Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS18915 and phoR are separated by 834 nucleotides phoR and phoB are separated by 24 nucleotides phoB and sbcD are separated by 200 nucleotides
LU632_RS18915: LU632_RS18915 - IS1 family transposase, at 3,568,052 to 3,568,686
_RS18915
LU632_RS18920: phoR - phosphate regulon sensor histidine kinase PhoR, at 3,569,521 to 3,570,819
phoR
LU632_RS18925: phoB - phosphate response regulator transcription factor PhoB, at 3,570,844 to 3,571,533
phoB
LU632_RS18930: sbcD - exonuclease subunit SbcD, at 3,571,734 to 3,572,957
sbcD
Position (kb)
3569
3570
3571 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3568.719 kb on - strand at 3568.745 kb on + strand at 3568.759 kb on + strand at 3568.760 kb on - strand at 3568.776 kb on + strand at 3568.776 kb on + strand at 3568.777 kb on - strand at 3568.824 kb on - strand at 3568.848 kb on + strand at 3568.848 kb on + strand at 3568.873 kb on + strand at 3568.893 kb on - strand at 3568.897 kb on - strand at 3568.901 kb on + strand at 3568.936 kb on - strand at 3568.937 kb on - strand at 3568.961 kb on - strand at 3568.977 kb on - strand at 3569.019 kb on - strand at 3569.019 kb on - strand at 3569.044 kb on + strand at 3569.059 kb on + strand at 3569.227 kb on + strand at 3569.228 kb on - strand at 3569.228 kb on - strand at 3569.228 kb on - strand at 3569.270 kb on + strand at 3569.301 kb on + strand at 3569.301 kb on + strand at 3569.309 kb on - strand at 3569.316 kb on - strand at 3569.323 kb on - strand at 3569.337 kb on - strand at 3569.337 kb on - strand at 3569.477 kb on + strand at 3569.481 kb on + strand at 3569.562 kb on + strand at 3569.569 kb on - strand at 3569.634 kb on - strand at 3569.638 kb on + strand at 3569.639 kb on - strand at 3569.725 kb on - strand, within phoR at 3569.759 kb on - strand, within phoR at 3569.841 kb on - strand, within phoR at 3569.898 kb on + strand, within phoR at 3569.898 kb on + strand, within phoR at 3569.899 kb on - strand, within phoR at 3570.088 kb on - strand, within phoR at 3570.261 kb on - strand, within phoR at 3570.451 kb on - strand, within phoR at 3570.463 kb on - strand, within phoR at 3570.564 kb on + strand, within phoR at 3570.633 kb on + strand, within phoR at 3570.675 kb on + strand, within phoR at 3570.675 kb on + strand, within phoR at 3570.827 kb on - strand at 3570.925 kb on - strand, within phoB at 3570.939 kb on + strand, within phoB at 3570.940 kb on - strand, within phoB at 3570.968 kb on + strand, within phoB at 3570.985 kb on + strand, within phoB at 3570.986 kb on - strand, within phoB at 3570.986 kb on - strand, within phoB at 3570.986 kb on - strand, within phoB at 3570.993 kb on + strand, within phoB at 3571.013 kb on + strand, within phoB at 3571.013 kb on + strand, within phoB at 3571.014 kb on - strand, within phoB at 3571.053 kb on + strand, within phoB at 3571.053 kb on + strand, within phoB at 3571.054 kb on - strand, within phoB at 3571.054 kb on - strand, within phoB at 3571.055 kb on + strand, within phoB at 3571.056 kb on - strand, within phoB at 3571.056 kb on - strand, within phoB at 3571.168 kb on - strand, within phoB at 3571.221 kb on + strand, within phoB at 3571.221 kb on + strand, within phoB at 3571.229 kb on + strand, within phoB at 3571.229 kb on + strand, within phoB at 3571.229 kb on + strand, within phoB at 3571.230 kb on - strand, within phoB at 3571.230 kb on - strand, within phoB at 3571.305 kb on + strand, within phoB at 3571.338 kb on + strand, within phoB at 3571.338 kb on + strand, within phoB at 3571.338 kb on + strand, within phoB at 3571.521 kb on + strand at 3571.535 kb on - strand at 3571.583 kb on + strand at 3571.584 kb on - strand at 3571.698 kb on + strand at 3571.699 kb on - strand at 3571.781 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 3,568,719 - +0.2 3,568,745 + +0.7 3,568,759 + -0.8 3,568,760 - +0.2 3,568,776 + -0.1 3,568,776 + -0.4 3,568,777 - -0.1 3,568,824 - -0.2 3,568,848 + +0.8 3,568,848 + -0.1 3,568,873 + +0.5 3,568,893 - +0.0 3,568,897 - +1.7 3,568,901 + -0.6 3,568,936 - +0.7 3,568,937 - +1.1 3,568,961 - -0.6 3,568,977 - +0.4 3,569,019 - -0.5 3,569,019 - +2.1 3,569,044 + -2.1 3,569,059 + +0.2 3,569,227 + -0.6 3,569,228 - -0.0 3,569,228 - +1.8 3,569,228 - -0.5 3,569,270 + -0.1 3,569,301 + -0.5 3,569,301 + +0.4 3,569,309 - -1.4 3,569,316 - +1.4 3,569,323 - -1.8 3,569,337 - +0.7 3,569,337 - -1.2 3,569,477 + +1.8 3,569,481 + -0.8 3,569,562 + -0.4 3,569,569 - +1.4 3,569,634 - -0.2 3,569,638 + +0.5 3,569,639 - +0.3 3,569,725 - phoR LU632_RS18920 0.16 +0.1 3,569,759 - phoR LU632_RS18920 0.18 +0.4 3,569,841 - phoR LU632_RS18920 0.25 +1.4 3,569,898 + phoR LU632_RS18920 0.29 +0.7 3,569,898 + phoR LU632_RS18920 0.29 -0.8 3,569,899 - phoR LU632_RS18920 0.29 -0.2 3,570,088 - phoR LU632_RS18920 0.44 +1.4 3,570,261 - phoR LU632_RS18920 0.57 +0.1 3,570,451 - phoR LU632_RS18920 0.72 +1.0 3,570,463 - phoR LU632_RS18920 0.73 +0.6 3,570,564 + phoR LU632_RS18920 0.80 +0.7 3,570,633 + phoR LU632_RS18920 0.86 +0.4 3,570,675 + phoR LU632_RS18920 0.89 -0.1 3,570,675 + phoR LU632_RS18920 0.89 +1.4 3,570,827 - -0.5 3,570,925 - phoB LU632_RS18925 0.12 -1.5 3,570,939 + phoB LU632_RS18925 0.14 +2.6 3,570,940 - phoB LU632_RS18925 0.14 -0.0 3,570,968 + phoB LU632_RS18925 0.18 +0.8 3,570,985 + phoB LU632_RS18925 0.20 +0.8 3,570,986 - phoB LU632_RS18925 0.21 -0.5 3,570,986 - phoB LU632_RS18925 0.21 +1.7 3,570,986 - phoB LU632_RS18925 0.21 -0.8 3,570,993 + phoB LU632_RS18925 0.22 +0.1 3,571,013 + phoB LU632_RS18925 0.24 -0.8 3,571,013 + phoB LU632_RS18925 0.24 -0.5 3,571,014 - phoB LU632_RS18925 0.25 +0.2 3,571,053 + phoB LU632_RS18925 0.30 +0.2 3,571,053 + phoB LU632_RS18925 0.30 -0.1 3,571,054 - phoB LU632_RS18925 0.30 -0.2 3,571,054 - phoB LU632_RS18925 0.30 +0.8 3,571,055 + phoB LU632_RS18925 0.31 -0.2 3,571,056 - phoB LU632_RS18925 0.31 +0.4 3,571,056 - phoB LU632_RS18925 0.31 -0.9 3,571,168 - phoB LU632_RS18925 0.47 -0.2 3,571,221 + phoB LU632_RS18925 0.55 -0.2 3,571,221 + phoB LU632_RS18925 0.55 +2.8 3,571,229 + phoB LU632_RS18925 0.56 +0.2 3,571,229 + phoB LU632_RS18925 0.56 -2.0 3,571,229 + phoB LU632_RS18925 0.56 -1.0 3,571,230 - phoB LU632_RS18925 0.56 +0.8 3,571,230 - phoB LU632_RS18925 0.56 -0.1 3,571,305 + phoB LU632_RS18925 0.67 -1.5 3,571,338 + phoB LU632_RS18925 0.72 -1.8 3,571,338 + phoB LU632_RS18925 0.72 -0.2 3,571,338 + phoB LU632_RS18925 0.72 +0.8 3,571,521 + +0.9 3,571,535 - -0.0 3,571,583 + +1.2 3,571,584 - -0.2 3,571,698 + +2.8 3,571,699 - -0.9 3,571,781 + +0.4
Or see this region's nucleotide sequence