Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt msrP and msrQ overlap by 1 nucleotides msrQ and aroQ are separated by 237 nucleotides aroQ and accB are separated by 21 nucleotides accB and accC are separated by 11 nucleotides
LU632_RS21465: msrP - protein-methionine-sulfoxide reductase catalytic subunit MsrP, at 4,107,034 to 4,108,035
msrP
LU632_RS21470: msrQ - protein-methionine-sulfoxide reductase heme-binding subunit MsrQ, at 4,108,035 to 4,108,637
msrQ
LU632_RS21475: aroQ - type II 3-dehydroquinate dehydratase, at 4,108,875 to 4,109,327
aroQ
LU632_RS21480: accB - acetyl-CoA carboxylase biotin carboxyl carrier protein, at 4,109,349 to 4,109,822
accB
LU632_RS21485: accC - acetyl-CoA carboxylase biotin carboxylase subunit, at 4,109,834 to 4,111,183
accC
Position (kb)
4108
4109
4110 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4107.947 kb on + strand at 4107.965 kb on + strand at 4107.966 kb on - strand at 4108.049 kb on + strand at 4108.052 kb on + strand at 4108.052 kb on + strand at 4108.058 kb on + strand at 4108.058 kb on + strand at 4108.058 kb on + strand at 4108.059 kb on - strand at 4108.059 kb on - strand at 4108.059 kb on - strand at 4108.059 kb on - strand at 4108.059 kb on - strand at 4108.321 kb on - strand, within msrQ at 4108.331 kb on - strand, within msrQ at 4108.331 kb on - strand, within msrQ at 4108.386 kb on + strand, within msrQ at 4108.415 kb on + strand, within msrQ at 4108.416 kb on - strand, within msrQ at 4108.484 kb on - strand, within msrQ at 4108.493 kb on + strand, within msrQ at 4108.501 kb on - strand, within msrQ at 4108.531 kb on - strand, within msrQ at 4108.573 kb on - strand, within msrQ at 4108.722 kb on - strand at 4108.734 kb on + strand at 4108.761 kb on + strand at 4108.761 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 4,107,947 + -0.5 4,107,965 + -0.8 4,107,966 - +0.8 4,108,049 + +0.2 4,108,052 + +0.8 4,108,052 + -1.2 4,108,058 + +0.2 4,108,058 + -0.7 4,108,058 + +1.3 4,108,059 - -0.4 4,108,059 - -0.5 4,108,059 - +0.5 4,108,059 - +0.5 4,108,059 - +0.7 4,108,321 - msrQ LU632_RS21470 0.47 +1.8 4,108,331 - msrQ LU632_RS21470 0.49 -0.8 4,108,331 - msrQ LU632_RS21470 0.49 +0.1 4,108,386 + msrQ LU632_RS21470 0.58 +0.3 4,108,415 + msrQ LU632_RS21470 0.63 -0.2 4,108,416 - msrQ LU632_RS21470 0.63 +0.4 4,108,484 - msrQ LU632_RS21470 0.74 +0.4 4,108,493 + msrQ LU632_RS21470 0.76 +1.2 4,108,501 - msrQ LU632_RS21470 0.77 -0.6 4,108,531 - msrQ LU632_RS21470 0.82 -0.1 4,108,573 - msrQ LU632_RS21470 0.89 -0.4 4,108,722 - -2.0 4,108,734 + -0.5 4,108,761 + +0.4 4,108,761 + -0.6
Or see this region's nucleotide sequence