Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS14985 and LU632_RS14990 are separated by 171 nucleotides LU632_RS14990 and LU632_RS14995 overlap by 4 nucleotides LU632_RS14995 and LU632_RS15000 are separated by 227 nucleotides LU632_RS15000 and LU632_RS15005 are separated by 432 nucleotides
LU632_RS14985: LU632_RS14985 - IS91 family transposase, at 2,848,361 to 2,849,560
_RS14985
LU632_RS14990: LU632_RS14990 - phage terminase large subunit, at 2,849,732 to 2,850,526
_RS14990
LU632_RS14995: LU632_RS14995 - ubiquitin carboxyl-hydrolase, at 2,850,523 to 2,850,957
_RS14995
LU632_RS15000: LU632_RS15000 - hypothetical protein, at 2,851,185 to 2,851,376
_RS15000
LU632_RS15005: LU632_RS15005 - hypothetical protein, at 2,851,809 to 2,852,090
_RS15005
Position (kb)
2850
2851 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2849.726 kb on + strand at 2849.831 kb on - strand, within LU632_RS14990 at 2849.866 kb on - strand, within LU632_RS14990 at 2849.889 kb on + strand, within LU632_RS14990 at 2849.889 kb on + strand, within LU632_RS14990 at 2849.889 kb on + strand, within LU632_RS14990 at 2849.890 kb on - strand, within LU632_RS14990 at 2849.967 kb on + strand, within LU632_RS14990 at 2849.967 kb on + strand, within LU632_RS14990 at 2849.967 kb on + strand, within LU632_RS14990 at 2849.968 kb on - strand, within LU632_RS14990 at 2849.968 kb on - strand, within LU632_RS14990 at 2849.968 kb on - strand, within LU632_RS14990 at 2850.207 kb on + strand, within LU632_RS14990 at 2850.207 kb on + strand, within LU632_RS14990 at 2850.237 kb on + strand, within LU632_RS14990 at 2850.308 kb on - strand, within LU632_RS14990 at 2850.544 kb on + strand at 2850.660 kb on + strand, within LU632_RS14995 at 2850.688 kb on - strand, within LU632_RS14995 at 2850.691 kb on + strand, within LU632_RS14995 at 2850.692 kb on - strand, within LU632_RS14995 at 2850.804 kb on + strand, within LU632_RS14995 at 2850.825 kb on + strand, within LU632_RS14995 at 2850.895 kb on + strand, within LU632_RS14995 at 2850.895 kb on + strand, within LU632_RS14995 at 2850.895 kb on + strand, within LU632_RS14995 at 2850.895 kb on + strand, within LU632_RS14995 at 2850.895 kb on + strand, within LU632_RS14995 at 2850.895 kb on + strand, within LU632_RS14995 at 2850.895 kb on + strand, within LU632_RS14995 at 2850.895 kb on + strand, within LU632_RS14995 at 2850.895 kb on + strand, within LU632_RS14995 at 2850.895 kb on + strand, within LU632_RS14995 at 2850.895 kb on + strand, within LU632_RS14995 at 2850.895 kb on + strand, within LU632_RS14995 at 2850.895 kb on + strand, within LU632_RS14995 at 2850.896 kb on - strand, within LU632_RS14995 at 2850.896 kb on - strand, within LU632_RS14995 at 2850.896 kb on - strand, within LU632_RS14995 at 2850.896 kb on - strand, within LU632_RS14995 at 2850.896 kb on - strand, within LU632_RS14995 at 2850.896 kb on - strand, within LU632_RS14995 at 2850.916 kb on + strand at 2850.991 kb on + strand at 2850.992 kb on - strand at 2851.062 kb on - strand at 2851.097 kb on - strand at 2851.103 kb on - strand at 2851.103 kb on - strand at 2851.179 kb on + strand at 2851.179 kb on + strand at 2851.180 kb on - strand at 2851.299 kb on + strand, within LU632_RS15000 at 2851.325 kb on + strand, within LU632_RS15000 at 2851.326 kb on - strand, within LU632_RS15000 at 2851.364 kb on + strand at 2851.397 kb on + strand at 2851.556 kb on - strand at 2851.557 kb on + strand at 2851.557 kb on + strand at 2851.557 kb on + strand at 2851.557 kb on + strand at 2851.558 kb on - strand at 2851.558 kb on - strand at 2851.694 kb on - strand at 2851.709 kb on + strand at 2851.756 kb on - strand at 2851.761 kb on + strand at 2851.761 kb on + strand at 2851.761 kb on + strand at 2851.761 kb on + strand at 2851.797 kb on - strand at 2851.799 kb on + strand at 2851.799 kb on + strand at 2851.799 kb on + strand at 2851.800 kb on - strand at 2851.800 kb on - strand at 2851.810 kb on + strand at 2851.811 kb on - strand at 2851.950 kb on - strand, within LU632_RS15005 at 2851.950 kb on - strand, within LU632_RS15005
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 2,849,726 + +1.2 2,849,831 - LU632_RS14990 0.12 +0.5 2,849,866 - LU632_RS14990 0.17 +0.8 2,849,889 + LU632_RS14990 0.20 -0.3 2,849,889 + LU632_RS14990 0.20 -1.3 2,849,889 + LU632_RS14990 0.20 -2.0 2,849,890 - LU632_RS14990 0.20 -0.3 2,849,967 + LU632_RS14990 0.30 +1.7 2,849,967 + LU632_RS14990 0.30 +0.4 2,849,967 + LU632_RS14990 0.30 +0.3 2,849,968 - LU632_RS14990 0.30 -1.3 2,849,968 - LU632_RS14990 0.30 +0.2 2,849,968 - LU632_RS14990 0.30 +0.3 2,850,207 + LU632_RS14990 0.60 +1.7 2,850,207 + LU632_RS14990 0.60 -0.6 2,850,237 + LU632_RS14990 0.64 -0.2 2,850,308 - LU632_RS14990 0.72 +0.0 2,850,544 + +0.2 2,850,660 + LU632_RS14995 0.31 +0.4 2,850,688 - LU632_RS14995 0.38 -0.3 2,850,691 + LU632_RS14995 0.39 -0.6 2,850,692 - LU632_RS14995 0.39 -1.9 2,850,804 + LU632_RS14995 0.65 +0.7 2,850,825 + LU632_RS14995 0.69 -0.9 2,850,895 + LU632_RS14995 0.86 +0.4 2,850,895 + LU632_RS14995 0.86 -0.5 2,850,895 + LU632_RS14995 0.86 +2.0 2,850,895 + LU632_RS14995 0.86 -0.3 2,850,895 + LU632_RS14995 0.86 +0.6 2,850,895 + LU632_RS14995 0.86 +0.3 2,850,895 + LU632_RS14995 0.86 +0.3 2,850,895 + LU632_RS14995 0.86 -3.1 2,850,895 + LU632_RS14995 0.86 -0.3 2,850,895 + LU632_RS14995 0.86 -2.6 2,850,895 + LU632_RS14995 0.86 -0.2 2,850,895 + LU632_RS14995 0.86 -1.7 2,850,895 + LU632_RS14995 0.86 -1.9 2,850,896 - LU632_RS14995 0.86 -0.3 2,850,896 - LU632_RS14995 0.86 +0.1 2,850,896 - LU632_RS14995 0.86 +0.3 2,850,896 - LU632_RS14995 0.86 -0.3 2,850,896 - LU632_RS14995 0.86 -0.7 2,850,896 - LU632_RS14995 0.86 -2.3 2,850,916 + +0.1 2,850,991 + -1.0 2,850,992 - +1.1 2,851,062 - -1.0 2,851,097 - +0.3 2,851,103 - +0.3 2,851,103 - -0.1 2,851,179 + -1.3 2,851,179 + +0.1 2,851,180 - -2.6 2,851,299 + LU632_RS15000 0.59 +0.8 2,851,325 + LU632_RS15000 0.73 -0.0 2,851,326 - LU632_RS15000 0.73 +0.4 2,851,364 + +0.4 2,851,397 + +1.7 2,851,556 - -0.1 2,851,557 + -0.1 2,851,557 + +0.4 2,851,557 + +0.4 2,851,557 + +0.5 2,851,558 - -0.7 2,851,558 - +0.1 2,851,694 - -0.3 2,851,709 + -0.3 2,851,756 - -0.3 2,851,761 + -0.7 2,851,761 + -0.7 2,851,761 + -0.1 2,851,761 + +0.5 2,851,797 - +0.6 2,851,799 + +0.4 2,851,799 + -0.3 2,851,799 + +1.1 2,851,800 - +1.2 2,851,800 - +1.7 2,851,810 + +0.7 2,851,811 - -1.3 2,851,950 - LU632_RS15005 0.50 -0.3 2,851,950 - LU632_RS15005 0.50 -0.3
Or see this region's nucleotide sequence