Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt menD and menH overlap by 4 nucleotides menH and menB are separated by 9 nucleotides menB and menC overlap by 1 nucleotides menC and menE overlap by 13 nucleotides
LU632_RS08105: menD - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase, at 1,516,531 to 1,518,192
menD
LU632_RS08110: menH - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 1,518,189 to 1,518,953
menH
LU632_RS08115: menB - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 1,518,963 to 1,519,820
menB
LU632_RS08120: menC - o-succinylbenzoate synthase, at 1,519,820 to 1,520,791
menC
LU632_RS08125: menE - o-succinylbenzoate--CoA ligase, at 1,520,779 to 1,522,149
menE
Position (kb) 1518
1519
1520 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1518.052 kb on + strand at 1518.053 kb on - strand at 1518.053 kb on - strand at 1518.053 kb on - strand at 1518.055 kb on + strand at 1518.140 kb on + strand at 1518.395 kb on - strand, within menH at 1518.395 kb on - strand, within menH at 1518.395 kb on - strand, within menH at 1518.471 kb on + strand, within menH at 1518.472 kb on - strand, within menH at 1518.472 kb on - strand, within menH at 1518.472 kb on - strand, within menH at 1518.472 kb on - strand, within menH at 1518.475 kb on - strand, within menH at 1518.532 kb on - strand, within menH at 1518.548 kb on + strand, within menH at 1518.637 kb on - strand, within menH at 1518.662 kb on + strand, within menH at 1518.942 kb on - strand at 1518.942 kb on - strand at 1518.942 kb on - strand at 1518.951 kb on + strand at 1519.003 kb on + strand at 1519.243 kb on - strand, within menB at 1519.270 kb on + strand, within menB at 1519.271 kb on - strand, within menB at 1519.271 kb on - strand, within menB at 1519.271 kb on - strand, within menB at 1519.271 kb on - strand, within menB at 1519.271 kb on - strand, within menB at 1519.271 kb on - strand, within menB at 1519.394 kb on + strand, within menB at 1519.439 kb on - strand, within menB at 1519.470 kb on + strand, within menB at 1519.470 kb on + strand, within menB at 1519.470 kb on + strand, within menB at 1519.470 kb on + strand, within menB at 1519.471 kb on - strand, within menB at 1519.471 kb on - strand, within menB at 1519.471 kb on - strand, within menB at 1519.526 kb on - strand, within menB at 1519.530 kb on + strand, within menB at 1519.531 kb on - strand, within menB at 1519.531 kb on - strand, within menB at 1519.735 kb on - strand at 1519.735 kb on - strand at 1519.845 kb on - strand at 1520.231 kb on + strand, within menC at 1520.231 kb on + strand, within menC at 1520.231 kb on + strand, within menC at 1520.232 kb on - strand, within menC at 1520.232 kb on - strand, within menC at 1520.232 kb on - strand, within menC at 1520.232 kb on - strand, within menC at 1520.368 kb on + strand, within menC at 1520.404 kb on + strand, within menC at 1520.778 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 1,518,052 + -0.4 1,518,053 - -0.2 1,518,053 - -0.2 1,518,053 - +1.1 1,518,055 + +0.5 1,518,140 + -1.5 1,518,395 - menH LU632_RS08110 0.27 -0.1 1,518,395 - menH LU632_RS08110 0.27 +0.4 1,518,395 - menH LU632_RS08110 0.27 +1.0 1,518,471 + menH LU632_RS08110 0.37 -0.2 1,518,472 - menH LU632_RS08110 0.37 -1.2 1,518,472 - menH LU632_RS08110 0.37 +0.5 1,518,472 - menH LU632_RS08110 0.37 +1.6 1,518,472 - menH LU632_RS08110 0.37 +0.2 1,518,475 - menH LU632_RS08110 0.37 -0.3 1,518,532 - menH LU632_RS08110 0.45 +0.7 1,518,548 + menH LU632_RS08110 0.47 +0.4 1,518,637 - menH LU632_RS08110 0.59 +0.5 1,518,662 + menH LU632_RS08110 0.62 -0.9 1,518,942 - -1.2 1,518,942 - -0.6 1,518,942 - -0.3 1,518,951 + -1.2 1,519,003 + +0.6 1,519,243 - menB LU632_RS08115 0.33 -0.6 1,519,270 + menB LU632_RS08115 0.36 +1.5 1,519,271 - menB LU632_RS08115 0.36 -1.2 1,519,271 - menB LU632_RS08115 0.36 +0.8 1,519,271 - menB LU632_RS08115 0.36 +1.1 1,519,271 - menB LU632_RS08115 0.36 +0.1 1,519,271 - menB LU632_RS08115 0.36 +0.4 1,519,271 - menB LU632_RS08115 0.36 -1.2 1,519,394 + menB LU632_RS08115 0.50 -0.2 1,519,439 - menB LU632_RS08115 0.55 +0.8 1,519,470 + menB LU632_RS08115 0.59 +0.1 1,519,470 + menB LU632_RS08115 0.59 -0.2 1,519,470 + menB LU632_RS08115 0.59 -1.8 1,519,470 + menB LU632_RS08115 0.59 +0.9 1,519,471 - menB LU632_RS08115 0.59 -0.3 1,519,471 - menB LU632_RS08115 0.59 +1.7 1,519,471 - menB LU632_RS08115 0.59 -0.4 1,519,526 - menB LU632_RS08115 0.66 -0.2 1,519,530 + menB LU632_RS08115 0.66 -0.1 1,519,531 - menB LU632_RS08115 0.66 +0.8 1,519,531 - menB LU632_RS08115 0.66 +0.1 1,519,735 - +0.0 1,519,735 - +0.0 1,519,845 - -0.1 1,520,231 + menC LU632_RS08120 0.42 +0.3 1,520,231 + menC LU632_RS08120 0.42 +0.8 1,520,231 + menC LU632_RS08120 0.42 +3.0 1,520,232 - menC LU632_RS08120 0.42 +0.1 1,520,232 - menC LU632_RS08120 0.42 -0.2 1,520,232 - menC LU632_RS08120 0.42 +0.9 1,520,232 - menC LU632_RS08120 0.42 +1.6 1,520,368 + menC LU632_RS08120 0.56 -0.1 1,520,404 + menC LU632_RS08120 0.60 +0.4 1,520,778 + +0.1
Or see this region's nucleotide sequence