Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisB and hisC overlap by 4 nucleotides hisC and hisD overlap by 4 nucleotides
LU632_RS08935: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 1,686,179 to 1,687,246
hisB
LU632_RS08940: hisC - histidinol-phosphate transaminase, at 1,687,243 to 1,688,334
hisC
LU632_RS08945: hisD - histidinol dehydrogenase, at 1,688,331 to 1,689,638
hisD
Position (kb)
1687
1688
1689 Strain fitness (log2 ratio)
-2
-1
0
1 at 1686.368 kb on + strand, within hisB at 1686.391 kb on + strand, within hisB at 1686.403 kb on + strand, within hisB at 1686.403 kb on + strand, within hisB at 1686.403 kb on + strand, within hisB at 1686.404 kb on - strand, within hisB at 1686.405 kb on + strand, within hisB at 1686.405 kb on + strand, within hisB at 1686.573 kb on + strand, within hisB at 1686.574 kb on - strand, within hisB at 1686.574 kb on - strand, within hisB at 1686.595 kb on + strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.638 kb on - strand, within hisB at 1686.725 kb on - strand, within hisB at 1686.769 kb on + strand, within hisB at 1686.805 kb on - strand, within hisB at 1686.827 kb on - strand, within hisB at 1686.961 kb on + strand, within hisB at 1686.961 kb on + strand, within hisB at 1686.961 kb on + strand, within hisB at 1686.962 kb on - strand, within hisB at 1686.995 kb on - strand, within hisB at 1687.162 kb on + strand at 1687.204 kb on + strand at 1687.380 kb on + strand, within hisC at 1687.380 kb on + strand, within hisC at 1687.442 kb on + strand, within hisC at 1687.442 kb on + strand, within hisC at 1687.442 kb on + strand, within hisC at 1687.442 kb on + strand, within hisC at 1687.442 kb on + strand, within hisC at 1687.442 kb on + strand, within hisC at 1687.443 kb on - strand, within hisC at 1687.443 kb on - strand, within hisC at 1687.443 kb on - strand, within hisC at 1687.443 kb on - strand, within hisC at 1687.521 kb on + strand, within hisC at 1687.527 kb on + strand, within hisC at 1687.676 kb on - strand, within hisC at 1687.711 kb on + strand, within hisC at 1687.716 kb on + strand, within hisC at 1687.774 kb on + strand, within hisC at 1687.774 kb on + strand, within hisC at 1687.774 kb on + strand, within hisC at 1687.777 kb on - strand, within hisC at 1687.777 kb on - strand, within hisC at 1687.778 kb on + strand, within hisC at 1687.778 kb on + strand, within hisC at 1687.778 kb on + strand, within hisC at 1687.778 kb on + strand, within hisC at 1687.778 kb on + strand, within hisC at 1687.778 kb on + strand, within hisC at 1687.779 kb on - strand, within hisC at 1687.886 kb on + strand, within hisC at 1687.958 kb on - strand, within hisC at 1687.958 kb on - strand, within hisC at 1688.000 kb on + strand, within hisC at 1688.000 kb on + strand, within hisC at 1688.001 kb on - strand, within hisC at 1688.001 kb on - strand, within hisC at 1688.005 kb on - strand, within hisC at 1688.129 kb on + strand, within hisC at 1688.242 kb on + strand at 1688.242 kb on + strand at 1688.242 kb on + strand at 1688.242 kb on + strand at 1688.242 kb on + strand at 1688.243 kb on - strand at 1688.243 kb on - strand at 1688.243 kb on - strand at 1688.243 kb on - strand at 1688.286 kb on + strand at 1688.286 kb on + strand at 1688.345 kb on + strand at 1688.346 kb on - strand at 1688.355 kb on - strand at 1688.522 kb on - strand, within hisD at 1688.612 kb on + strand, within hisD at 1688.943 kb on - strand, within hisD at 1688.943 kb on - strand, within hisD at 1688.943 kb on - strand, within hisD at 1688.998 kb on + strand, within hisD at 1688.999 kb on - strand, within hisD at 1689.032 kb on + strand, within hisD at 1689.210 kb on + strand, within hisD at 1689.211 kb on - strand, within hisD at 1689.247 kb on + strand, within hisD at 1689.247 kb on + strand, within hisD at 1689.248 kb on - strand, within hisD at 1689.248 kb on - strand, within hisD
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 1,686,368 + hisB LU632_RS08935 0.18 -0.2 1,686,391 + hisB LU632_RS08935 0.20 -0.2 1,686,403 + hisB LU632_RS08935 0.21 -0.2 1,686,403 + hisB LU632_RS08935 0.21 -1.2 1,686,403 + hisB LU632_RS08935 0.21 +1.4 1,686,404 - hisB LU632_RS08935 0.21 -0.2 1,686,405 + hisB LU632_RS08935 0.21 -0.2 1,686,405 + hisB LU632_RS08935 0.21 -0.2 1,686,573 + hisB LU632_RS08935 0.37 -1.2 1,686,574 - hisB LU632_RS08935 0.37 -1.2 1,686,574 - hisB LU632_RS08935 0.37 -0.2 1,686,595 + hisB LU632_RS08935 0.39 -0.8 1,686,638 - hisB LU632_RS08935 0.43 -0.2 1,686,638 - hisB LU632_RS08935 0.43 -0.2 1,686,638 - hisB LU632_RS08935 0.43 -0.2 1,686,638 - hisB LU632_RS08935 0.43 -0.2 1,686,638 - hisB LU632_RS08935 0.43 -0.2 1,686,638 - hisB LU632_RS08935 0.43 -0.2 1,686,725 - hisB LU632_RS08935 0.51 -0.2 1,686,769 + hisB LU632_RS08935 0.55 +0.8 1,686,805 - hisB LU632_RS08935 0.59 -0.2 1,686,827 - hisB LU632_RS08935 0.61 -0.2 1,686,961 + hisB LU632_RS08935 0.73 -0.2 1,686,961 + hisB LU632_RS08935 0.73 -0.2 1,686,961 + hisB LU632_RS08935 0.73 -0.2 1,686,962 - hisB LU632_RS08935 0.73 -0.2 1,686,995 - hisB LU632_RS08935 0.76 -0.2 1,687,162 + -1.2 1,687,204 + -0.2 1,687,380 + hisC LU632_RS08940 0.13 +0.8 1,687,380 + hisC LU632_RS08940 0.13 -0.2 1,687,442 + hisC LU632_RS08940 0.18 -1.2 1,687,442 + hisC LU632_RS08940 0.18 -0.2 1,687,442 + hisC LU632_RS08940 0.18 -0.2 1,687,442 + hisC LU632_RS08940 0.18 -0.2 1,687,442 + hisC LU632_RS08940 0.18 -0.2 1,687,442 + hisC LU632_RS08940 0.18 -0.2 1,687,443 - hisC LU632_RS08940 0.18 -0.2 1,687,443 - hisC LU632_RS08940 0.18 -0.2 1,687,443 - hisC LU632_RS08940 0.18 -0.6 1,687,443 - hisC LU632_RS08940 0.18 -0.2 1,687,521 + hisC LU632_RS08940 0.25 -0.2 1,687,527 + hisC LU632_RS08940 0.26 -0.2 1,687,676 - hisC LU632_RS08940 0.40 -2.2 1,687,711 + hisC LU632_RS08940 0.43 -0.2 1,687,716 + hisC LU632_RS08940 0.43 -0.2 1,687,774 + hisC LU632_RS08940 0.49 -0.2 1,687,774 + hisC LU632_RS08940 0.49 -0.2 1,687,774 + hisC LU632_RS08940 0.49 -0.2 1,687,777 - hisC LU632_RS08940 0.49 -0.2 1,687,777 - hisC LU632_RS08940 0.49 +0.8 1,687,778 + hisC LU632_RS08940 0.49 -1.2 1,687,778 + hisC LU632_RS08940 0.49 -1.8 1,687,778 + hisC LU632_RS08940 0.49 -0.2 1,687,778 + hisC LU632_RS08940 0.49 +0.8 1,687,778 + hisC LU632_RS08940 0.49 -0.2 1,687,778 + hisC LU632_RS08940 0.49 -0.2 1,687,779 - hisC LU632_RS08940 0.49 -0.2 1,687,886 + hisC LU632_RS08940 0.59 -0.2 1,687,958 - hisC LU632_RS08940 0.65 -0.2 1,687,958 - hisC LU632_RS08940 0.65 -0.2 1,688,000 + hisC LU632_RS08940 0.69 -1.2 1,688,000 + hisC LU632_RS08940 0.69 -0.2 1,688,001 - hisC LU632_RS08940 0.69 -0.2 1,688,001 - hisC LU632_RS08940 0.69 -0.2 1,688,005 - hisC LU632_RS08940 0.70 -1.6 1,688,129 + hisC LU632_RS08940 0.81 +0.8 1,688,242 + +0.4 1,688,242 + -1.2 1,688,242 + -0.2 1,688,242 + -1.2 1,688,242 + -0.2 1,688,243 - -0.2 1,688,243 - +0.4 1,688,243 - -1.2 1,688,243 - -0.2 1,688,286 + -1.2 1,688,286 + -2.2 1,688,345 + -1.2 1,688,346 - +0.9 1,688,355 - +0.8 1,688,522 - hisD LU632_RS08945 0.15 -0.2 1,688,612 + hisD LU632_RS08945 0.21 -0.2 1,688,943 - hisD LU632_RS08945 0.47 -0.2 1,688,943 - hisD LU632_RS08945 0.47 -0.2 1,688,943 - hisD LU632_RS08945 0.47 -0.2 1,688,998 + hisD LU632_RS08945 0.51 -0.2 1,688,999 - hisD LU632_RS08945 0.51 -0.2 1,689,032 + hisD LU632_RS08945 0.54 -0.2 1,689,210 + hisD LU632_RS08945 0.67 -0.2 1,689,211 - hisD LU632_RS08945 0.67 -0.2 1,689,247 + hisD LU632_RS08945 0.70 -0.2 1,689,247 + hisD LU632_RS08945 0.70 -0.2 1,689,248 - hisD LU632_RS08945 0.70 -0.2 1,689,248 - hisD LU632_RS08945 0.70 -0.2
Or see this region's nucleotide sequence