Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS21155 and LU632_RS21160 are separated by 37 nucleotides LU632_RS21160 and greA are separated by 173 nucleotides greA and dacB are separated by 249 nucleotides
LU632_RS21155: LU632_RS21155 - IS1 family transposase, at 4,042,191 to 4,042,888
_RS21155
LU632_RS21160: LU632_RS21160 - tyrosine-type recombinase/integrase, at 4,042,926 to 4,043,423
_RS21160
LU632_RS21165: greA - transcription elongation factor GreA, at 4,043,597 to 4,044,073
greA
LU632_RS21170: dacB - serine-type D-Ala-D-Ala carboxypeptidase, at 4,044,323 to 4,045,756
dacB
Position (kb) 4043
4044
4045 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4043.660 kb on - strand, within greA at 4043.662 kb on + strand, within greA at 4043.663 kb on - strand, within greA at 4043.760 kb on + strand, within greA at 4043.789 kb on + strand, within greA at 4043.818 kb on + strand, within greA at 4043.818 kb on + strand, within greA at 4043.853 kb on - strand, within greA at 4043.986 kb on + strand, within greA at 4044.087 kb on - strand at 4044.165 kb on + strand at 4044.166 kb on - strand at 4044.166 kb on - strand at 4044.166 kb on - strand at 4044.193 kb on + strand at 4044.193 kb on + strand at 4044.193 kb on + strand at 4044.195 kb on + strand at 4044.195 kb on + strand at 4044.196 kb on - strand at 4044.196 kb on - strand at 4044.196 kb on - strand at 4044.230 kb on + strand at 4044.230 kb on + strand at 4044.230 kb on + strand at 4044.231 kb on - strand at 4044.264 kb on + strand at 4044.265 kb on - strand at 4044.265 kb on - strand at 4044.310 kb on + strand at 4044.319 kb on + strand at 4044.340 kb on + strand at 4044.398 kb on + strand at 4044.399 kb on - strand at 4044.399 kb on - strand at 4044.438 kb on + strand at 4044.438 kb on + strand at 4044.438 kb on + strand at 4044.439 kb on - strand at 4044.439 kb on - strand at 4044.439 kb on - strand at 4044.471 kb on + strand, within dacB at 4044.649 kb on + strand, within dacB at 4044.650 kb on - strand, within dacB at 4044.654 kb on + strand, within dacB at 4044.655 kb on - strand, within dacB at 4044.655 kb on - strand, within dacB at 4044.657 kb on + strand, within dacB at 4044.881 kb on - strand, within dacB at 4044.909 kb on - strand, within dacB at 4044.926 kb on - strand, within dacB at 4044.937 kb on + strand, within dacB at 4044.938 kb on - strand, within dacB
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 4,043,660 - greA LU632_RS21165 0.13 -2.5 4,043,662 + greA LU632_RS21165 0.14 -1.9 4,043,663 - greA LU632_RS21165 0.14 -1.2 4,043,760 + greA LU632_RS21165 0.34 -2.0 4,043,789 + greA LU632_RS21165 0.40 -1.4 4,043,818 + greA LU632_RS21165 0.46 -2.2 4,043,818 + greA LU632_RS21165 0.46 -1.2 4,043,853 - greA LU632_RS21165 0.54 -2.5 4,043,986 + greA LU632_RS21165 0.82 -1.2 4,044,087 - +0.6 4,044,165 + +1.4 4,044,166 - -0.2 4,044,166 - -1.8 4,044,166 - -0.7 4,044,193 + -0.8 4,044,193 + +0.3 4,044,193 + -0.2 4,044,195 + -0.9 4,044,195 + -0.2 4,044,196 - +0.1 4,044,196 - -1.6 4,044,196 - +0.1 4,044,230 + -0.5 4,044,230 + -0.3 4,044,230 + -0.1 4,044,231 - -0.2 4,044,264 + +0.8 4,044,265 - +0.6 4,044,265 - -0.2 4,044,310 + +0.6 4,044,319 + +0.1 4,044,340 + +0.3 4,044,398 + -0.0 4,044,399 - +0.5 4,044,399 - +0.3 4,044,438 + +0.7 4,044,438 + +0.5 4,044,438 + +2.1 4,044,439 - +0.7 4,044,439 - +0.5 4,044,439 - +0.5 4,044,471 + dacB LU632_RS21170 0.10 +0.1 4,044,649 + dacB LU632_RS21170 0.23 -1.2 4,044,650 - dacB LU632_RS21170 0.23 +0.3 4,044,654 + dacB LU632_RS21170 0.23 -0.2 4,044,655 - dacB LU632_RS21170 0.23 +0.4 4,044,655 - dacB LU632_RS21170 0.23 -0.2 4,044,657 + dacB LU632_RS21170 0.23 +0.4 4,044,881 - dacB LU632_RS21170 0.39 +0.0 4,044,909 - dacB LU632_RS21170 0.41 +1.4 4,044,926 - dacB LU632_RS21170 0.42 -0.4 4,044,937 + dacB LU632_RS21170 0.43 +0.4 4,044,938 - dacB LU632_RS21170 0.43 -0.9
Or see this region's nucleotide sequence