Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pitA and uspB are separated by 65 nucleotides uspB and uspA are separated by 220 nucleotides uspA and rsmJ are separated by 205 nucleotides rsmJ and prlC overlap by 4 nucleotides
LU632_RS19450: pitA - inorganic phosphate transporter PitA, at 3,683,857 to 3,685,356
pitA
LU632_RS19455: uspB - universal stress protein UspB, at 3,685,422 to 3,685,759
uspB
LU632_RS19460: uspA - universal stress protein UspA, at 3,685,980 to 3,686,414
uspA
LU632_RS19465: rsmJ - 16S rRNA (guanine(1516)-N(2))-methyltransferase RsmJ, at 3,686,620 to 3,687,375
rsmJ
LU632_RS19470: prlC - oligopeptidase A, at 3,687,372 to 3,689,420
prlC
Position (kb)
3685
3686
3687 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3685.014 kb on + strand, within pitA at 3685.114 kb on + strand, within pitA at 3685.172 kb on - strand, within pitA at 3685.203 kb on - strand, within pitA at 3685.313 kb on + strand at 3685.355 kb on - strand at 3685.378 kb on - strand at 3685.562 kb on + strand, within uspB at 3685.563 kb on - strand, within uspB at 3685.563 kb on - strand, within uspB at 3685.564 kb on + strand, within uspB at 3685.564 kb on + strand, within uspB at 3685.565 kb on - strand, within uspB at 3685.565 kb on - strand, within uspB at 3685.626 kb on + strand, within uspB at 3685.626 kb on + strand, within uspB at 3685.627 kb on - strand, within uspB at 3685.627 kb on - strand, within uspB at 3685.627 kb on - strand, within uspB at 3685.627 kb on - strand, within uspB at 3685.630 kb on + strand, within uspB at 3685.662 kb on + strand, within uspB at 3685.663 kb on - strand, within uspB at 3685.668 kb on + strand, within uspB at 3685.725 kb on + strand, within uspB at 3685.725 kb on + strand, within uspB at 3685.726 kb on - strand at 3685.726 kb on - strand at 3685.726 kb on - strand at 3685.726 kb on - strand at 3685.759 kb on + strand at 3685.760 kb on - strand at 3685.789 kb on - strand at 3685.934 kb on + strand at 3685.965 kb on - strand at 3685.980 kb on - strand at 3685.980 kb on - strand at 3685.980 kb on - strand at 3685.986 kb on + strand at 3685.986 kb on + strand at 3685.987 kb on - strand at 3685.988 kb on + strand at 3685.988 kb on + strand at 3686.027 kb on + strand, within uspA at 3686.067 kb on + strand, within uspA at 3686.106 kb on + strand, within uspA at 3686.150 kb on + strand, within uspA at 3686.283 kb on + strand, within uspA at 3686.290 kb on + strand, within uspA at 3686.291 kb on - strand, within uspA at 3686.291 kb on - strand, within uspA at 3686.369 kb on - strand, within uspA at 3686.378 kb on + strand at 3686.378 kb on + strand at 3686.413 kb on - strand at 3686.667 kb on + strand at 3686.855 kb on + strand, within rsmJ at 3686.855 kb on + strand, within rsmJ at 3686.856 kb on - strand, within rsmJ at 3686.886 kb on + strand, within rsmJ at 3686.886 kb on + strand, within rsmJ at 3686.887 kb on - strand, within rsmJ at 3686.906 kb on - strand, within rsmJ at 3687.113 kb on + strand, within rsmJ at 3687.114 kb on - strand, within rsmJ at 3687.360 kb on + strand at 3687.361 kb on - strand at 3687.397 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 3,685,014 + pitA LU632_RS19450 0.77 -0.2 3,685,114 + pitA LU632_RS19450 0.84 -0.2 3,685,172 - pitA LU632_RS19450 0.88 -1.2 3,685,203 - pitA LU632_RS19450 0.90 -0.2 3,685,313 + -0.2 3,685,355 - -0.2 3,685,378 - +0.5 3,685,562 + uspB LU632_RS19455 0.41 +1.0 3,685,563 - uspB LU632_RS19455 0.42 +1.4 3,685,563 - uspB LU632_RS19455 0.42 +1.4 3,685,564 + uspB LU632_RS19455 0.42 +1.8 3,685,564 + uspB LU632_RS19455 0.42 +1.1 3,685,565 - uspB LU632_RS19455 0.42 -0.2 3,685,565 - uspB LU632_RS19455 0.42 -0.2 3,685,626 + uspB LU632_RS19455 0.60 -1.4 3,685,626 + uspB LU632_RS19455 0.60 +0.3 3,685,627 - uspB LU632_RS19455 0.61 +0.4 3,685,627 - uspB LU632_RS19455 0.61 -1.2 3,685,627 - uspB LU632_RS19455 0.61 +0.8 3,685,627 - uspB LU632_RS19455 0.61 -0.4 3,685,630 + uspB LU632_RS19455 0.62 +0.1 3,685,662 + uspB LU632_RS19455 0.71 +0.6 3,685,663 - uspB LU632_RS19455 0.71 -0.2 3,685,668 + uspB LU632_RS19455 0.73 +1.8 3,685,725 + uspB LU632_RS19455 0.90 +0.8 3,685,725 + uspB LU632_RS19455 0.90 +0.8 3,685,726 - -0.7 3,685,726 - +0.1 3,685,726 - +0.5 3,685,726 - -0.2 3,685,759 + -0.2 3,685,760 - +1.3 3,685,789 - +0.9 3,685,934 + -1.2 3,685,965 - -1.8 3,685,980 - -0.2 3,685,980 - +0.2 3,685,980 - -1.2 3,685,986 + -0.2 3,685,986 + +0.8 3,685,987 - -1.1 3,685,988 + -1.8 3,685,988 + -1.2 3,686,027 + uspA LU632_RS19460 0.11 -2.2 3,686,067 + uspA LU632_RS19460 0.20 +0.2 3,686,106 + uspA LU632_RS19460 0.29 -1.2 3,686,150 + uspA LU632_RS19460 0.39 -1.2 3,686,283 + uspA LU632_RS19460 0.70 +0.2 3,686,290 + uspA LU632_RS19460 0.71 -1.2 3,686,291 - uspA LU632_RS19460 0.71 +0.8 3,686,291 - uspA LU632_RS19460 0.71 -2.2 3,686,369 - uspA LU632_RS19460 0.89 -1.2 3,686,378 + -1.3 3,686,378 + -2.2 3,686,413 - -0.5 3,686,667 + -0.7 3,686,855 + rsmJ LU632_RS19465 0.31 +0.8 3,686,855 + rsmJ LU632_RS19465 0.31 -0.7 3,686,856 - rsmJ LU632_RS19465 0.31 +0.0 3,686,886 + rsmJ LU632_RS19465 0.35 -0.1 3,686,886 + rsmJ LU632_RS19465 0.35 +0.1 3,686,887 - rsmJ LU632_RS19465 0.35 -1.2 3,686,906 - rsmJ LU632_RS19465 0.38 -0.2 3,687,113 + rsmJ LU632_RS19465 0.65 -1.2 3,687,114 - rsmJ LU632_RS19465 0.65 -0.8 3,687,360 + -0.3 3,687,361 - +0.5 3,687,397 + +1.4
Or see this region's nucleotide sequence