Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tatB and tatA are separated by 3 nucleotides tatA and ubiB are separated by 115 nucleotides ubiB and LU632_RS24000 overlap by 4 nucleotides LU632_RS24000 and ubiE are separated by 12 nucleotides
LU632_RS23985: tatB - Sec-independent protein translocase protein TatB, at 4,572,834 to 4,573,481
tatB
LU632_RS23990: tatA - twin-arginine translocase TatA/TatE family subunit, at 4,573,485 to 4,573,733
tatA
LU632_RS23995: ubiB - ubiquinone biosynthesis regulatory protein kinase UbiB, at 4,573,849 to 4,575,486
ubiB
LU632_RS24000: LU632_RS24000 - SCP2 domain-containing protein, at 4,575,483 to 4,576,088
_RS24000
LU632_RS24005: ubiE - bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE, at 4,576,101 to 4,576,856
ubiE
Position (kb)
4573
4574
4575
4576 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4572.959 kb on + strand, within tatB at 4573.148 kb on + strand, within tatB at 4573.194 kb on - strand, within tatB at 4573.195 kb on + strand, within tatB at 4573.196 kb on - strand, within tatB at 4573.196 kb on - strand, within tatB at 4573.196 kb on - strand, within tatB at 4573.248 kb on - strand, within tatB at 4573.320 kb on + strand, within tatB at 4573.423 kb on - strand at 4573.452 kb on + strand at 4573.471 kb on - strand at 4573.487 kb on - strand at 4573.661 kb on + strand, within tatA at 4573.662 kb on - strand, within tatA at 4573.722 kb on - strand at 4573.776 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 4,572,959 + tatB LU632_RS23985 0.19 -1.7 4,573,148 + tatB LU632_RS23985 0.48 -2.5 4,573,194 - tatB LU632_RS23985 0.56 +1.5 4,573,195 + tatB LU632_RS23985 0.56 -3.1 4,573,196 - tatB LU632_RS23985 0.56 +0.2 4,573,196 - tatB LU632_RS23985 0.56 -0.1 4,573,196 - tatB LU632_RS23985 0.56 +0.8 4,573,248 - tatB LU632_RS23985 0.64 -0.1 4,573,320 + tatB LU632_RS23985 0.75 -3.6 4,573,423 - -3.7 4,573,452 + -4.0 4,573,471 - -3.4 4,573,487 - -3.5 4,573,661 + tatA LU632_RS23990 0.71 -0.7 4,573,662 - tatA LU632_RS23990 0.71 -0.3 4,573,722 - -1.3 4,573,776 - +0.2
Or see this region's nucleotide sequence