Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgi and LU632_RS22370 are separated by 164 nucleotides LU632_RS22370 and lysC are separated by 563 nucleotides lysC and LU632_RS22380 are separated by 648 nucleotides
LU632_RS22365: pgi - glucose-6-phosphate isomerase, at 4,271,103 to 4,272,752
pgi
LU632_RS22370: LU632_RS22370 - hypothetical protein, at 4,272,917 to 4,273,075
_RS22370
LU632_RS22375: lysC - lysine-sensitive aspartokinase 3, at 4,273,639 to 4,274,994
lysC
LU632_RS22380: LU632_RS22380 - bile acid:sodium symporter family protein, at 4,275,643 to 4,276,560
_RS22380
Position (kb)
4273
4274
4275 Strain fitness (log2 ratio)
-2
-1
0
1 at 4272.783 kb on + strand at 4272.800 kb on + strand at 4272.800 kb on + strand at 4272.800 kb on + strand at 4272.801 kb on - strand at 4272.801 kb on - strand at 4272.801 kb on - strand at 4272.911 kb on - strand at 4273.051 kb on - strand, within LU632_RS22370 at 4273.184 kb on - strand at 4273.189 kb on - strand at 4273.253 kb on + strand at 4273.253 kb on + strand at 4273.268 kb on - strand at 4273.380 kb on + strand at 4273.466 kb on + strand at 4273.552 kb on - strand at 4273.613 kb on + strand at 4273.629 kb on - strand at 4273.878 kb on - strand, within lysC at 4273.927 kb on - strand, within lysC at 4273.966 kb on - strand, within lysC at 4273.966 kb on - strand, within lysC at 4274.178 kb on + strand, within lysC at 4274.179 kb on - strand, within lysC at 4274.227 kb on + strand, within lysC at 4274.227 kb on - strand, within lysC at 4274.227 kb on - strand, within lysC at 4274.251 kb on + strand, within lysC at 4274.323 kb on + strand, within lysC at 4274.323 kb on + strand, within lysC at 4274.591 kb on + strand, within lysC at 4274.592 kb on - strand, within lysC at 4274.592 kb on - strand, within lysC at 4274.595 kb on + strand, within lysC at 4274.596 kb on - strand, within lysC at 4274.596 kb on - strand, within lysC at 4274.649 kb on + strand, within lysC at 4274.649 kb on + strand, within lysC at 4274.655 kb on - strand, within lysC at 4274.671 kb on - strand, within lysC at 4274.775 kb on - strand, within lysC at 4274.817 kb on + strand, within lysC at 4274.902 kb on + strand at 4274.917 kb on + strand at 4274.917 kb on + strand at 4274.918 kb on - strand at 4274.918 kb on - strand at 4274.980 kb on - strand at 4275.007 kb on + strand at 4275.075 kb on + strand at 4275.075 kb on + strand at 4275.098 kb on + strand at 4275.165 kb on + strand at 4275.166 kb on - strand at 4275.232 kb on - strand at 4275.232 kb on - strand at 4275.232 kb on - strand at 4275.274 kb on + strand at 4275.275 kb on - strand at 4275.280 kb on - strand at 4275.348 kb on + strand at 4275.348 kb on + strand at 4275.349 kb on - strand at 4275.354 kb on + strand at 4275.447 kb on + strand at 4275.521 kb on + strand at 4275.522 kb on - strand at 4275.522 kb on - strand at 4275.522 kb on - strand at 4275.544 kb on + strand at 4275.658 kb on - strand at 4275.704 kb on - strand at 4275.748 kb on + strand, within LU632_RS22380 at 4275.749 kb on - strand, within LU632_RS22380 at 4275.766 kb on - strand, within LU632_RS22380 at 4275.766 kb on - strand, within LU632_RS22380 at 4275.766 kb on - strand, within LU632_RS22380 at 4275.860 kb on + strand, within LU632_RS22380 at 4275.891 kb on + strand, within LU632_RS22380 at 4275.904 kb on - strand, within LU632_RS22380 at 4275.904 kb on - strand, within LU632_RS22380 at 4275.969 kb on + strand, within LU632_RS22380 at 4275.970 kb on - strand, within LU632_RS22380
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 4,272,783 + +0.2 4,272,800 + +0.9 4,272,800 + +1.4 4,272,800 + +0.3 4,272,801 - -0.2 4,272,801 - +0.6 4,272,801 - -0.4 4,272,911 - +0.1 4,273,051 - LU632_RS22370 0.84 +0.8 4,273,184 - -1.2 4,273,189 - -0.4 4,273,253 + -0.2 4,273,253 + -0.4 4,273,268 - -0.2 4,273,380 + -0.1 4,273,466 + -0.2 4,273,552 - -0.2 4,273,613 + +0.5 4,273,629 - -0.2 4,273,878 - lysC LU632_RS22375 0.18 +0.8 4,273,927 - lysC LU632_RS22375 0.21 -0.2 4,273,966 - lysC LU632_RS22375 0.24 -0.2 4,273,966 - lysC LU632_RS22375 0.24 -1.2 4,274,178 + lysC LU632_RS22375 0.40 -1.2 4,274,179 - lysC LU632_RS22375 0.40 -1.2 4,274,227 + lysC LU632_RS22375 0.43 -1.2 4,274,227 - lysC LU632_RS22375 0.43 +0.8 4,274,227 - lysC LU632_RS22375 0.43 -1.2 4,274,251 + lysC LU632_RS22375 0.45 -1.2 4,274,323 + lysC LU632_RS22375 0.50 -1.2 4,274,323 + lysC LU632_RS22375 0.50 -1.2 4,274,591 + lysC LU632_RS22375 0.70 -0.7 4,274,592 - lysC LU632_RS22375 0.70 -1.6 4,274,592 - lysC LU632_RS22375 0.70 -0.7 4,274,595 + lysC LU632_RS22375 0.71 +0.8 4,274,596 - lysC LU632_RS22375 0.71 +1.4 4,274,596 - lysC LU632_RS22375 0.71 -0.2 4,274,649 + lysC LU632_RS22375 0.74 -1.2 4,274,649 + lysC LU632_RS22375 0.74 -0.9 4,274,655 - lysC LU632_RS22375 0.75 -2.5 4,274,671 - lysC LU632_RS22375 0.76 -0.2 4,274,775 - lysC LU632_RS22375 0.84 -1.4 4,274,817 + lysC LU632_RS22375 0.87 -1.2 4,274,902 + -0.2 4,274,917 + -1.7 4,274,917 + -2.2 4,274,918 - -1.2 4,274,918 - -0.2 4,274,980 - -2.5 4,275,007 + -0.6 4,275,075 + -1.1 4,275,075 + +1.2 4,275,098 + -1.2 4,275,165 + -0.3 4,275,166 - +0.1 4,275,232 - +0.9 4,275,232 - -0.9 4,275,232 - -0.6 4,275,274 + -1.0 4,275,275 - +0.1 4,275,280 - -2.2 4,275,348 + +1.4 4,275,348 + -0.6 4,275,349 - -0.5 4,275,354 + +0.6 4,275,447 + +0.1 4,275,521 + +1.0 4,275,522 - -0.7 4,275,522 - +0.1 4,275,522 - +0.1 4,275,544 + +0.1 4,275,658 - +0.8 4,275,704 - +0.3 4,275,748 + LU632_RS22380 0.11 -0.7 4,275,749 - LU632_RS22380 0.12 +0.4 4,275,766 - LU632_RS22380 0.13 -0.0 4,275,766 - LU632_RS22380 0.13 -0.3 4,275,766 - LU632_RS22380 0.13 -0.5 4,275,860 + LU632_RS22380 0.24 +0.3 4,275,891 + LU632_RS22380 0.27 +0.8 4,275,904 - LU632_RS22380 0.28 -0.2 4,275,904 - LU632_RS22380 0.28 +0.4 4,275,969 + LU632_RS22380 0.36 -0.0 4,275,970 - LU632_RS22380 0.36 -0.0
Or see this region's nucleotide sequence