Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpCF and trpB are separated by 50 nucleotides trpB and trpA overlap by 1 nucleotides trpA and LU632_RS12780 are separated by 157 nucleotides
LU632_RS12765: trpCF - bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF, at 2,429,524 to 2,430,885
trpCF
LU632_RS12770: trpB - tryptophan synthase subunit beta, at 2,430,936 to 2,432,126
trpB
LU632_RS12775: trpA - tryptophan synthase subunit alpha, at 2,432,126 to 2,432,932
trpA
LU632_RS12780: LU632_RS12780 - BON domain-containing protein, at 2,433,090 to 2,433,404
_RS12780
Position (kb)
2430
2431
2432
2433 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2429.962 kb on + strand, within trpCF at 2429.988 kb on + strand, within trpCF at 2429.989 kb on - strand, within trpCF at 2429.989 kb on - strand, within trpCF at 2430.030 kb on - strand, within trpCF at 2430.150 kb on + strand, within trpCF at 2430.151 kb on - strand, within trpCF at 2430.178 kb on - strand, within trpCF at 2430.184 kb on + strand, within trpCF at 2430.184 kb on + strand, within trpCF at 2430.298 kb on - strand, within trpCF at 2430.358 kb on + strand, within trpCF at 2430.359 kb on - strand, within trpCF at 2430.441 kb on + strand, within trpCF at 2430.441 kb on + strand, within trpCF at 2430.442 kb on - strand, within trpCF at 2430.442 kb on - strand, within trpCF at 2430.445 kb on + strand, within trpCF at 2430.445 kb on + strand, within trpCF at 2430.446 kb on - strand, within trpCF at 2430.584 kb on + strand, within trpCF at 2430.590 kb on + strand, within trpCF at 2430.609 kb on + strand, within trpCF at 2430.610 kb on - strand, within trpCF at 2430.843 kb on + strand at 2430.844 kb on - strand at 2430.946 kb on + strand at 2430.978 kb on + strand at 2430.978 kb on + strand at 2430.978 kb on + strand at 2430.979 kb on - strand at 2430.979 kb on - strand at 2431.087 kb on - strand, within trpB at 2431.158 kb on - strand, within trpB at 2431.329 kb on + strand, within trpB at 2431.329 kb on + strand, within trpB at 2431.329 kb on + strand, within trpB at 2431.369 kb on + strand, within trpB at 2431.488 kb on + strand, within trpB at 2431.488 kb on + strand, within trpB at 2431.488 kb on + strand, within trpB at 2431.488 kb on + strand, within trpB at 2431.488 kb on + strand, within trpB at 2431.489 kb on - strand, within trpB at 2431.489 kb on - strand, within trpB at 2431.489 kb on - strand, within trpB at 2431.489 kb on - strand, within trpB at 2431.489 kb on - strand, within trpB at 2431.490 kb on + strand, within trpB at 2431.491 kb on - strand, within trpB at 2431.491 kb on - strand, within trpB at 2431.716 kb on - strand, within trpB at 2431.763 kb on + strand, within trpB at 2431.767 kb on + strand, within trpB at 2431.767 kb on + strand, within trpB at 2431.768 kb on - strand, within trpB at 2431.768 kb on - strand, within trpB at 2431.824 kb on + strand, within trpB at 2431.824 kb on + strand, within trpB at 2431.824 kb on + strand, within trpB at 2431.881 kb on - strand, within trpB at 2431.903 kb on - strand, within trpB at 2431.903 kb on - strand, within trpB at 2432.050 kb on + strand at 2432.081 kb on + strand at 2432.082 kb on - strand at 2432.082 kb on - strand at 2432.082 kb on - strand at 2432.083 kb on + strand at 2432.083 kb on + strand at 2432.083 kb on + strand at 2432.083 kb on + strand at 2432.083 kb on + strand at 2432.084 kb on - strand at 2432.084 kb on - strand at 2432.122 kb on + strand at 2432.122 kb on + strand at 2432.123 kb on - strand at 2432.135 kb on + strand at 2432.137 kb on + strand at 2432.145 kb on + strand at 2432.247 kb on + strand, within trpA at 2432.247 kb on + strand, within trpA at 2432.280 kb on + strand, within trpA at 2432.280 kb on + strand, within trpA at 2432.281 kb on - strand, within trpA at 2432.396 kb on - strand, within trpA at 2432.430 kb on - strand, within trpA at 2432.430 kb on - strand, within trpA at 2432.430 kb on - strand, within trpA at 2432.430 kb on - strand, within trpA at 2432.430 kb on - strand, within trpA at 2432.430 kb on - strand, within trpA at 2432.430 kb on - strand, within trpA at 2432.644 kb on + strand, within trpA at 2432.644 kb on + strand, within trpA at 2432.645 kb on - strand, within trpA at 2432.645 kb on - strand, within trpA at 2432.649 kb on - strand, within trpA at 2432.736 kb on + strand, within trpA at 2433.032 kb on + strand at 2433.076 kb on - strand at 2433.077 kb on - strand at 2433.116 kb on + strand at 2433.116 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 2,429,962 + trpCF LU632_RS12765 0.32 -1.3 2,429,988 + trpCF LU632_RS12765 0.34 -2.3 2,429,989 - trpCF LU632_RS12765 0.34 -1.9 2,429,989 - trpCF LU632_RS12765 0.34 -3.0 2,430,030 - trpCF LU632_RS12765 0.37 -0.3 2,430,150 + trpCF LU632_RS12765 0.46 -1.4 2,430,151 - trpCF LU632_RS12765 0.46 -2.1 2,430,178 - trpCF LU632_RS12765 0.48 -0.3 2,430,184 + trpCF LU632_RS12765 0.48 -0.9 2,430,184 + trpCF LU632_RS12765 0.48 -0.9 2,430,298 - trpCF LU632_RS12765 0.57 -1.3 2,430,358 + trpCF LU632_RS12765 0.61 +1.7 2,430,359 - trpCF LU632_RS12765 0.61 -1.3 2,430,441 + trpCF LU632_RS12765 0.67 -0.7 2,430,441 + trpCF LU632_RS12765 0.67 +0.7 2,430,442 - trpCF LU632_RS12765 0.67 -2.3 2,430,442 - trpCF LU632_RS12765 0.67 -0.3 2,430,445 + trpCF LU632_RS12765 0.68 -0.3 2,430,445 + trpCF LU632_RS12765 0.68 -0.3 2,430,446 - trpCF LU632_RS12765 0.68 -0.3 2,430,584 + trpCF LU632_RS12765 0.78 -3.4 2,430,590 + trpCF LU632_RS12765 0.78 -1.3 2,430,609 + trpCF LU632_RS12765 0.80 -0.3 2,430,610 - trpCF LU632_RS12765 0.80 -0.3 2,430,843 + +0.7 2,430,844 - -0.8 2,430,946 + +0.0 2,430,978 + -2.5 2,430,978 + -1.9 2,430,978 + -1.3 2,430,979 - -0.3 2,430,979 - -0.3 2,431,087 - trpB LU632_RS12770 0.13 -0.3 2,431,158 - trpB LU632_RS12770 0.19 -0.3 2,431,329 + trpB LU632_RS12770 0.33 -1.3 2,431,329 + trpB LU632_RS12770 0.33 -1.6 2,431,329 + trpB LU632_RS12770 0.33 -1.1 2,431,369 + trpB LU632_RS12770 0.36 -0.3 2,431,488 + trpB LU632_RS12770 0.46 -0.9 2,431,488 + trpB LU632_RS12770 0.46 -0.7 2,431,488 + trpB LU632_RS12770 0.46 -0.7 2,431,488 + trpB LU632_RS12770 0.46 -1.6 2,431,488 + trpB LU632_RS12770 0.46 -0.3 2,431,489 - trpB LU632_RS12770 0.46 -1.3 2,431,489 - trpB LU632_RS12770 0.46 -1.3 2,431,489 - trpB LU632_RS12770 0.46 -0.3 2,431,489 - trpB LU632_RS12770 0.46 -1.6 2,431,489 - trpB LU632_RS12770 0.46 -1.9 2,431,490 + trpB LU632_RS12770 0.47 -1.3 2,431,491 - trpB LU632_RS12770 0.47 -1.9 2,431,491 - trpB LU632_RS12770 0.47 -1.1 2,431,716 - trpB LU632_RS12770 0.65 +0.3 2,431,763 + trpB LU632_RS12770 0.69 -2.3 2,431,767 + trpB LU632_RS12770 0.70 -2.9 2,431,767 + trpB LU632_RS12770 0.70 -0.3 2,431,768 - trpB LU632_RS12770 0.70 -0.3 2,431,768 - trpB LU632_RS12770 0.70 -3.0 2,431,824 + trpB LU632_RS12770 0.75 -2.6 2,431,824 + trpB LU632_RS12770 0.75 -0.8 2,431,824 + trpB LU632_RS12770 0.75 -0.8 2,431,881 - trpB LU632_RS12770 0.79 -1.3 2,431,903 - trpB LU632_RS12770 0.81 +0.7 2,431,903 - trpB LU632_RS12770 0.81 -1.3 2,432,050 + -2.9 2,432,081 + -0.7 2,432,082 - +0.7 2,432,082 - -1.9 2,432,082 - +0.7 2,432,083 + +0.7 2,432,083 + -0.9 2,432,083 + -0.1 2,432,083 + +0.3 2,432,083 + -0.9 2,432,084 - -0.3 2,432,084 - -0.3 2,432,122 + -0.3 2,432,122 + -1.5 2,432,123 - -0.3 2,432,135 + -2.6 2,432,137 + -0.3 2,432,145 + -1.3 2,432,247 + trpA LU632_RS12775 0.15 -2.3 2,432,247 + trpA LU632_RS12775 0.15 -1.3 2,432,280 + trpA LU632_RS12775 0.19 +0.7 2,432,280 + trpA LU632_RS12775 0.19 -2.2 2,432,281 - trpA LU632_RS12775 0.19 -0.3 2,432,396 - trpA LU632_RS12775 0.33 -0.6 2,432,430 - trpA LU632_RS12775 0.38 +1.3 2,432,430 - trpA LU632_RS12775 0.38 -1.3 2,432,430 - trpA LU632_RS12775 0.38 -1.9 2,432,430 - trpA LU632_RS12775 0.38 -1.3 2,432,430 - trpA LU632_RS12775 0.38 +0.7 2,432,430 - trpA LU632_RS12775 0.38 -3.8 2,432,430 - trpA LU632_RS12775 0.38 -0.3 2,432,644 + trpA LU632_RS12775 0.64 -0.3 2,432,644 + trpA LU632_RS12775 0.64 -1.3 2,432,645 - trpA LU632_RS12775 0.64 -1.9 2,432,645 - trpA LU632_RS12775 0.64 -2.0 2,432,649 - trpA LU632_RS12775 0.65 -1.5 2,432,736 + trpA LU632_RS12775 0.76 -1.3 2,433,032 + -0.6 2,433,076 - -0.7 2,433,077 - -1.5 2,433,116 + -1.4 2,433,116 + -0.6
Or see this region's nucleotide sequence