Strain Fitness in Erwinia tracheiphila SCR3 around LU632_RS18835

Experiment: survival 4C, 24 hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdxs and ispA are separated by 19 nucleotidesispA and xseB overlap by 1 nucleotidesxseB and thiI are separated by 329 nucleotides LU632_RS18830: dxs - 1-deoxy-D-xylulose-5-phosphate synthase, at 3,551,197 to 3,553,062 dxs LU632_RS18835: ispA - (2E,6E)-farnesyl diphosphate synthase, at 3,553,082 to 3,553,981 ispA LU632_RS18840: xseB - exodeoxyribonuclease VII small subunit, at 3,553,981 to 3,554,223 xseB LU632_RS18845: thiI - tRNA 4-thiouridine(8) synthase ThiI, at 3,554,553 to 3,556,001 thiI Position (kb) 3553 3554Strain fitness (log2 ratio) -3 -2 -1 0 1at 3553.084 kb on - strandat 3554.224 kb on - strandat 3554.343 kb on + strandat 3554.361 kb on + strandat 3554.368 kb on + strandat 3554.383 kb on + strandat 3554.383 kb on + strandat 3554.384 kb on - strandat 3554.405 kb on + strandat 3554.423 kb on + strandat 3554.424 kb on - strandat 3554.428 kb on - strandat 3554.453 kb on - strandat 3554.564 kb on + strandat 3554.592 kb on - strandat 3554.661 kb on + strandat 3554.662 kb on - strandat 3554.735 kb on + strand, within thiIat 3554.736 kb on - strand, within thiIat 3554.740 kb on + strand, within thiIat 3554.740 kb on + strand, within thiIat 3554.740 kb on + strand, within thiIat 3554.740 kb on + strand, within thiIat 3554.740 kb on + strand, within thiIat 3554.741 kb on - strand, within thiIat 3554.802 kb on + strand, within thiIat 3554.803 kb on - strand, within thiIat 3554.884 kb on + strand, within thiIat 3554.903 kb on + strand, within thiIat 3554.931 kb on + strand, within thiIat 3554.931 kb on + strand, within thiI

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Per-strain Table

Position Strand Gene LocusTag Fraction survival 4C, 24 hrs
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3,553,084 - -2.9
3,554,224 - +1.4
3,554,343 + -0.6
3,554,361 + -0.6
3,554,368 + -2.2
3,554,383 + -0.8
3,554,383 + +0.8
3,554,384 - -1.6
3,554,405 + -0.2
3,554,423 + -1.5
3,554,424 - +0.1
3,554,428 - -0.2
3,554,453 - +1.4
3,554,564 + -0.1
3,554,592 - -0.8
3,554,661 + -1.6
3,554,662 - +0.1
3,554,735 + thiI LU632_RS18845 0.13 +0.4
3,554,736 - thiI LU632_RS18845 0.13 +0.2
3,554,740 + thiI LU632_RS18845 0.13 -1.0
3,554,740 + thiI LU632_RS18845 0.13 -0.2
3,554,740 + thiI LU632_RS18845 0.13 +0.8
3,554,740 + thiI LU632_RS18845 0.13 -0.6
3,554,740 + thiI LU632_RS18845 0.13 -1.2
3,554,741 - thiI LU632_RS18845 0.13 -0.8
3,554,802 + thiI LU632_RS18845 0.17 +0.2
3,554,803 - thiI LU632_RS18845 0.17 +0.1
3,554,884 + thiI LU632_RS18845 0.23 +0.6
3,554,903 + thiI LU632_RS18845 0.24 +0.4
3,554,931 + thiI LU632_RS18845 0.26 -1.4
3,554,931 + thiI LU632_RS18845 0.26 -0.2

Or see this region's nucleotide sequence