Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nusA and rimP are separated by 24 nucleotides rimP and LU632_RS21075 are separated by 208 nucleotides LU632_RS21075 and LU632_RS21080 are separated by 59 nucleotides LU632_RS21080 and secG are separated by 50 nucleotides secG and glmM are separated by 301 nucleotides
LU632_RS21065: nusA - transcription termination factor NusA, at 4,027,888 to 4,029,393
nusA
LU632_RS21070: rimP - ribosome maturation factor RimP, at 4,029,418 to 4,029,870
rimP
LU632_RS21075: LU632_RS21075 - tRNA-Met, at 4,030,079 to 4,030,155
_RS21075
LU632_RS21080: LU632_RS21080 - tRNA-Leu, at 4,030,215 to 4,030,301
_RS21080
LU632_RS21085: secG - preprotein translocase subunit SecG, at 4,030,352 to 4,030,687
secG
LU632_RS21090: glmM - phosphoglucosamine mutase, at 4,030,989 to 4,032,323
glmM
Position (kb)
4030
4031 Strain fitness (log2 ratio)
-2
-1
0
1 at 4029.756 kb on - strand, within rimP at 4029.785 kb on - strand, within rimP at 4029.901 kb on - strand at 4029.980 kb on - strand at 4030.033 kb on - strand at 4030.041 kb on - strand at 4030.055 kb on - strand at 4030.472 kb on - strand, within secG at 4030.490 kb on - strand, within secG at 4030.653 kb on - strand, within secG at 4030.688 kb on - strand at 4030.734 kb on - strand at 4030.816 kb on - strand at 4030.826 kb on + strand at 4030.826 kb on + strand at 4030.911 kb on - strand at 4030.946 kb on + strand at 4030.960 kb on + strand at 4030.983 kb on + strand at 4030.986 kb on + strand at 4030.987 kb on - strand at 4030.990 kb on + strand at 4030.990 kb on + strand at 4030.990 kb on + strand at 4030.991 kb on - strand at 4030.991 kb on - strand at 4031.000 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 4,029,756 - rimP LU632_RS21070 0.75 -0.2 4,029,785 - rimP LU632_RS21070 0.81 -0.2 4,029,901 - +0.7 4,029,980 - +1.7 4,030,033 - +1.0 4,030,041 - -0.5 4,030,055 - -1.0 4,030,472 - secG LU632_RS21085 0.36 -1.0 4,030,490 - secG LU632_RS21085 0.41 -1.8 4,030,653 - secG LU632_RS21085 0.90 -2.2 4,030,688 - -0.7 4,030,734 - +1.0 4,030,816 - +1.3 4,030,826 + -0.2 4,030,826 + +0.3 4,030,911 - -0.3 4,030,946 + -1.8 4,030,960 + +0.0 4,030,983 + +0.2 4,030,986 + +1.0 4,030,987 - +0.2 4,030,990 + -0.6 4,030,990 + -2.3 4,030,990 + +0.5 4,030,991 - -0.2 4,030,991 - -1.5 4,031,000 + +1.2
Or see this region's nucleotide sequence