Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gsiA and gsiB are separated by 42 nucleotides gsiB and gsiC are separated by 62 nucleotides gsiC and gsiD are separated by 9 nucleotides
LU632_RS09555: gsiA - glutathione ABC transporter ATP-binding protein GsiA, at 1,808,197 to 1,810,059
gsiA
LU632_RS09560: gsiB - glutathione ABC transporter substrate-binding protein GsiB, at 1,810,102 to 1,811,634
gsiB
LU632_RS09565: gsiC - glutathione ABC transporter permease GsiC, at 1,811,697 to 1,812,617
gsiC
LU632_RS09570: gsiD - glutathione ABC transporter permease GsiD, at 1,812,627 to 1,813,532
gsiD
Position (kb)
1810
1811
1812 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1809.342 kb on + strand, within gsiA at 1809.360 kb on + strand, within gsiA at 1809.361 kb on - strand, within gsiA at 1809.361 kb on - strand, within gsiA at 1809.361 kb on - strand, within gsiA at 1809.366 kb on + strand, within gsiA at 1809.430 kb on + strand, within gsiA at 1809.431 kb on - strand, within gsiA at 1809.471 kb on + strand, within gsiA at 1809.471 kb on + strand, within gsiA at 1809.472 kb on - strand, within gsiA at 1809.704 kb on + strand, within gsiA at 1809.705 kb on - strand, within gsiA at 1809.725 kb on + strand, within gsiA at 1809.768 kb on + strand, within gsiA at 1809.768 kb on + strand, within gsiA at 1809.769 kb on - strand, within gsiA at 1809.769 kb on - strand, within gsiA at 1809.770 kb on + strand, within gsiA at 1809.771 kb on - strand, within gsiA at 1809.811 kb on + strand, within gsiA at 1809.811 kb on + strand, within gsiA at 1809.811 kb on + strand, within gsiA at 1809.811 kb on + strand, within gsiA at 1809.811 kb on - strand, within gsiA at 1809.812 kb on - strand, within gsiA at 1809.874 kb on + strand at 1809.888 kb on - strand at 1809.956 kb on + strand at 1809.957 kb on - strand at 1809.957 kb on - strand at 1810.027 kb on + strand at 1810.027 kb on + strand at 1810.027 kb on + strand at 1810.028 kb on - strand at 1810.028 kb on - strand at 1810.028 kb on - strand at 1810.119 kb on + strand at 1810.119 kb on + strand at 1810.119 kb on + strand at 1810.271 kb on - strand, within gsiB at 1810.271 kb on - strand, within gsiB at 1810.271 kb on - strand, within gsiB at 1810.271 kb on - strand, within gsiB at 1810.271 kb on - strand, within gsiB at 1810.299 kb on + strand, within gsiB at 1810.330 kb on + strand, within gsiB at 1810.330 kb on + strand, within gsiB at 1810.331 kb on - strand, within gsiB at 1810.331 kb on - strand, within gsiB at 1810.342 kb on - strand, within gsiB at 1810.353 kb on + strand, within gsiB at 1810.354 kb on - strand, within gsiB at 1810.471 kb on + strand, within gsiB at 1810.472 kb on - strand, within gsiB at 1810.473 kb on + strand, within gsiB at 1810.473 kb on + strand, within gsiB at 1810.474 kb on - strand, within gsiB at 1810.616 kb on - strand, within gsiB at 1810.869 kb on - strand, within gsiB at 1810.874 kb on + strand, within gsiB at 1810.966 kb on + strand, within gsiB at 1810.966 kb on + strand, within gsiB at 1810.966 kb on + strand, within gsiB at 1810.967 kb on - strand, within gsiB at 1811.065 kb on + strand, within gsiB at 1811.080 kb on + strand, within gsiB at 1811.080 kb on + strand, within gsiB at 1811.080 kb on + strand, within gsiB at 1811.081 kb on - strand, within gsiB at 1811.081 kb on - strand, within gsiB at 1811.081 kb on - strand, within gsiB at 1811.081 kb on - strand, within gsiB at 1811.081 kb on - strand, within gsiB at 1811.161 kb on - strand, within gsiB at 1811.411 kb on - strand, within gsiB at 1811.485 kb on + strand at 1811.486 kb on - strand at 1811.486 kb on - strand at 1811.486 kb on - strand at 1811.486 kb on - strand at 1811.487 kb on + strand at 1811.566 kb on - strand at 1811.584 kb on + strand at 1811.585 kb on - strand at 1811.703 kb on - strand at 1811.707 kb on - strand at 1811.712 kb on - strand at 1812.213 kb on + strand, within gsiC at 1812.214 kb on - strand, within gsiC at 1812.215 kb on + strand, within gsiC at 1812.216 kb on - strand, within gsiC at 1812.216 kb on - strand, within gsiC at 1812.216 kb on - strand, within gsiC at 1812.306 kb on + strand, within gsiC at 1812.307 kb on - strand, within gsiC at 1812.307 kb on - strand, within gsiC at 1812.307 kb on - strand, within gsiC at 1812.307 kb on - strand, within gsiC at 1812.308 kb on + strand, within gsiC at 1812.309 kb on - strand, within gsiC at 1812.309 kb on - strand, within gsiC at 1812.452 kb on - strand, within gsiC at 1812.455 kb on + strand, within gsiC at 1812.455 kb on + strand, within gsiC at 1812.455 kb on + strand, within gsiC at 1812.456 kb on - strand, within gsiC at 1812.456 kb on - strand, within gsiC at 1812.530 kb on - strand at 1812.552 kb on - strand at 1812.576 kb on - strand at 1812.582 kb on + strand at 1812.583 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 1,809,342 + gsiA LU632_RS09555 0.61 -1.9 1,809,360 + gsiA LU632_RS09555 0.62 -1.5 1,809,361 - gsiA LU632_RS09555 0.62 -1.6 1,809,361 - gsiA LU632_RS09555 0.62 -0.3 1,809,361 - gsiA LU632_RS09555 0.62 -1.9 1,809,366 + gsiA LU632_RS09555 0.63 -1.4 1,809,430 + gsiA LU632_RS09555 0.66 -0.9 1,809,431 - gsiA LU632_RS09555 0.66 -0.4 1,809,471 + gsiA LU632_RS09555 0.68 -1.3 1,809,471 + gsiA LU632_RS09555 0.68 +0.3 1,809,472 - gsiA LU632_RS09555 0.68 -0.5 1,809,704 + gsiA LU632_RS09555 0.81 -0.1 1,809,705 - gsiA LU632_RS09555 0.81 -1.3 1,809,725 + gsiA LU632_RS09555 0.82 -2.6 1,809,768 + gsiA LU632_RS09555 0.84 -1.9 1,809,768 + gsiA LU632_RS09555 0.84 +1.3 1,809,769 - gsiA LU632_RS09555 0.84 -0.5 1,809,769 - gsiA LU632_RS09555 0.84 -0.7 1,809,770 + gsiA LU632_RS09555 0.84 -0.7 1,809,771 - gsiA LU632_RS09555 0.84 -1.7 1,809,811 + gsiA LU632_RS09555 0.87 -2.3 1,809,811 + gsiA LU632_RS09555 0.87 -1.5 1,809,811 + gsiA LU632_RS09555 0.87 -0.5 1,809,811 + gsiA LU632_RS09555 0.87 +0.3 1,809,811 - gsiA LU632_RS09555 0.87 -1.3 1,809,812 - gsiA LU632_RS09555 0.87 -1.0 1,809,874 + +0.3 1,809,888 - -2.6 1,809,956 + -0.1 1,809,957 - -1.3 1,809,957 - -0.3 1,810,027 + -0.2 1,810,027 + -0.3 1,810,027 + -0.9 1,810,028 - -1.3 1,810,028 - +0.3 1,810,028 - -0.1 1,810,119 + -0.3 1,810,119 + -1.3 1,810,119 + +0.2 1,810,271 - gsiB LU632_RS09560 0.11 -1.2 1,810,271 - gsiB LU632_RS09560 0.11 +1.3 1,810,271 - gsiB LU632_RS09560 0.11 -0.3 1,810,271 - gsiB LU632_RS09560 0.11 -2.3 1,810,271 - gsiB LU632_RS09560 0.11 +1.3 1,810,299 + gsiB LU632_RS09560 0.13 -1.9 1,810,330 + gsiB LU632_RS09560 0.15 -1.0 1,810,330 + gsiB LU632_RS09560 0.15 -0.4 1,810,331 - gsiB LU632_RS09560 0.15 -1.3 1,810,331 - gsiB LU632_RS09560 0.15 -0.3 1,810,342 - gsiB LU632_RS09560 0.16 -1.5 1,810,353 + gsiB LU632_RS09560 0.16 -1.3 1,810,354 - gsiB LU632_RS09560 0.16 -0.3 1,810,471 + gsiB LU632_RS09560 0.24 -1.5 1,810,472 - gsiB LU632_RS09560 0.24 +0.3 1,810,473 + gsiB LU632_RS09560 0.24 +0.7 1,810,473 + gsiB LU632_RS09560 0.24 +0.2 1,810,474 - gsiB LU632_RS09560 0.24 -1.5 1,810,616 - gsiB LU632_RS09560 0.34 +0.5 1,810,869 - gsiB LU632_RS09560 0.50 -1.3 1,810,874 + gsiB LU632_RS09560 0.50 -0.9 1,810,966 + gsiB LU632_RS09560 0.56 -0.6 1,810,966 + gsiB LU632_RS09560 0.56 -0.4 1,810,966 + gsiB LU632_RS09560 0.56 -1.4 1,810,967 - gsiB LU632_RS09560 0.56 -2.3 1,811,065 + gsiB LU632_RS09560 0.63 -0.7 1,811,080 + gsiB LU632_RS09560 0.64 -0.2 1,811,080 + gsiB LU632_RS09560 0.64 -1.8 1,811,080 + gsiB LU632_RS09560 0.64 -1.3 1,811,081 - gsiB LU632_RS09560 0.64 -1.6 1,811,081 - gsiB LU632_RS09560 0.64 -1.0 1,811,081 - gsiB LU632_RS09560 0.64 -0.5 1,811,081 - gsiB LU632_RS09560 0.64 -0.3 1,811,081 - gsiB LU632_RS09560 0.64 -0.7 1,811,161 - gsiB LU632_RS09560 0.69 -2.1 1,811,411 - gsiB LU632_RS09560 0.85 -0.3 1,811,485 + -1.5 1,811,486 - -0.8 1,811,486 - -0.3 1,811,486 - +0.7 1,811,486 - -0.9 1,811,487 + -0.9 1,811,566 - +0.1 1,811,584 + -1.3 1,811,585 - -0.6 1,811,703 - -0.8 1,811,707 - -0.8 1,811,712 - -1.3 1,812,213 + gsiC LU632_RS09565 0.56 +0.1 1,812,214 - gsiC LU632_RS09565 0.56 -3.4 1,812,215 + gsiC LU632_RS09565 0.56 +1.3 1,812,216 - gsiC LU632_RS09565 0.56 -1.7 1,812,216 - gsiC LU632_RS09565 0.56 -0.6 1,812,216 - gsiC LU632_RS09565 0.56 -1.2 1,812,306 + gsiC LU632_RS09565 0.66 +1.3 1,812,307 - gsiC LU632_RS09565 0.66 +0.5 1,812,307 - gsiC LU632_RS09565 0.66 +0.3 1,812,307 - gsiC LU632_RS09565 0.66 +1.3 1,812,307 - gsiC LU632_RS09565 0.66 -2.6 1,812,308 + gsiC LU632_RS09565 0.66 -0.7 1,812,309 - gsiC LU632_RS09565 0.66 -1.3 1,812,309 - gsiC LU632_RS09565 0.66 -0.9 1,812,452 - gsiC LU632_RS09565 0.82 -0.3 1,812,455 + gsiC LU632_RS09565 0.82 -1.3 1,812,455 + gsiC LU632_RS09565 0.82 -1.4 1,812,455 + gsiC LU632_RS09565 0.82 -1.3 1,812,456 - gsiC LU632_RS09565 0.82 -0.3 1,812,456 - gsiC LU632_RS09565 0.82 -0.7 1,812,530 - -1.6 1,812,552 - -1.3 1,812,576 - -0.6 1,812,582 + -0.3 1,812,583 - -1.4
Or see this region's nucleotide sequence