Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt menH and menB are separated by 9 nucleotides menB and menC overlap by 1 nucleotides menC and menE overlap by 13 nucleotides
LU632_RS08110: menH - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 1,518,189 to 1,518,953
menH
LU632_RS08115: menB - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 1,518,963 to 1,519,820
menB
LU632_RS08120: menC - o-succinylbenzoate synthase, at 1,519,820 to 1,520,791
menC
LU632_RS08125: menE - o-succinylbenzoate--CoA ligase, at 1,520,779 to 1,522,149
menE
Position (kb)
1519
1520
1521 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1518.942 kb on - strand at 1518.942 kb on - strand at 1518.942 kb on - strand at 1518.951 kb on + strand at 1519.003 kb on + strand at 1519.243 kb on - strand, within menB at 1519.270 kb on + strand, within menB at 1519.271 kb on - strand, within menB at 1519.271 kb on - strand, within menB at 1519.271 kb on - strand, within menB at 1519.271 kb on - strand, within menB at 1519.271 kb on - strand, within menB at 1519.271 kb on - strand, within menB at 1519.394 kb on + strand, within menB at 1519.439 kb on - strand, within menB at 1519.470 kb on + strand, within menB at 1519.470 kb on + strand, within menB at 1519.470 kb on + strand, within menB at 1519.470 kb on + strand, within menB at 1519.471 kb on - strand, within menB at 1519.471 kb on - strand, within menB at 1519.471 kb on - strand, within menB at 1519.526 kb on - strand, within menB at 1519.530 kb on + strand, within menB at 1519.531 kb on - strand, within menB at 1519.531 kb on - strand, within menB at 1519.735 kb on - strand at 1519.735 kb on - strand at 1519.845 kb on - strand at 1520.231 kb on + strand, within menC at 1520.231 kb on + strand, within menC at 1520.231 kb on + strand, within menC at 1520.232 kb on - strand, within menC at 1520.232 kb on - strand, within menC at 1520.232 kb on - strand, within menC at 1520.232 kb on - strand, within menC at 1520.368 kb on + strand, within menC at 1520.404 kb on + strand, within menC at 1520.778 kb on + strand at 1520.929 kb on + strand, within menE at 1520.930 kb on - strand, within menE at 1520.949 kb on - strand, within menE at 1520.986 kb on + strand, within menE at 1520.997 kb on - strand, within menE at 1521.099 kb on - strand, within menE at 1521.236 kb on + strand, within menE at 1521.237 kb on - strand, within menE at 1521.237 kb on - strand, within menE at 1521.240 kb on + strand, within menE at 1521.240 kb on + strand, within menE at 1521.287 kb on + strand, within menE at 1521.402 kb on + strand, within menE at 1521.403 kb on - strand, within menE at 1521.432 kb on + strand, within menE at 1521.512 kb on + strand, within menE at 1521.513 kb on - strand, within menE at 1521.516 kb on - strand, within menE at 1521.680 kb on + strand, within menE
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 1,518,942 - -1.2 1,518,942 - -0.6 1,518,942 - -0.3 1,518,951 + -1.2 1,519,003 + +0.6 1,519,243 - menB LU632_RS08115 0.33 -0.6 1,519,270 + menB LU632_RS08115 0.36 +1.5 1,519,271 - menB LU632_RS08115 0.36 -1.2 1,519,271 - menB LU632_RS08115 0.36 +0.8 1,519,271 - menB LU632_RS08115 0.36 +1.1 1,519,271 - menB LU632_RS08115 0.36 +0.1 1,519,271 - menB LU632_RS08115 0.36 +0.4 1,519,271 - menB LU632_RS08115 0.36 -1.2 1,519,394 + menB LU632_RS08115 0.50 -0.2 1,519,439 - menB LU632_RS08115 0.55 +0.8 1,519,470 + menB LU632_RS08115 0.59 +0.1 1,519,470 + menB LU632_RS08115 0.59 -0.2 1,519,470 + menB LU632_RS08115 0.59 -1.8 1,519,470 + menB LU632_RS08115 0.59 +0.9 1,519,471 - menB LU632_RS08115 0.59 -0.3 1,519,471 - menB LU632_RS08115 0.59 +1.7 1,519,471 - menB LU632_RS08115 0.59 -0.4 1,519,526 - menB LU632_RS08115 0.66 -0.2 1,519,530 + menB LU632_RS08115 0.66 -0.1 1,519,531 - menB LU632_RS08115 0.66 +0.8 1,519,531 - menB LU632_RS08115 0.66 +0.1 1,519,735 - +0.0 1,519,735 - +0.0 1,519,845 - -0.1 1,520,231 + menC LU632_RS08120 0.42 +0.3 1,520,231 + menC LU632_RS08120 0.42 +0.8 1,520,231 + menC LU632_RS08120 0.42 +3.0 1,520,232 - menC LU632_RS08120 0.42 +0.1 1,520,232 - menC LU632_RS08120 0.42 -0.2 1,520,232 - menC LU632_RS08120 0.42 +0.9 1,520,232 - menC LU632_RS08120 0.42 +1.6 1,520,368 + menC LU632_RS08120 0.56 -0.1 1,520,404 + menC LU632_RS08120 0.60 +0.4 1,520,778 + +0.1 1,520,929 + menE LU632_RS08125 0.11 +0.5 1,520,930 - menE LU632_RS08125 0.11 +0.2 1,520,949 - menE LU632_RS08125 0.12 -1.2 1,520,986 + menE LU632_RS08125 0.15 +0.6 1,520,997 - menE LU632_RS08125 0.16 -0.3 1,521,099 - menE LU632_RS08125 0.23 +0.4 1,521,236 + menE LU632_RS08125 0.33 -0.5 1,521,237 - menE LU632_RS08125 0.33 +0.6 1,521,237 - menE LU632_RS08125 0.33 -0.2 1,521,240 + menE LU632_RS08125 0.34 -0.5 1,521,240 + menE LU632_RS08125 0.34 +0.3 1,521,287 + menE LU632_RS08125 0.37 +0.1 1,521,402 + menE LU632_RS08125 0.45 +0.1 1,521,403 - menE LU632_RS08125 0.46 -0.2 1,521,432 + menE LU632_RS08125 0.48 +1.1 1,521,512 + menE LU632_RS08125 0.53 +0.6 1,521,513 - menE LU632_RS08125 0.54 +0.8 1,521,516 - menE LU632_RS08125 0.54 -0.4 1,521,680 + menE LU632_RS08125 0.66 +0.2
Or see this region's nucleotide sequence