Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS16130 and pxpA are separated by 96 nucleotides pxpA and LU632_RS16140 overlap by 17 nucleotides LU632_RS16140 and pxpB overlap by 7 nucleotides pxpB and LU632_RS16150 are separated by 113 nucleotides
LU632_RS16130: LU632_RS16130 - helix-turn-helix domain-containing protein, at 3,050,219 to 3,050,575
_RS16130
LU632_RS16135: pxpA - 5-oxoprolinase subunit PxpA, at 3,050,672 to 3,051,410
pxpA
LU632_RS16140: LU632_RS16140 - biotin-dependent carboxyltransferase family protein, at 3,051,394 to 3,052,332
_RS16140
LU632_RS16145: pxpB - 5-oxoprolinase subunit PxpB, at 3,052,326 to 3,052,972
pxpB
LU632_RS16150: LU632_RS16150 - type 2 GTP cyclohydrolase I, at 3,053,086 to 3,053,829
_RS16150
Position (kb)
3051
3052
3053 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3050.607 kb on + strand at 3050.608 kb on - strand at 3050.608 kb on - strand at 3050.613 kb on - strand at 3050.613 kb on - strand at 3050.718 kb on - strand at 3050.779 kb on + strand, within pxpA at 3050.854 kb on - strand, within pxpA at 3051.082 kb on + strand, within pxpA at 3051.083 kb on - strand, within pxpA at 3051.427 kb on + strand at 3051.492 kb on + strand, within LU632_RS16140 at 3051.493 kb on - strand, within LU632_RS16140 at 3051.534 kb on - strand, within LU632_RS16140 at 3051.569 kb on + strand, within LU632_RS16140 at 3051.757 kb on + strand, within LU632_RS16140 at 3051.758 kb on - strand, within LU632_RS16140 at 3051.789 kb on - strand, within LU632_RS16140 at 3051.999 kb on - strand, within LU632_RS16140 at 3052.167 kb on + strand, within LU632_RS16140 at 3052.381 kb on + strand at 3052.411 kb on - strand, within pxpB at 3052.532 kb on - strand, within pxpB at 3052.535 kb on + strand, within pxpB at 3052.535 kb on + strand, within pxpB at 3052.536 kb on - strand, within pxpB at 3052.536 kb on - strand, within pxpB at 3052.536 kb on - strand, within pxpB at 3052.536 kb on - strand, within pxpB at 3052.546 kb on + strand, within pxpB at 3052.547 kb on - strand, within pxpB at 3052.547 kb on - strand, within pxpB at 3052.547 kb on - strand, within pxpB at 3052.693 kb on - strand, within pxpB at 3052.976 kb on - strand at 3053.004 kb on + strand at 3053.004 kb on + strand at 3053.018 kb on + strand at 3053.018 kb on + strand at 3053.018 kb on + strand at 3053.019 kb on - strand at 3053.019 kb on - strand at 3053.019 kb on - strand at 3053.019 kb on - strand at 3053.019 kb on - strand at 3053.019 kb on - strand at 3053.019 kb on - strand at 3053.023 kb on - strand at 3053.039 kb on - strand at 3053.130 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 3,050,607 + -0.0 3,050,608 - +1.3 3,050,608 - -1.8 3,050,613 - -0.0 3,050,613 - +0.7 3,050,718 - +0.3 3,050,779 + pxpA LU632_RS16135 0.14 +0.3 3,050,854 - pxpA LU632_RS16135 0.25 +1.5 3,051,082 + pxpA LU632_RS16135 0.55 +0.1 3,051,083 - pxpA LU632_RS16135 0.56 +0.1 3,051,427 + +0.3 3,051,492 + LU632_RS16140 0.10 +0.7 3,051,493 - LU632_RS16140 0.11 +0.7 3,051,534 - LU632_RS16140 0.15 -1.3 3,051,569 + LU632_RS16140 0.19 +0.5 3,051,757 + LU632_RS16140 0.39 -0.7 3,051,758 - LU632_RS16140 0.39 +0.6 3,051,789 - LU632_RS16140 0.42 +2.4 3,051,999 - LU632_RS16140 0.64 -0.3 3,052,167 + LU632_RS16140 0.82 +0.8 3,052,381 + +0.1 3,052,411 - pxpB LU632_RS16145 0.13 +0.3 3,052,532 - pxpB LU632_RS16145 0.32 -0.1 3,052,535 + pxpB LU632_RS16145 0.32 +1.1 3,052,535 + pxpB LU632_RS16145 0.32 -0.3 3,052,536 - pxpB LU632_RS16145 0.32 +0.2 3,052,536 - pxpB LU632_RS16145 0.32 +0.3 3,052,536 - pxpB LU632_RS16145 0.32 -0.4 3,052,536 - pxpB LU632_RS16145 0.32 +1.0 3,052,546 + pxpB LU632_RS16145 0.34 -0.3 3,052,547 - pxpB LU632_RS16145 0.34 -0.1 3,052,547 - pxpB LU632_RS16145 0.34 -0.1 3,052,547 - pxpB LU632_RS16145 0.34 -0.3 3,052,693 - pxpB LU632_RS16145 0.57 +0.5 3,052,976 - +0.1 3,053,004 + -0.3 3,053,004 + +0.2 3,053,018 + +0.7 3,053,018 + -1.2 3,053,018 + +0.7 3,053,019 - +0.1 3,053,019 - -1.5 3,053,019 - -0.3 3,053,019 - +0.3 3,053,019 - -0.5 3,053,019 - +0.3 3,053,019 - -0.3 3,053,023 - +0.5 3,053,039 - -0.3 3,053,130 - -0.6
Or see this region's nucleotide sequence