Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phoR and phoB are separated by 24 nucleotides phoB and sbcD are separated by 200 nucleotides
LU632_RS18920: phoR - phosphate regulon sensor histidine kinase PhoR, at 3,569,521 to 3,570,819
phoR
LU632_RS18925: phoB - phosphate response regulator transcription factor PhoB, at 3,570,844 to 3,571,533
phoB
LU632_RS18930: sbcD - exonuclease subunit SbcD, at 3,571,734 to 3,572,957
sbcD
Position (kb)
3570
3571
3572 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3569.898 kb on + strand, within phoR at 3569.898 kb on + strand, within phoR at 3569.899 kb on - strand, within phoR at 3570.088 kb on - strand, within phoR at 3570.261 kb on - strand, within phoR at 3570.451 kb on - strand, within phoR at 3570.463 kb on - strand, within phoR at 3570.564 kb on + strand, within phoR at 3570.633 kb on + strand, within phoR at 3570.675 kb on + strand, within phoR at 3570.675 kb on + strand, within phoR at 3570.827 kb on - strand at 3570.925 kb on - strand, within phoB at 3570.939 kb on + strand, within phoB at 3570.940 kb on - strand, within phoB at 3570.968 kb on + strand, within phoB at 3570.985 kb on + strand, within phoB at 3570.986 kb on - strand, within phoB at 3570.986 kb on - strand, within phoB at 3570.986 kb on - strand, within phoB at 3570.993 kb on + strand, within phoB at 3571.013 kb on + strand, within phoB at 3571.013 kb on + strand, within phoB at 3571.014 kb on - strand, within phoB at 3571.053 kb on + strand, within phoB at 3571.053 kb on + strand, within phoB at 3571.054 kb on - strand, within phoB at 3571.054 kb on - strand, within phoB at 3571.055 kb on + strand, within phoB at 3571.056 kb on - strand, within phoB at 3571.056 kb on - strand, within phoB at 3571.168 kb on - strand, within phoB at 3571.221 kb on + strand, within phoB at 3571.221 kb on + strand, within phoB at 3571.229 kb on + strand, within phoB at 3571.229 kb on + strand, within phoB at 3571.229 kb on + strand, within phoB at 3571.230 kb on - strand, within phoB at 3571.230 kb on - strand, within phoB at 3571.305 kb on + strand, within phoB at 3571.338 kb on + strand, within phoB at 3571.338 kb on + strand, within phoB at 3571.338 kb on + strand, within phoB at 3571.521 kb on + strand at 3571.535 kb on - strand at 3571.583 kb on + strand at 3571.584 kb on - strand at 3571.698 kb on + strand at 3571.699 kb on - strand at 3571.781 kb on + strand at 3571.905 kb on + strand, within sbcD at 3572.080 kb on + strand, within sbcD at 3572.080 kb on + strand, within sbcD at 3572.081 kb on - strand, within sbcD at 3572.081 kb on - strand, within sbcD at 3572.157 kb on - strand, within sbcD at 3572.160 kb on - strand, within sbcD at 3572.226 kb on + strand, within sbcD at 3572.349 kb on + strand, within sbcD at 3572.350 kb on - strand, within sbcD at 3572.350 kb on - strand, within sbcD at 3572.417 kb on + strand, within sbcD at 3572.418 kb on - strand, within sbcD at 3572.454 kb on - strand, within sbcD at 3572.454 kb on - strand, within sbcD
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 3,569,898 + phoR LU632_RS18920 0.29 +0.7 3,569,898 + phoR LU632_RS18920 0.29 -0.8 3,569,899 - phoR LU632_RS18920 0.29 -0.2 3,570,088 - phoR LU632_RS18920 0.44 +1.4 3,570,261 - phoR LU632_RS18920 0.57 +0.1 3,570,451 - phoR LU632_RS18920 0.72 +1.0 3,570,463 - phoR LU632_RS18920 0.73 +0.6 3,570,564 + phoR LU632_RS18920 0.80 +0.7 3,570,633 + phoR LU632_RS18920 0.86 +0.4 3,570,675 + phoR LU632_RS18920 0.89 -0.1 3,570,675 + phoR LU632_RS18920 0.89 +1.4 3,570,827 - -0.5 3,570,925 - phoB LU632_RS18925 0.12 -1.5 3,570,939 + phoB LU632_RS18925 0.14 +2.6 3,570,940 - phoB LU632_RS18925 0.14 -0.0 3,570,968 + phoB LU632_RS18925 0.18 +0.8 3,570,985 + phoB LU632_RS18925 0.20 +0.8 3,570,986 - phoB LU632_RS18925 0.21 -0.5 3,570,986 - phoB LU632_RS18925 0.21 +1.7 3,570,986 - phoB LU632_RS18925 0.21 -0.8 3,570,993 + phoB LU632_RS18925 0.22 +0.1 3,571,013 + phoB LU632_RS18925 0.24 -0.8 3,571,013 + phoB LU632_RS18925 0.24 -0.5 3,571,014 - phoB LU632_RS18925 0.25 +0.2 3,571,053 + phoB LU632_RS18925 0.30 +0.2 3,571,053 + phoB LU632_RS18925 0.30 -0.1 3,571,054 - phoB LU632_RS18925 0.30 -0.2 3,571,054 - phoB LU632_RS18925 0.30 +0.8 3,571,055 + phoB LU632_RS18925 0.31 -0.2 3,571,056 - phoB LU632_RS18925 0.31 +0.4 3,571,056 - phoB LU632_RS18925 0.31 -0.9 3,571,168 - phoB LU632_RS18925 0.47 -0.2 3,571,221 + phoB LU632_RS18925 0.55 -0.2 3,571,221 + phoB LU632_RS18925 0.55 +2.8 3,571,229 + phoB LU632_RS18925 0.56 +0.2 3,571,229 + phoB LU632_RS18925 0.56 -2.0 3,571,229 + phoB LU632_RS18925 0.56 -1.0 3,571,230 - phoB LU632_RS18925 0.56 +0.8 3,571,230 - phoB LU632_RS18925 0.56 -0.1 3,571,305 + phoB LU632_RS18925 0.67 -1.5 3,571,338 + phoB LU632_RS18925 0.72 -1.8 3,571,338 + phoB LU632_RS18925 0.72 -0.2 3,571,338 + phoB LU632_RS18925 0.72 +0.8 3,571,521 + +0.9 3,571,535 - -0.0 3,571,583 + +1.2 3,571,584 - -0.2 3,571,698 + +2.8 3,571,699 - -0.9 3,571,781 + +0.4 3,571,905 + sbcD LU632_RS18930 0.14 -0.6 3,572,080 + sbcD LU632_RS18930 0.28 -0.5 3,572,080 + sbcD LU632_RS18930 0.28 -0.4 3,572,081 - sbcD LU632_RS18930 0.28 -0.2 3,572,081 - sbcD LU632_RS18930 0.28 -1.2 3,572,157 - sbcD LU632_RS18930 0.35 -1.2 3,572,160 - sbcD LU632_RS18930 0.35 +1.1 3,572,226 + sbcD LU632_RS18930 0.40 +1.0 3,572,349 + sbcD LU632_RS18930 0.50 -0.2 3,572,350 - sbcD LU632_RS18930 0.50 -0.6 3,572,350 - sbcD LU632_RS18930 0.50 -0.2 3,572,417 + sbcD LU632_RS18930 0.56 -2.8 3,572,418 - sbcD LU632_RS18930 0.56 -0.2 3,572,454 - sbcD LU632_RS18930 0.59 -1.2 3,572,454 - sbcD LU632_RS18930 0.59 +0.8
Or see this region's nucleotide sequence