Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ffh and LU632_RS15675 are separated by 159 nucleotides LU632_RS15675 and LU632_RS15680 are separated by 25 nucleotides LU632_RS15680 and luxS are separated by 58 nucleotides luxS and gshA are separated by 150 nucleotides
LU632_RS15670: ffh - signal recognition particle protein, at 2,966,166 to 2,967,527
ffh
LU632_RS15675: LU632_RS15675 - inner membrane protein YpjD, at 2,967,687 to 2,968,478
_RS15675
LU632_RS15680: LU632_RS15680 - HlyC/CorC family transporter, at 2,968,504 to 2,969,790
_RS15680
LU632_RS15685: luxS - S-ribosylhomocysteine lyase, at 2,969,849 to 2,970,364
luxS
LU632_RS15690: gshA - glutamate--cysteine ligase, at 2,970,515 to 2,972,080
gshA
Position (kb)
2968
2969
2970 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2967.544 kb on - strand at 2967.580 kb on - strand at 2967.582 kb on - strand at 2967.649 kb on - strand at 2967.650 kb on + strand at 2967.650 kb on + strand at 2967.687 kb on - strand at 2967.687 kb on - strand at 2967.687 kb on - strand at 2967.694 kb on + strand at 2967.695 kb on - strand at 2967.695 kb on - strand at 2967.770 kb on + strand, within LU632_RS15675 at 2967.770 kb on + strand, within LU632_RS15675 at 2967.771 kb on - strand, within LU632_RS15675 at 2967.771 kb on - strand, within LU632_RS15675 at 2967.771 kb on - strand, within LU632_RS15675 at 2967.790 kb on + strand, within LU632_RS15675 at 2967.813 kb on - strand, within LU632_RS15675 at 2967.813 kb on - strand, within LU632_RS15675 at 2967.824 kb on + strand, within LU632_RS15675 at 2967.832 kb on + strand, within LU632_RS15675 at 2967.832 kb on + strand, within LU632_RS15675 at 2967.832 kb on + strand, within LU632_RS15675 at 2967.832 kb on + strand, within LU632_RS15675 at 2967.835 kb on - strand, within LU632_RS15675 at 2967.835 kb on - strand, within LU632_RS15675 at 2967.835 kb on - strand, within LU632_RS15675 at 2967.881 kb on - strand, within LU632_RS15675 at 2967.884 kb on + strand, within LU632_RS15675 at 2967.884 kb on + strand, within LU632_RS15675 at 2967.920 kb on + strand, within LU632_RS15675 at 2967.920 kb on + strand, within LU632_RS15675 at 2967.921 kb on - strand, within LU632_RS15675 at 2967.989 kb on + strand, within LU632_RS15675 at 2968.043 kb on + strand, within LU632_RS15675 at 2968.043 kb on + strand, within LU632_RS15675 at 2968.044 kb on - strand, within LU632_RS15675 at 2968.044 kb on - strand, within LU632_RS15675 at 2968.096 kb on + strand, within LU632_RS15675 at 2968.096 kb on + strand, within LU632_RS15675 at 2968.096 kb on + strand, within LU632_RS15675 at 2968.097 kb on - strand, within LU632_RS15675 at 2968.114 kb on - strand, within LU632_RS15675 at 2968.126 kb on + strand, within LU632_RS15675 at 2968.126 kb on + strand, within LU632_RS15675 at 2968.126 kb on + strand, within LU632_RS15675 at 2968.126 kb on + strand, within LU632_RS15675 at 2968.126 kb on + strand, within LU632_RS15675 at 2968.127 kb on - strand, within LU632_RS15675 at 2968.127 kb on - strand, within LU632_RS15675 at 2968.127 kb on - strand, within LU632_RS15675 at 2968.217 kb on + strand, within LU632_RS15675 at 2968.217 kb on + strand, within LU632_RS15675 at 2968.218 kb on - strand, within LU632_RS15675 at 2968.235 kb on - strand, within LU632_RS15675 at 2968.272 kb on + strand, within LU632_RS15675 at 2968.272 kb on + strand, within LU632_RS15675 at 2968.272 kb on + strand, within LU632_RS15675 at 2968.273 kb on - strand, within LU632_RS15675 at 2968.273 kb on - strand, within LU632_RS15675 at 2968.273 kb on - strand, within LU632_RS15675 at 2968.273 kb on - strand, within LU632_RS15675 at 2968.275 kb on - strand, within LU632_RS15675 at 2968.337 kb on + strand, within LU632_RS15675 at 2968.337 kb on + strand, within LU632_RS15675 at 2968.337 kb on + strand, within LU632_RS15675 at 2968.337 kb on + strand, within LU632_RS15675 at 2968.338 kb on - strand, within LU632_RS15675 at 2968.343 kb on + strand, within LU632_RS15675 at 2968.427 kb on + strand at 2968.427 kb on + strand at 2968.438 kb on - strand at 2968.438 kb on - strand at 2968.533 kb on + strand at 2968.534 kb on - strand at 2968.537 kb on - strand at 2968.670 kb on + strand, within LU632_RS15680 at 2968.671 kb on - strand, within LU632_RS15680 at 2968.671 kb on - strand, within LU632_RS15680 at 2968.713 kb on + strand, within LU632_RS15680 at 2968.713 kb on + strand, within LU632_RS15680 at 2968.713 kb on + strand, within LU632_RS15680 at 2968.725 kb on + strand, within LU632_RS15680 at 2968.789 kb on - strand, within LU632_RS15680 at 2968.838 kb on + strand, within LU632_RS15680 at 2968.891 kb on - strand, within LU632_RS15680 at 2968.918 kb on - strand, within LU632_RS15680 at 2968.940 kb on + strand, within LU632_RS15680 at 2968.941 kb on - strand, within LU632_RS15680 at 2968.947 kb on + strand, within LU632_RS15680 at 2968.947 kb on + strand, within LU632_RS15680 at 2968.948 kb on - strand, within LU632_RS15680 at 2968.948 kb on - strand, within LU632_RS15680 at 2968.948 kb on - strand, within LU632_RS15680 at 2968.948 kb on - strand, within LU632_RS15680 at 2968.999 kb on - strand, within LU632_RS15680 at 2969.039 kb on - strand, within LU632_RS15680 at 2969.094 kb on - strand, within LU632_RS15680 at 2969.110 kb on - strand, within LU632_RS15680 at 2969.124 kb on + strand, within LU632_RS15680 at 2969.214 kb on + strand, within LU632_RS15680 at 2969.214 kb on + strand, within LU632_RS15680 at 2969.214 kb on + strand, within LU632_RS15680 at 2969.217 kb on - strand, within LU632_RS15680 at 2969.244 kb on + strand, within LU632_RS15680 at 2969.245 kb on - strand, within LU632_RS15680 at 2969.247 kb on + strand, within LU632_RS15680 at 2969.247 kb on + strand, within LU632_RS15680 at 2969.248 kb on - strand, within LU632_RS15680 at 2969.248 kb on - strand, within LU632_RS15680 at 2969.250 kb on + strand, within LU632_RS15680 at 2969.250 kb on + strand, within LU632_RS15680 at 2969.316 kb on + strand, within LU632_RS15680 at 2969.344 kb on + strand, within LU632_RS15680 at 2969.366 kb on + strand, within LU632_RS15680 at 2969.381 kb on + strand, within LU632_RS15680 at 2969.395 kb on - strand, within LU632_RS15680 at 2969.428 kb on + strand, within LU632_RS15680 at 2969.429 kb on - strand, within LU632_RS15680 at 2969.568 kb on + strand, within LU632_RS15680 at 2969.568 kb on + strand, within LU632_RS15680 at 2969.582 kb on + strand, within LU632_RS15680 at 2969.586 kb on + strand, within LU632_RS15680 at 2969.587 kb on - strand, within LU632_RS15680 at 2969.661 kb on + strand, within LU632_RS15680 at 2969.688 kb on + strand at 2969.699 kb on - strand at 2969.705 kb on + strand at 2969.706 kb on - strand at 2969.706 kb on - strand at 2969.706 kb on - strand at 2969.709 kb on + strand at 2969.709 kb on + strand at 2969.710 kb on - strand at 2969.730 kb on + strand at 2969.731 kb on + strand at 2969.850 kb on + strand at 2969.851 kb on - strand at 2969.858 kb on + strand at 2969.940 kb on - strand, within luxS at 2970.102 kb on + strand, within luxS at 2970.103 kb on - strand, within luxS at 2970.134 kb on - strand, within luxS at 2970.134 kb on - strand, within luxS at 2970.176 kb on - strand, within luxS at 2970.268 kb on + strand, within luxS at 2970.364 kb on + strand at 2970.365 kb on - strand at 2970.365 kb on - strand at 2970.370 kb on + strand at 2970.432 kb on + strand at 2970.666 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 2,967,544 - -0.1 2,967,580 - -1.3 2,967,582 - +0.1 2,967,649 - -1.6 2,967,650 + +0.3 2,967,650 + +0.3 2,967,687 - +1.0 2,967,687 - +0.4 2,967,687 - -0.4 2,967,694 + -0.1 2,967,695 - +0.5 2,967,695 - -1.3 2,967,770 + LU632_RS15675 0.10 +0.5 2,967,770 + LU632_RS15675 0.10 +2.0 2,967,771 - LU632_RS15675 0.11 -0.1 2,967,771 - LU632_RS15675 0.11 +0.9 2,967,771 - LU632_RS15675 0.11 +0.7 2,967,790 + LU632_RS15675 0.13 +0.2 2,967,813 - LU632_RS15675 0.16 +0.3 2,967,813 - LU632_RS15675 0.16 +0.6 2,967,824 + LU632_RS15675 0.17 +0.4 2,967,832 + LU632_RS15675 0.18 +0.4 2,967,832 + LU632_RS15675 0.18 +0.7 2,967,832 + LU632_RS15675 0.18 +0.4 2,967,832 + LU632_RS15675 0.18 -0.0 2,967,835 - LU632_RS15675 0.19 +2.0 2,967,835 - LU632_RS15675 0.19 +0.9 2,967,835 - LU632_RS15675 0.19 +1.3 2,967,881 - LU632_RS15675 0.24 +1.3 2,967,884 + LU632_RS15675 0.25 +0.3 2,967,884 + LU632_RS15675 0.25 +0.4 2,967,920 + LU632_RS15675 0.29 +0.1 2,967,920 + LU632_RS15675 0.29 +0.6 2,967,921 - LU632_RS15675 0.30 -1.9 2,967,989 + LU632_RS15675 0.38 -0.2 2,968,043 + LU632_RS15675 0.45 -0.9 2,968,043 + LU632_RS15675 0.45 -0.4 2,968,044 - LU632_RS15675 0.45 +0.4 2,968,044 - LU632_RS15675 0.45 +1.1 2,968,096 + LU632_RS15675 0.52 -0.9 2,968,096 + LU632_RS15675 0.52 -0.3 2,968,096 + LU632_RS15675 0.52 -0.9 2,968,097 - LU632_RS15675 0.52 +1.5 2,968,114 - LU632_RS15675 0.54 +0.7 2,968,126 + LU632_RS15675 0.55 -1.3 2,968,126 + LU632_RS15675 0.55 +0.0 2,968,126 + LU632_RS15675 0.55 -0.7 2,968,126 + LU632_RS15675 0.55 +0.2 2,968,126 + LU632_RS15675 0.55 -0.8 2,968,127 - LU632_RS15675 0.56 +0.7 2,968,127 - LU632_RS15675 0.56 +0.9 2,968,127 - LU632_RS15675 0.56 +1.3 2,968,217 + LU632_RS15675 0.67 -0.8 2,968,217 + LU632_RS15675 0.67 -0.3 2,968,218 - LU632_RS15675 0.67 +1.0 2,968,235 - LU632_RS15675 0.69 +0.4 2,968,272 + LU632_RS15675 0.74 -3.3 2,968,272 + LU632_RS15675 0.74 -0.5 2,968,272 + LU632_RS15675 0.74 -1.1 2,968,273 - LU632_RS15675 0.74 +1.1 2,968,273 - LU632_RS15675 0.74 +0.7 2,968,273 - LU632_RS15675 0.74 +0.7 2,968,273 - LU632_RS15675 0.74 +1.1 2,968,275 - LU632_RS15675 0.74 +1.1 2,968,337 + LU632_RS15675 0.82 -0.4 2,968,337 + LU632_RS15675 0.82 -0.3 2,968,337 + LU632_RS15675 0.82 -0.2 2,968,337 + LU632_RS15675 0.82 -1.3 2,968,338 - LU632_RS15675 0.82 +1.1 2,968,343 + LU632_RS15675 0.83 -0.3 2,968,427 + -0.8 2,968,427 + -0.3 2,968,438 - +0.6 2,968,438 - +1.7 2,968,533 + +0.3 2,968,534 - +0.7 2,968,537 - +1.0 2,968,670 + LU632_RS15680 0.13 +0.0 2,968,671 - LU632_RS15680 0.13 +1.3 2,968,671 - LU632_RS15680 0.13 +1.7 2,968,713 + LU632_RS15680 0.16 -1.0 2,968,713 + LU632_RS15680 0.16 +1.4 2,968,713 + LU632_RS15680 0.16 +0.9 2,968,725 + LU632_RS15680 0.17 +1.5 2,968,789 - LU632_RS15680 0.22 +1.1 2,968,838 + LU632_RS15680 0.26 +0.3 2,968,891 - LU632_RS15680 0.30 +1.0 2,968,918 - LU632_RS15680 0.32 +1.3 2,968,940 + LU632_RS15680 0.34 +1.5 2,968,941 - LU632_RS15680 0.34 +2.1 2,968,947 + LU632_RS15680 0.34 +2.3 2,968,947 + LU632_RS15680 0.34 +1.8 2,968,948 - LU632_RS15680 0.34 +0.9 2,968,948 - LU632_RS15680 0.34 +1.0 2,968,948 - LU632_RS15680 0.34 +1.8 2,968,948 - LU632_RS15680 0.34 +0.5 2,968,999 - LU632_RS15680 0.38 +2.2 2,969,039 - LU632_RS15680 0.42 -0.0 2,969,094 - LU632_RS15680 0.46 +1.4 2,969,110 - LU632_RS15680 0.47 +1.1 2,969,124 + LU632_RS15680 0.48 +1.3 2,969,214 + LU632_RS15680 0.55 +2.0 2,969,214 + LU632_RS15680 0.55 +1.7 2,969,214 + LU632_RS15680 0.55 +2.1 2,969,217 - LU632_RS15680 0.55 -0.0 2,969,244 + LU632_RS15680 0.57 +2.0 2,969,245 - LU632_RS15680 0.58 +0.1 2,969,247 + LU632_RS15680 0.58 +0.4 2,969,247 + LU632_RS15680 0.58 +2.1 2,969,248 - LU632_RS15680 0.58 +1.1 2,969,248 - LU632_RS15680 0.58 +0.7 2,969,250 + LU632_RS15680 0.58 +1.5 2,969,250 + LU632_RS15680 0.58 +1.8 2,969,316 + LU632_RS15680 0.63 +1.8 2,969,344 + LU632_RS15680 0.65 +1.2 2,969,366 + LU632_RS15680 0.67 +1.2 2,969,381 + LU632_RS15680 0.68 +0.1 2,969,395 - LU632_RS15680 0.69 +1.8 2,969,428 + LU632_RS15680 0.72 +1.2 2,969,429 - LU632_RS15680 0.72 +2.2 2,969,568 + LU632_RS15680 0.83 +1.5 2,969,568 + LU632_RS15680 0.83 +1.3 2,969,582 + LU632_RS15680 0.84 +0.8 2,969,586 + LU632_RS15680 0.84 +0.7 2,969,587 - LU632_RS15680 0.84 +1.6 2,969,661 + LU632_RS15680 0.90 +1.8 2,969,688 + +0.9 2,969,699 - +1.2 2,969,705 + +1.2 2,969,706 - +2.0 2,969,706 - +1.0 2,969,706 - +0.7 2,969,709 + +2.1 2,969,709 + +1.7 2,969,710 - +0.8 2,969,730 + +1.4 2,969,731 + +0.3 2,969,850 + +0.1 2,969,851 - +1.1 2,969,858 + +0.3 2,969,940 - luxS LU632_RS15685 0.18 -0.3 2,970,102 + luxS LU632_RS15685 0.49 +0.7 2,970,103 - luxS LU632_RS15685 0.49 +1.3 2,970,134 - luxS LU632_RS15685 0.55 -1.2 2,970,134 - luxS LU632_RS15685 0.55 +0.3 2,970,176 - luxS LU632_RS15685 0.63 +0.1 2,970,268 + luxS LU632_RS15685 0.81 +1.0 2,970,364 + +0.2 2,970,365 - -1.3 2,970,365 - -0.5 2,970,370 + +2.0 2,970,432 + -1.3 2,970,666 + -1.3
Or see this region's nucleotide sequence