Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt exbD and exbB are separated by 5 nucleotides exbB and metC are separated by 233 nucleotides metC and LU632_RS20550 are separated by 96 nucleotides
LU632_RS20535: exbD - TonB system transport protein ExbD, at 3,909,890 to 3,910,312
exbD
LU632_RS20540: exbB - tol-pal system-associated acyl-CoA thioesterase, at 3,910,318 to 3,911,052
exbB
LU632_RS20545: metC - cystathionine beta-lyase, at 3,911,286 to 3,912,485
metC
LU632_RS20550: LU632_RS20550 - DedA family protein, at 3,912,582 to 3,913,238
_RS20550
Position (kb)
3911
3912
3913 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3910.298 kb on + strand at 3910.299 kb on - strand at 3910.362 kb on + strand at 3910.363 kb on - strand at 3910.469 kb on + strand, within exbB at 3910.470 kb on - strand, within exbB at 3910.486 kb on + strand, within exbB at 3910.579 kb on - strand, within exbB at 3910.606 kb on + strand, within exbB at 3911.006 kb on + strand at 3911.006 kb on + strand at 3911.006 kb on + strand at 3911.007 kb on - strand at 3911.007 kb on - strand at 3911.207 kb on + strand at 3911.207 kb on + strand at 3911.447 kb on - strand, within metC at 3911.451 kb on + strand, within metC at 3911.451 kb on + strand, within metC at 3911.615 kb on - strand, within metC at 3911.650 kb on + strand, within metC at 3911.881 kb on - strand, within metC at 3911.891 kb on + strand, within metC at 3911.891 kb on + strand, within metC at 3911.891 kb on + strand, within metC at 3911.912 kb on + strand, within metC at 3911.912 kb on + strand, within metC at 3911.984 kb on - strand, within metC at 3911.997 kb on + strand, within metC at 3911.997 kb on + strand, within metC at 3911.998 kb on - strand, within metC at 3912.003 kb on - strand, within metC at 3912.033 kb on + strand, within metC at 3912.033 kb on + strand, within metC at 3912.033 kb on + strand, within metC at 3912.034 kb on - strand, within metC at 3912.034 kb on - strand, within metC at 3912.034 kb on - strand, within metC at 3912.068 kb on - strand, within metC at 3912.158 kb on - strand, within metC at 3912.288 kb on - strand, within metC at 3912.298 kb on - strand, within metC at 3912.476 kb on - strand at 3912.484 kb on - strand at 3912.509 kb on + strand at 3912.542 kb on + strand at 3912.655 kb on + strand, within LU632_RS20550 at 3912.655 kb on + strand, within LU632_RS20550 at 3912.656 kb on - strand, within LU632_RS20550 at 3912.656 kb on - strand, within LU632_RS20550 at 3912.657 kb on + strand, within LU632_RS20550 at 3912.657 kb on + strand, within LU632_RS20550 at 3912.658 kb on - strand, within LU632_RS20550 at 3912.658 kb on - strand, within LU632_RS20550 at 3912.674 kb on + strand, within LU632_RS20550 at 3912.675 kb on - strand, within LU632_RS20550 at 3912.675 kb on - strand, within LU632_RS20550 at 3912.709 kb on + strand, within LU632_RS20550 at 3912.797 kb on + strand, within LU632_RS20550 at 3912.866 kb on + strand, within LU632_RS20550 at 3913.125 kb on - strand, within LU632_RS20550 at 3913.174 kb on + strand at 3913.178 kb on - strand at 3913.237 kb on - strand at 3913.400 kb on + strand at 3913.437 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 3,910,298 + -1.0 3,910,299 - -1.8 3,910,362 + +0.2 3,910,363 - -0.2 3,910,469 + exbB LU632_RS20540 0.21 -0.9 3,910,470 - exbB LU632_RS20540 0.21 +1.4 3,910,486 + exbB LU632_RS20540 0.23 +0.4 3,910,579 - exbB LU632_RS20540 0.36 +1.6 3,910,606 + exbB LU632_RS20540 0.39 -0.4 3,911,006 + -0.3 3,911,006 + -0.5 3,911,006 + +0.4 3,911,007 - -0.0 3,911,007 - -0.2 3,911,207 + +0.3 3,911,207 + +0.4 3,911,447 - metC LU632_RS20545 0.13 -2.0 3,911,451 + metC LU632_RS20545 0.14 -1.4 3,911,451 + metC LU632_RS20545 0.14 -0.6 3,911,615 - metC LU632_RS20545 0.27 -1.6 3,911,650 + metC LU632_RS20545 0.30 -2.6 3,911,881 - metC LU632_RS20545 0.50 -0.2 3,911,891 + metC LU632_RS20545 0.50 -1.8 3,911,891 + metC LU632_RS20545 0.50 -1.6 3,911,891 + metC LU632_RS20545 0.50 -1.8 3,911,912 + metC LU632_RS20545 0.52 -0.7 3,911,912 + metC LU632_RS20545 0.52 +0.0 3,911,984 - metC LU632_RS20545 0.58 -2.8 3,911,997 + metC LU632_RS20545 0.59 -1.8 3,911,997 + metC LU632_RS20545 0.59 -2.0 3,911,998 - metC LU632_RS20545 0.59 -2.8 3,912,003 - metC LU632_RS20545 0.60 -0.2 3,912,033 + metC LU632_RS20545 0.62 +0.8 3,912,033 + metC LU632_RS20545 0.62 +0.8 3,912,033 + metC LU632_RS20545 0.62 -1.2 3,912,034 - metC LU632_RS20545 0.62 +0.8 3,912,034 - metC LU632_RS20545 0.62 -3.2 3,912,034 - metC LU632_RS20545 0.62 -1.2 3,912,068 - metC LU632_RS20545 0.65 -0.8 3,912,158 - metC LU632_RS20545 0.73 -0.8 3,912,288 - metC LU632_RS20545 0.83 -2.4 3,912,298 - metC LU632_RS20545 0.84 -2.6 3,912,476 - -2.2 3,912,484 - +0.2 3,912,509 + +0.1 3,912,542 + -0.6 3,912,655 + LU632_RS20550 0.11 -1.7 3,912,655 + LU632_RS20550 0.11 -0.2 3,912,656 - LU632_RS20550 0.11 -1.8 3,912,656 - LU632_RS20550 0.11 -1.8 3,912,657 + LU632_RS20550 0.11 -0.9 3,912,657 + LU632_RS20550 0.11 -0.7 3,912,658 - LU632_RS20550 0.12 +0.0 3,912,658 - LU632_RS20550 0.12 -1.6 3,912,674 + LU632_RS20550 0.14 -1.2 3,912,675 - LU632_RS20550 0.14 -0.8 3,912,675 - LU632_RS20550 0.14 -1.6 3,912,709 + LU632_RS20550 0.19 -1.6 3,912,797 + LU632_RS20550 0.33 +0.6 3,912,866 + LU632_RS20550 0.43 -0.8 3,913,125 - LU632_RS20550 0.83 -1.3 3,913,174 + -1.8 3,913,178 - -0.7 3,913,237 - +1.2 3,913,400 + +0.3 3,913,437 - +0.8
Or see this region's nucleotide sequence