Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gluQRS and pcnB are separated by 106 nucleotides pcnB and folK overlap by 4 nucleotides folK and panB are separated by 60 nucleotides
LU632_RS20085: gluQRS - tRNA glutamyl-Q(34) synthetase GluQRS, at 3,826,027 to 3,826,932
gluQRS
LU632_RS20090: pcnB - polynucleotide adenylyltransferase PcnB, at 3,827,039 to 3,828,454
pcnB
LU632_RS20095: folK - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 3,828,451 to 3,828,930
folK
LU632_RS20100: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,828,991 to 3,829,785
panB
Position (kb)
3827
3828
3829 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3826.039 kb on + strand at 3826.039 kb on + strand at 3826.039 kb on + strand at 3826.039 kb on + strand at 3826.040 kb on - strand at 3826.040 kb on - strand at 3826.041 kb on + strand at 3826.111 kb on + strand at 3826.112 kb on - strand at 3826.112 kb on - strand at 3826.112 kb on - strand at 3826.318 kb on + strand, within gluQRS at 3826.318 kb on + strand, within gluQRS at 3826.388 kb on + strand, within gluQRS at 3826.389 kb on - strand, within gluQRS at 3826.389 kb on - strand, within gluQRS at 3826.537 kb on + strand, within gluQRS at 3826.537 kb on + strand, within gluQRS at 3826.538 kb on - strand, within gluQRS at 3826.540 kb on - strand, within gluQRS at 3826.586 kb on + strand, within gluQRS at 3826.636 kb on + strand, within gluQRS at 3826.637 kb on - strand, within gluQRS at 3826.666 kb on + strand, within gluQRS at 3826.677 kb on - strand, within gluQRS at 3826.817 kb on + strand, within gluQRS at 3826.817 kb on + strand, within gluQRS at 3826.850 kb on + strand at 3826.850 kb on + strand at 3826.899 kb on + strand at 3826.899 kb on + strand at 3826.983 kb on + strand at 3826.984 kb on - strand at 3827.549 kb on + strand, within pcnB at 3827.552 kb on + strand, within pcnB at 3827.552 kb on + strand, within pcnB at 3827.893 kb on + strand, within pcnB at 3828.216 kb on + strand, within pcnB at 3828.958 kb on + strand at 3828.958 kb on + strand at 3828.958 kb on + strand at 3828.959 kb on - strand at 3828.974 kb on + strand at 3829.063 kb on + strand at 3829.072 kb on - strand, within panB at 3829.189 kb on + strand, within panB at 3829.189 kb on + strand, within panB at 3829.259 kb on - strand, within panB at 3829.300 kb on - strand, within panB at 3829.317 kb on + strand, within panB at 3829.318 kb on - strand, within panB
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 3,826,039 + -0.5 3,826,039 + -1.2 3,826,039 + -0.2 3,826,039 + +0.6 3,826,040 - +0.1 3,826,040 - -0.2 3,826,041 + +0.4 3,826,111 + +0.5 3,826,112 - +0.6 3,826,112 - +0.4 3,826,112 - +1.4 3,826,318 + gluQRS LU632_RS20085 0.32 -0.2 3,826,318 + gluQRS LU632_RS20085 0.32 +1.8 3,826,388 + gluQRS LU632_RS20085 0.40 -0.5 3,826,389 - gluQRS LU632_RS20085 0.40 +0.2 3,826,389 - gluQRS LU632_RS20085 0.40 -1.2 3,826,537 + gluQRS LU632_RS20085 0.56 +0.3 3,826,537 + gluQRS LU632_RS20085 0.56 -0.6 3,826,538 - gluQRS LU632_RS20085 0.56 +0.3 3,826,540 - gluQRS LU632_RS20085 0.57 -0.2 3,826,586 + gluQRS LU632_RS20085 0.62 +0.2 3,826,636 + gluQRS LU632_RS20085 0.67 +0.8 3,826,637 - gluQRS LU632_RS20085 0.67 +0.1 3,826,666 + gluQRS LU632_RS20085 0.71 -0.3 3,826,677 - gluQRS LU632_RS20085 0.72 +0.4 3,826,817 + gluQRS LU632_RS20085 0.87 +1.3 3,826,817 + gluQRS LU632_RS20085 0.87 +0.8 3,826,850 + +0.4 3,826,850 + -0.0 3,826,899 + +0.5 3,826,899 + +0.1 3,826,983 + -2.1 3,826,984 - -1.2 3,827,549 + pcnB LU632_RS20090 0.36 -0.8 3,827,552 + pcnB LU632_RS20090 0.36 -1.8 3,827,552 + pcnB LU632_RS20090 0.36 -1.5 3,827,893 + pcnB LU632_RS20090 0.60 -1.8 3,828,216 + pcnB LU632_RS20090 0.83 +0.4 3,828,958 + +0.8 3,828,958 + +0.3 3,828,958 + +1.6 3,828,959 - +0.8 3,828,974 + -0.0 3,829,063 + +0.6 3,829,072 - panB LU632_RS20100 0.10 +0.4 3,829,189 + panB LU632_RS20100 0.25 -0.2 3,829,189 + panB LU632_RS20100 0.25 -0.2 3,829,259 - panB LU632_RS20100 0.34 +2.6 3,829,300 - panB LU632_RS20100 0.39 -0.6 3,829,317 + panB LU632_RS20100 0.41 +1.4 3,829,318 - panB LU632_RS20100 0.41 -0.8
Or see this region's nucleotide sequence