Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fadA and fre are separated by 86 nucleotides fre and ubiD are separated by 25 nucleotides ubiD and rfaH are separated by 277 nucleotides rfaH and tatD are separated by 21 nucleotides
LU632_RS23955: fadA - acetyl-CoA C-acyltransferase, at 4,567,773 to 4,568,100
fadA
LU632_RS23960: fre - NAD(P)H-flavin reductase, at 4,568,187 to 4,568,888
fre
LU632_RS23965: ubiD - 4-hydroxy-3-polyprenylbenzoate decarboxylase, at 4,568,914 to 4,570,416
ubiD
LU632_RS23970: rfaH - transcription/translation regulatory transformer protein RfaH, at 4,570,694 to 4,571,182
rfaH
LU632_RS23975: tatD - 3'-5' ssDNA/RNA exonuclease TatD, at 4,571,204 to 4,571,998
tatD
Position (kb)
4568
4569
4570
4571 Strain fitness (log2 ratio)
-2
-1
0
1 at 4568.055 kb on + strand, within fadA at 4568.056 kb on - strand, within fadA at 4568.125 kb on - strand at 4568.167 kb on + strand at 4570.428 kb on - strand at 4570.436 kb on - strand at 4570.453 kb on - strand at 4570.535 kb on - strand at 4570.537 kb on + strand at 4570.537 kb on + strand at 4570.538 kb on - strand at 4570.542 kb on - strand at 4570.579 kb on - strand at 4570.579 kb on - strand at 4570.579 kb on - strand at 4570.660 kb on - strand at 4570.665 kb on + strand at 4570.669 kb on - strand at 4570.710 kb on + strand at 4570.711 kb on - strand at 4570.825 kb on + strand, within rfaH at 4570.828 kb on - strand, within rfaH at 4570.861 kb on + strand, within rfaH at 4570.882 kb on + strand, within rfaH at 4570.883 kb on + strand, within rfaH at 4570.883 kb on - strand, within rfaH at 4571.004 kb on + strand, within rfaH at 4571.040 kb on + strand, within rfaH at 4571.040 kb on + strand, within rfaH at 4571.041 kb on - strand, within rfaH at 4571.045 kb on - strand, within rfaH at 4571.045 kb on - strand, within rfaH at 4571.205 kb on + strand at 4571.311 kb on + strand, within tatD at 4571.311 kb on + strand, within tatD at 4571.380 kb on - strand, within tatD
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 4,568,055 + fadA LU632_RS23955 0.86 +0.9 4,568,056 - fadA LU632_RS23955 0.86 -1.1 4,568,125 - +0.4 4,568,167 + +0.3 4,570,428 - +0.7 4,570,436 - +1.0 4,570,453 - -1.9 4,570,535 - +0.1 4,570,537 + +0.2 4,570,537 + +1.1 4,570,538 - -0.1 4,570,542 - -0.8 4,570,579 - -0.5 4,570,579 - -0.4 4,570,579 - -0.4 4,570,660 - +0.5 4,570,665 + +0.3 4,570,669 - +1.0 4,570,710 + +0.9 4,570,711 - +0.4 4,570,825 + rfaH LU632_RS23970 0.27 -0.7 4,570,828 - rfaH LU632_RS23970 0.27 +0.3 4,570,861 + rfaH LU632_RS23970 0.34 -1.1 4,570,882 + rfaH LU632_RS23970 0.38 -0.1 4,570,883 + rfaH LU632_RS23970 0.39 +0.3 4,570,883 - rfaH LU632_RS23970 0.39 +0.4 4,571,004 + rfaH LU632_RS23970 0.63 +0.2 4,571,040 + rfaH LU632_RS23970 0.71 +0.0 4,571,040 + rfaH LU632_RS23970 0.71 -1.7 4,571,041 - rfaH LU632_RS23970 0.71 +0.2 4,571,045 - rfaH LU632_RS23970 0.72 +0.2 4,571,045 - rfaH LU632_RS23970 0.72 +0.2 4,571,205 + -0.1 4,571,311 + tatD LU632_RS23975 0.13 +0.3 4,571,311 + tatD LU632_RS23975 0.13 -1.1 4,571,380 - tatD LU632_RS23975 0.22 +1.2
Or see this region's nucleotide sequence