Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pncC and mltB are separated by 670 nucleotides mltB and LU632_RS15750 are separated by 209 nucleotides LU632_RS15750 and LU632_RS15755 are separated by 169 nucleotides
LU632_RS15740: pncC - nicotinamide-nucleotide amidase, at 2,979,057 to 2,979,551
pncC
LU632_RS15745: mltB - lytic murein transglycosylase B, at 2,980,222 to 2,981,301
mltB
LU632_RS15750: LU632_RS15750 - DJ-1/PfpI family protein, at 2,981,511 to 2,982,101
_RS15750
LU632_RS15755: LU632_RS15755 - IS256 family transposase, at 2,982,271 to 2,983,479
_RS15755
Position (kb)
2980
2981
2982 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2979.329 kb on - strand, within pncC at 2979.329 kb on - strand, within pncC at 2979.386 kb on - strand, within pncC at 2979.386 kb on - strand, within pncC at 2979.386 kb on - strand, within pncC at 2979.438 kb on - strand, within pncC at 2979.654 kb on + strand at 2979.655 kb on - strand at 2979.659 kb on - strand at 2979.662 kb on + strand at 2979.677 kb on - strand at 2979.682 kb on - strand at 2979.720 kb on - strand at 2979.724 kb on + strand at 2979.732 kb on + strand at 2979.733 kb on - strand at 2979.733 kb on - strand at 2979.804 kb on - strand at 2979.833 kb on - strand at 2979.845 kb on + strand at 2979.861 kb on - strand at 2979.867 kb on - strand at 2979.885 kb on + strand at 2979.886 kb on - strand at 2979.917 kb on + strand at 2979.918 kb on - strand at 2979.964 kb on + strand at 2979.983 kb on - strand at 2980.060 kb on - strand at 2980.103 kb on - strand at 2980.170 kb on - strand at 2980.170 kb on - strand at 2980.172 kb on + strand at 2980.193 kb on + strand at 2980.277 kb on + strand at 2980.293 kb on + strand at 2980.335 kb on - strand, within mltB at 2980.437 kb on + strand, within mltB at 2980.437 kb on + strand, within mltB at 2980.438 kb on - strand, within mltB at 2980.440 kb on - strand, within mltB at 2980.533 kb on - strand, within mltB at 2980.616 kb on + strand, within mltB at 2980.616 kb on + strand, within mltB at 2980.629 kb on + strand, within mltB at 2980.837 kb on - strand, within mltB at 2980.845 kb on + strand, within mltB at 2980.865 kb on + strand, within mltB at 2980.865 kb on + strand, within mltB at 2980.865 kb on + strand, within mltB at 2980.865 kb on + strand, within mltB at 2980.866 kb on - strand, within mltB at 2980.866 kb on - strand, within mltB at 2980.866 kb on - strand, within mltB at 2980.866 kb on - strand, within mltB at 2980.950 kb on + strand, within mltB at 2980.977 kb on + strand, within mltB at 2980.978 kb on - strand, within mltB at 2980.998 kb on - strand, within mltB at 2981.167 kb on - strand, within mltB at 2981.167 kb on - strand, within mltB at 2981.279 kb on - strand at 2981.295 kb on - strand at 2981.307 kb on - strand at 2981.311 kb on - strand at 2981.311 kb on - strand at 2981.327 kb on - strand at 2981.339 kb on - strand at 2981.372 kb on + strand at 2981.377 kb on - strand at 2981.377 kb on - strand at 2981.383 kb on - strand at 2981.482 kb on - strand at 2981.567 kb on + strand at 2981.662 kb on + strand, within LU632_RS15750 at 2981.662 kb on + strand, within LU632_RS15750 at 2981.663 kb on - strand, within LU632_RS15750 at 2981.680 kb on + strand, within LU632_RS15750 at 2981.701 kb on + strand, within LU632_RS15750 at 2981.701 kb on + strand, within LU632_RS15750 at 2981.701 kb on + strand, within LU632_RS15750 at 2981.702 kb on - strand, within LU632_RS15750 at 2981.850 kb on - strand, within LU632_RS15750 at 2981.877 kb on - strand, within LU632_RS15750 at 2981.895 kb on + strand, within LU632_RS15750 at 2981.895 kb on + strand, within LU632_RS15750 at 2981.896 kb on - strand, within LU632_RS15750 at 2981.896 kb on - strand, within LU632_RS15750 at 2981.896 kb on - strand, within LU632_RS15750 at 2981.972 kb on - strand, within LU632_RS15750 at 2982.057 kb on + strand at 2982.057 kb on + strand at 2982.087 kb on - strand at 2982.087 kb on - strand at 2982.088 kb on + strand at 2982.088 kb on + strand at 2982.089 kb on - strand at 2982.142 kb on + strand at 2982.154 kb on - strand at 2982.222 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 2,979,329 - pncC LU632_RS15740 0.55 +0.7 2,979,329 - pncC LU632_RS15740 0.55 +0.5 2,979,386 - pncC LU632_RS15740 0.66 +1.3 2,979,386 - pncC LU632_RS15740 0.66 +1.2 2,979,386 - pncC LU632_RS15740 0.66 -0.3 2,979,438 - pncC LU632_RS15740 0.77 -1.6 2,979,654 + -0.8 2,979,655 - -1.6 2,979,659 - +0.1 2,979,662 + +1.7 2,979,677 - -0.3 2,979,682 - -0.0 2,979,720 - -0.4 2,979,724 + +0.0 2,979,732 + +0.3 2,979,733 - +0.3 2,979,733 - -1.1 2,979,804 - -0.3 2,979,833 - +0.0 2,979,845 + +0.6 2,979,861 - +0.0 2,979,867 - -0.0 2,979,885 + +0.7 2,979,886 - -0.5 2,979,917 + -0.1 2,979,918 - -0.1 2,979,964 + +0.0 2,979,983 - +1.7 2,980,060 - +0.3 2,980,103 - -0.7 2,980,170 - +0.0 2,980,170 - -0.3 2,980,172 + -0.3 2,980,193 + +0.6 2,980,277 + +0.5 2,980,293 + +0.4 2,980,335 - mltB LU632_RS15745 0.10 -1.3 2,980,437 + mltB LU632_RS15745 0.20 -1.3 2,980,437 + mltB LU632_RS15745 0.20 +1.0 2,980,438 - mltB LU632_RS15745 0.20 -0.4 2,980,440 - mltB LU632_RS15745 0.20 -0.6 2,980,533 - mltB LU632_RS15745 0.29 +0.7 2,980,616 + mltB LU632_RS15745 0.36 +1.0 2,980,616 + mltB LU632_RS15745 0.36 -0.6 2,980,629 + mltB LU632_RS15745 0.38 -0.4 2,980,837 - mltB LU632_RS15745 0.57 -0.5 2,980,845 + mltB LU632_RS15745 0.58 -0.9 2,980,865 + mltB LU632_RS15745 0.60 +0.5 2,980,865 + mltB LU632_RS15745 0.60 +0.3 2,980,865 + mltB LU632_RS15745 0.60 +1.3 2,980,865 + mltB LU632_RS15745 0.60 -0.3 2,980,866 - mltB LU632_RS15745 0.60 +1.3 2,980,866 - mltB LU632_RS15745 0.60 -0.3 2,980,866 - mltB LU632_RS15745 0.60 +0.3 2,980,866 - mltB LU632_RS15745 0.60 +0.4 2,980,950 + mltB LU632_RS15745 0.67 +0.6 2,980,977 + mltB LU632_RS15745 0.70 +1.1 2,980,978 - mltB LU632_RS15745 0.70 -0.3 2,980,998 - mltB LU632_RS15745 0.72 -0.9 2,981,167 - mltB LU632_RS15745 0.88 -0.3 2,981,167 - mltB LU632_RS15745 0.88 -0.9 2,981,279 - -0.3 2,981,295 - -1.0 2,981,307 - -0.5 2,981,311 - +0.4 2,981,311 - -0.5 2,981,327 - +0.3 2,981,339 - -0.3 2,981,372 + +0.7 2,981,377 - -0.1 2,981,377 - -0.7 2,981,383 - -0.3 2,981,482 - +0.2 2,981,567 + +1.3 2,981,662 + LU632_RS15750 0.26 -1.9 2,981,662 + LU632_RS15750 0.26 +0.7 2,981,663 - LU632_RS15750 0.26 -0.1 2,981,680 + LU632_RS15750 0.29 +0.6 2,981,701 + LU632_RS15750 0.32 -0.1 2,981,701 + LU632_RS15750 0.32 +0.4 2,981,701 + LU632_RS15750 0.32 +1.7 2,981,702 - LU632_RS15750 0.32 +0.1 2,981,850 - LU632_RS15750 0.57 -0.6 2,981,877 - LU632_RS15750 0.62 -0.3 2,981,895 + LU632_RS15750 0.65 +1.3 2,981,895 + LU632_RS15750 0.65 +0.7 2,981,896 - LU632_RS15750 0.65 +0.0 2,981,896 - LU632_RS15750 0.65 -0.6 2,981,896 - LU632_RS15750 0.65 +2.3 2,981,972 - LU632_RS15750 0.78 -0.2 2,982,057 + -0.2 2,982,057 + +0.1 2,982,087 - -0.3 2,982,087 - +0.5 2,982,088 + +0.7 2,982,088 + +0.1 2,982,089 - +0.7 2,982,142 + -0.4 2,982,154 - -2.6 2,982,222 - -1.9
Or see this region's nucleotide sequence