Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS12845 and cls are separated by 557 nucleotides cls and LU632_RS12855 are separated by 45 nucleotides LU632_RS12855 and oppF are separated by 227 nucleotides
LU632_RS12845: LU632_RS12845 - IS256 family transposase, at 2,444,049 to 2,445,244
_RS12845
LU632_RS12850: cls - cardiolipin synthase, at 2,445,802 to 2,447,262
cls
LU632_RS12855: LU632_RS12855 - HI1450 family dsDNA-mimic protein, at 2,447,308 to 2,447,631
_RS12855
LU632_RS12860: oppF - murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF, at 2,447,859 to 2,448,860
oppF
Position (kb)
2445
2446
2447
2448 Strain fitness (log2 ratio)
-2
-1
0
1 at 2445.267 kb on + strand at 2445.271 kb on + strand at 2445.303 kb on - strand at 2445.493 kb on + strand at 2445.542 kb on - strand at 2445.586 kb on - strand at 2445.626 kb on + strand at 2445.626 kb on + strand at 2445.626 kb on + strand at 2445.627 kb on - strand at 2445.658 kb on + strand at 2445.936 kb on + strand at 2446.118 kb on + strand, within cls at 2446.202 kb on + strand, within cls at 2446.498 kb on + strand, within cls at 2446.502 kb on + strand, within cls at 2446.508 kb on - strand, within cls at 2446.627 kb on + strand, within cls at 2446.689 kb on + strand, within cls at 2446.719 kb on + strand, within cls at 2447.017 kb on - strand, within cls at 2447.362 kb on - strand, within LU632_RS12855 at 2447.407 kb on - strand, within LU632_RS12855 at 2447.520 kb on - strand, within LU632_RS12855 at 2447.601 kb on + strand at 2447.602 kb on - strand at 2447.638 kb on - strand at 2447.765 kb on + strand at 2447.768 kb on + strand at 2447.768 kb on + strand at 2447.768 kb on + strand at 2447.768 kb on + strand at 2447.771 kb on + strand at 2447.771 kb on + strand at 2447.771 kb on + strand at 2448.021 kb on + strand, within oppF at 2448.021 kb on + strand, within oppF at 2448.022 kb on - strand, within oppF at 2448.083 kb on + strand, within oppF at 2448.086 kb on - strand, within oppF at 2448.107 kb on - strand, within oppF at 2448.118 kb on + strand, within oppF at 2448.118 kb on + strand, within oppF at 2448.119 kb on - strand, within oppF at 2448.148 kb on + strand, within oppF at 2448.149 kb on - strand, within oppF at 2448.149 kb on - strand, within oppF at 2448.203 kb on + strand, within oppF at 2448.203 kb on + strand, within oppF at 2448.204 kb on - strand, within oppF at 2448.207 kb on + strand, within oppF
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 2,445,267 + +0.3 2,445,271 + +0.9 2,445,303 - -0.9 2,445,493 + -0.9 2,445,542 - +0.6 2,445,586 - +0.0 2,445,626 + +1.0 2,445,626 + +1.3 2,445,626 + -0.7 2,445,627 - -0.2 2,445,658 + +0.7 2,445,936 + -1.3 2,446,118 + cls LU632_RS12850 0.22 -1.3 2,446,202 + cls LU632_RS12850 0.27 -0.3 2,446,498 + cls LU632_RS12850 0.48 -1.3 2,446,502 + cls LU632_RS12850 0.48 -2.3 2,446,508 - cls LU632_RS12850 0.48 -1.3 2,446,627 + cls LU632_RS12850 0.56 -0.3 2,446,689 + cls LU632_RS12850 0.61 -1.3 2,446,719 + cls LU632_RS12850 0.63 -0.3 2,447,017 - cls LU632_RS12850 0.83 -0.3 2,447,362 - LU632_RS12855 0.17 -0.6 2,447,407 - LU632_RS12855 0.31 +0.2 2,447,520 - LU632_RS12855 0.65 +0.5 2,447,601 + +1.0 2,447,602 - +1.3 2,447,638 - +0.4 2,447,765 + +0.7 2,447,768 + -0.2 2,447,768 + +0.0 2,447,768 + +0.5 2,447,768 + +0.7 2,447,771 + +0.7 2,447,771 + -2.3 2,447,771 + -0.3 2,448,021 + oppF LU632_RS12860 0.16 +1.7 2,448,021 + oppF LU632_RS12860 0.16 -1.3 2,448,022 - oppF LU632_RS12860 0.16 -0.3 2,448,083 + oppF LU632_RS12860 0.22 -0.3 2,448,086 - oppF LU632_RS12860 0.23 -0.3 2,448,107 - oppF LU632_RS12860 0.25 -1.3 2,448,118 + oppF LU632_RS12860 0.26 -1.3 2,448,118 + oppF LU632_RS12860 0.26 -1.0 2,448,119 - oppF LU632_RS12860 0.26 -1.6 2,448,148 + oppF LU632_RS12860 0.29 -0.7 2,448,149 - oppF LU632_RS12860 0.29 +0.1 2,448,149 - oppF LU632_RS12860 0.29 +0.3 2,448,203 + oppF LU632_RS12860 0.34 -0.5 2,448,203 + oppF LU632_RS12860 0.34 +0.9 2,448,204 - oppF LU632_RS12860 0.34 -1.3 2,448,207 + oppF LU632_RS12860 0.35 -0.6
Or see this region's nucleotide sequence