Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fruA and fruK are separated by 15 nucleotides fruK and fruB overlap by 4 nucleotides
LU632_RS17315: fruA - PTS fructose transporter subunit IIBC, at 3,254,161 to 3,255,861
fruA
LU632_RS17320: fruK - 1-phosphofructokinase, at 3,255,877 to 3,256,815
fruK
LU632_RS17325: fruB - fused PTS fructose transporter subunit IIA/HPr protein, at 3,256,812 to 3,257,945
fruB
Position (kb)
3255
3256
3257 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3255.070 kb on - strand, within fruA at 3255.070 kb on - strand, within fruA at 3255.140 kb on + strand, within fruA at 3255.140 kb on + strand, within fruA at 3255.141 kb on - strand, within fruA at 3255.213 kb on + strand, within fruA at 3255.297 kb on + strand, within fruA at 3255.308 kb on + strand, within fruA at 3255.308 kb on + strand, within fruA at 3255.319 kb on - strand, within fruA at 3255.348 kb on + strand, within fruA at 3255.348 kb on + strand, within fruA at 3255.349 kb on - strand, within fruA at 3255.364 kb on - strand, within fruA at 3255.496 kb on - strand, within fruA at 3255.496 kb on - strand, within fruA at 3255.496 kb on - strand, within fruA at 3255.652 kb on + strand, within fruA at 3255.653 kb on - strand, within fruA at 3255.653 kb on - strand, within fruA at 3255.706 kb on - strand at 3255.711 kb on + strand at 3255.711 kb on + strand at 3255.711 kb on + strand at 3255.712 kb on - strand at 3255.799 kb on - strand at 3255.844 kb on + strand at 3255.845 kb on - strand at 3255.862 kb on + strand at 3255.862 kb on + strand at 3255.863 kb on - strand at 3255.863 kb on - strand at 3255.882 kb on + strand at 3255.892 kb on + strand at 3255.892 kb on + strand at 3255.892 kb on + strand at 3256.028 kb on + strand, within fruK at 3256.201 kb on + strand, within fruK at 3256.201 kb on + strand, within fruK at 3256.202 kb on - strand, within fruK at 3256.323 kb on + strand, within fruK at 3256.324 kb on - strand, within fruK at 3256.548 kb on + strand, within fruK at 3256.549 kb on - strand, within fruK at 3257.016 kb on - strand, within fruB at 3257.179 kb on + strand, within fruB at 3257.179 kb on + strand, within fruB at 3257.179 kb on + strand, within fruB at 3257.180 kb on - strand, within fruB at 3257.180 kb on - strand, within fruB at 3257.182 kb on - strand, within fruB at 3257.198 kb on - strand, within fruB at 3257.198 kb on - strand, within fruB at 3257.477 kb on + strand, within fruB at 3257.606 kb on + strand, within fruB at 3257.607 kb on - strand, within fruB at 3257.650 kb on - strand, within fruB at 3257.665 kb on + strand, within fruB at 3257.726 kb on + strand, within fruB at 3257.726 kb on + strand, within fruB at 3257.730 kb on + strand, within fruB at 3257.788 kb on + strand, within fruB at 3257.788 kb on + strand, within fruB at 3257.789 kb on - strand, within fruB at 3257.792 kb on + strand, within fruB at 3257.792 kb on + strand, within fruB at 3257.792 kb on + strand, within fruB at 3257.792 kb on + strand, within fruB
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 3,255,070 - fruA LU632_RS17315 0.53 +0.2 3,255,070 - fruA LU632_RS17315 0.53 +0.3 3,255,140 + fruA LU632_RS17315 0.58 +0.4 3,255,140 + fruA LU632_RS17315 0.58 -1.3 3,255,141 - fruA LU632_RS17315 0.58 +0.1 3,255,213 + fruA LU632_RS17315 0.62 -0.5 3,255,297 + fruA LU632_RS17315 0.67 +0.6 3,255,308 + fruA LU632_RS17315 0.67 -0.5 3,255,308 + fruA LU632_RS17315 0.67 -0.0 3,255,319 - fruA LU632_RS17315 0.68 +0.8 3,255,348 + fruA LU632_RS17315 0.70 +0.3 3,255,348 + fruA LU632_RS17315 0.70 -0.3 3,255,349 - fruA LU632_RS17315 0.70 +0.7 3,255,364 - fruA LU632_RS17315 0.71 -0.3 3,255,496 - fruA LU632_RS17315 0.78 -0.0 3,255,496 - fruA LU632_RS17315 0.78 -0.1 3,255,496 - fruA LU632_RS17315 0.78 +1.0 3,255,652 + fruA LU632_RS17315 0.88 -2.1 3,255,653 - fruA LU632_RS17315 0.88 -1.4 3,255,653 - fruA LU632_RS17315 0.88 -0.3 3,255,706 - +0.5 3,255,711 + -1.3 3,255,711 + +1.3 3,255,711 + -0.4 3,255,712 - -0.3 3,255,799 - -0.3 3,255,844 + +0.3 3,255,845 - +0.3 3,255,862 + +0.3 3,255,862 + +1.3 3,255,863 - -0.3 3,255,863 - -0.3 3,255,882 + +0.3 3,255,892 + +0.0 3,255,892 + -0.7 3,255,892 + -1.3 3,256,028 + fruK LU632_RS17320 0.16 -2.3 3,256,201 + fruK LU632_RS17320 0.35 -0.3 3,256,201 + fruK LU632_RS17320 0.35 -0.3 3,256,202 - fruK LU632_RS17320 0.35 -0.3 3,256,323 + fruK LU632_RS17320 0.47 -3.1 3,256,324 - fruK LU632_RS17320 0.48 -3.3 3,256,548 + fruK LU632_RS17320 0.71 -0.3 3,256,549 - fruK LU632_RS17320 0.72 -0.3 3,257,016 - fruB LU632_RS17325 0.18 +0.5 3,257,179 + fruB LU632_RS17325 0.32 -1.3 3,257,179 + fruB LU632_RS17325 0.32 -0.7 3,257,179 + fruB LU632_RS17325 0.32 -0.3 3,257,180 - fruB LU632_RS17325 0.32 -0.3 3,257,180 - fruB LU632_RS17325 0.32 -1.9 3,257,182 - fruB LU632_RS17325 0.33 -0.3 3,257,198 - fruB LU632_RS17325 0.34 -0.3 3,257,198 - fruB LU632_RS17325 0.34 -0.3 3,257,477 + fruB LU632_RS17325 0.59 -2.9 3,257,606 + fruB LU632_RS17325 0.70 -1.3 3,257,607 - fruB LU632_RS17325 0.70 +0.7 3,257,650 - fruB LU632_RS17325 0.74 -1.2 3,257,665 + fruB LU632_RS17325 0.75 -0.6 3,257,726 + fruB LU632_RS17325 0.81 -1.9 3,257,726 + fruB LU632_RS17325 0.81 -0.3 3,257,730 + fruB LU632_RS17325 0.81 -0.6 3,257,788 + fruB LU632_RS17325 0.86 -0.3 3,257,788 + fruB LU632_RS17325 0.86 -1.2 3,257,789 - fruB LU632_RS17325 0.86 -0.7 3,257,792 + fruB LU632_RS17325 0.86 -0.7 3,257,792 + fruB LU632_RS17325 0.86 -0.5 3,257,792 + fruB LU632_RS17325 0.86 -0.9 3,257,792 + fruB LU632_RS17325 0.86 +0.1
Or see this region's nucleotide sequence