Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS11500 and LU632_RS11505 are separated by 204 nucleotides LU632_RS11505 and LU632_RS11510 are separated by 105 nucleotides LU632_RS11510 and mpaA are separated by 272 nucleotides mpaA and LU632_RS11520 overlap by 30 nucleotides
LU632_RS11500: LU632_RS11500 - helix-turn-helix domain-containing protein, at 2,192,947 to 2,193,969
_RS11500
LU632_RS11505: LU632_RS11505 - hypothetical protein, at 2,194,174 to 2,194,503
_RS11505
LU632_RS11510: LU632_RS11510 - peptide ABC transporter substrate-binding protein, at 2,194,609 to 2,196,222
_RS11510
LU632_RS11515: mpaA - murein tripeptide amidase MpaA, at 2,196,495 to 2,197,213
mpaA
LU632_RS11520: LU632_RS11520 - L-Ala-D/L-Glu epimerase, at 2,197,184 to 2,197,598
_RS11520
Position (kb)
2194
2195
2196
2197 Strain fitness (log2 ratio)
-2
-1
0
1 at 2194.075 kb on + strand at 2194.114 kb on - strand at 2194.144 kb on + strand at 2194.146 kb on + strand at 2194.146 kb on + strand at 2194.146 kb on + strand at 2194.147 kb on - strand at 2194.269 kb on + strand, within LU632_RS11505 at 2194.269 kb on + strand, within LU632_RS11505 at 2194.465 kb on + strand, within LU632_RS11505 at 2194.466 kb on - strand, within LU632_RS11505 at 2194.483 kb on - strand at 2194.504 kb on - strand at 2194.640 kb on + strand at 2194.666 kb on - strand at 2194.786 kb on - strand, within LU632_RS11510 at 2194.786 kb on - strand, within LU632_RS11510 at 2194.788 kb on + strand, within LU632_RS11510 at 2194.890 kb on + strand, within LU632_RS11510 at 2194.903 kb on - strand, within LU632_RS11510 at 2194.905 kb on - strand, within LU632_RS11510 at 2194.905 kb on - strand, within LU632_RS11510 at 2194.930 kb on + strand, within LU632_RS11510 at 2194.930 kb on + strand, within LU632_RS11510 at 2194.930 kb on + strand, within LU632_RS11510 at 2194.931 kb on - strand, within LU632_RS11510 at 2195.068 kb on - strand, within LU632_RS11510 at 2195.069 kb on + strand, within LU632_RS11510 at 2195.139 kb on + strand, within LU632_RS11510 at 2195.140 kb on - strand, within LU632_RS11510 at 2195.296 kb on + strand, within LU632_RS11510 at 2195.303 kb on - strand, within LU632_RS11510 at 2195.303 kb on - strand, within LU632_RS11510 at 2195.329 kb on - strand, within LU632_RS11510 at 2195.342 kb on + strand, within LU632_RS11510 at 2195.342 kb on + strand, within LU632_RS11510 at 2195.345 kb on + strand, within LU632_RS11510 at 2195.345 kb on + strand, within LU632_RS11510 at 2195.346 kb on - strand, within LU632_RS11510 at 2195.346 kb on - strand, within LU632_RS11510 at 2195.369 kb on + strand, within LU632_RS11510 at 2195.369 kb on + strand, within LU632_RS11510 at 2195.369 kb on + strand, within LU632_RS11510 at 2195.405 kb on + strand, within LU632_RS11510 at 2195.405 kb on + strand, within LU632_RS11510 at 2195.541 kb on + strand, within LU632_RS11510 at 2195.542 kb on - strand, within LU632_RS11510 at 2195.542 kb on - strand, within LU632_RS11510 at 2195.542 kb on - strand, within LU632_RS11510 at 2195.587 kb on - strand, within LU632_RS11510 at 2195.587 kb on - strand, within LU632_RS11510 at 2195.588 kb on + strand, within LU632_RS11510 at 2195.589 kb on - strand, within LU632_RS11510 at 2195.716 kb on + strand, within LU632_RS11510 at 2195.716 kb on + strand, within LU632_RS11510 at 2195.717 kb on - strand, within LU632_RS11510 at 2195.785 kb on - strand, within LU632_RS11510 at 2195.879 kb on - strand, within LU632_RS11510 at 2196.106 kb on - strand at 2196.227 kb on - strand at 2196.228 kb on + strand at 2196.229 kb on - strand at 2196.260 kb on - strand at 2196.310 kb on - strand at 2196.358 kb on + strand at 2196.363 kb on + strand at 2196.378 kb on + strand at 2196.378 kb on + strand at 2196.379 kb on - strand at 2196.379 kb on - strand at 2196.379 kb on - strand at 2196.379 kb on - strand at 2196.824 kb on - strand, within mpaA at 2196.824 kb on - strand, within mpaA at 2196.828 kb on + strand, within mpaA at 2196.828 kb on - strand, within mpaA at 2197.048 kb on - strand, within mpaA at 2197.128 kb on + strand, within mpaA
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 2,194,075 + -1.4 2,194,114 - +0.3 2,194,144 + +0.1 2,194,146 + -0.6 2,194,146 + -0.6 2,194,146 + -0.4 2,194,147 - +0.8 2,194,269 + LU632_RS11505 0.29 +0.3 2,194,269 + LU632_RS11505 0.29 -0.3 2,194,465 + LU632_RS11505 0.88 +0.6 2,194,466 - LU632_RS11505 0.88 +0.8 2,194,483 - +0.2 2,194,504 - +1.0 2,194,640 + -1.4 2,194,666 - -1.1 2,194,786 - LU632_RS11510 0.11 -1.9 2,194,786 - LU632_RS11510 0.11 -2.5 2,194,788 + LU632_RS11510 0.11 -0.5 2,194,890 + LU632_RS11510 0.17 -2.4 2,194,903 - LU632_RS11510 0.18 +1.2 2,194,905 - LU632_RS11510 0.18 -0.7 2,194,905 - LU632_RS11510 0.18 -1.7 2,194,930 + LU632_RS11510 0.20 -1.4 2,194,930 + LU632_RS11510 0.20 -1.4 2,194,930 + LU632_RS11510 0.20 -1.7 2,194,931 - LU632_RS11510 0.20 -1.0 2,195,068 - LU632_RS11510 0.28 -0.4 2,195,069 + LU632_RS11510 0.29 -0.1 2,195,139 + LU632_RS11510 0.33 -1.2 2,195,140 - LU632_RS11510 0.33 -1.4 2,195,296 + LU632_RS11510 0.43 -2.1 2,195,303 - LU632_RS11510 0.43 -0.9 2,195,303 - LU632_RS11510 0.43 -2.4 2,195,329 - LU632_RS11510 0.45 -0.1 2,195,342 + LU632_RS11510 0.45 -1.5 2,195,342 + LU632_RS11510 0.45 -1.5 2,195,345 + LU632_RS11510 0.46 -1.1 2,195,345 + LU632_RS11510 0.46 -0.4 2,195,346 - LU632_RS11510 0.46 -0.6 2,195,346 - LU632_RS11510 0.46 +0.1 2,195,369 + LU632_RS11510 0.47 -1.7 2,195,369 + LU632_RS11510 0.47 -0.9 2,195,369 + LU632_RS11510 0.47 +0.6 2,195,405 + LU632_RS11510 0.49 -1.4 2,195,405 + LU632_RS11510 0.49 -0.4 2,195,541 + LU632_RS11510 0.58 -2.4 2,195,542 - LU632_RS11510 0.58 -0.9 2,195,542 - LU632_RS11510 0.58 -0.7 2,195,542 - LU632_RS11510 0.58 -1.7 2,195,587 - LU632_RS11510 0.61 -2.2 2,195,587 - LU632_RS11510 0.61 -0.5 2,195,588 + LU632_RS11510 0.61 -0.8 2,195,589 - LU632_RS11510 0.61 -0.4 2,195,716 + LU632_RS11510 0.69 -0.4 2,195,716 + LU632_RS11510 0.69 -1.9 2,195,717 - LU632_RS11510 0.69 -0.1 2,195,785 - LU632_RS11510 0.73 -0.9 2,195,879 - LU632_RS11510 0.79 -2.4 2,196,106 - -1.0 2,196,227 - -0.5 2,196,228 + -0.9 2,196,229 - -0.0 2,196,260 - +1.2 2,196,310 - +0.2 2,196,358 + +1.4 2,196,363 + -0.1 2,196,378 + +0.3 2,196,378 + -0.5 2,196,379 - -1.9 2,196,379 - -1.4 2,196,379 - +0.6 2,196,379 - +1.0 2,196,824 - mpaA LU632_RS11515 0.46 +0.1 2,196,824 - mpaA LU632_RS11515 0.46 +0.1 2,196,828 + mpaA LU632_RS11515 0.46 +0.6 2,196,828 - mpaA LU632_RS11515 0.46 +1.4 2,197,048 - mpaA LU632_RS11515 0.77 -0.4 2,197,128 + mpaA LU632_RS11515 0.88 -0.2
Or see this region's nucleotide sequence