -2.7 4,657,469 - ilvG LU632_RS24415 0.30 -0.7 4,657,469 - ilvG LU632_RS24415 0.30 +1.4 4,657,469 - ilvG LU632_RS24415 0.30 -1.1 4,657,566 + ilvG LU632_RS24415 0.36 +1.9 4,657,566 + ilvG LU632_RS24415 0.36 +1.1 4,657,566 + ilvG LU632_RS24415 0.36 +0.2 4,657,566 + ilvG LU632_RS24415 0.36 -0.1 4,657,567 - ilvG LU632_RS24415 0.36 -1.9 4,657,567 - ilvG LU632_RS24415 0.36 -1.5 4,657,567 - ilvG LU632_RS24415 0.36 -0.3 4,657,591 - ilvG LU632_RS24415 0.37 -0.9 4,657,659 + ilvG LU632_RS24415 0.41 -1.3 4,657,660 - ilvG LU632_RS24415 0.41 -1.3 4,657,660 - ilvG LU632_RS24415 0.41 +2.5
Or see this region's nucleotide sequence