Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoG and nuoH overlap by 4 nucleotides nuoH and nuoI are separated by 13 nucleotides nuoI and nuoJ are separated by 10 nucleotides nuoJ and nuoK overlap by 4 nucleotides nuoK and nuoL overlap by 4 nucleotides
LU632_RS07990: nuoG - NADH-quinone oxidoreductase subunit NuoG, at 1,496,288 to 1,499,014
nuoG
LU632_RS07995: nuoH - NADH-quinone oxidoreductase subunit NuoH, at 1,499,011 to 1,499,988
nuoH
LU632_RS08000: nuoI - NADH-quinone oxidoreductase subunit NuoI, at 1,500,002 to 1,500,544
nuoI
LU632_RS08005: nuoJ - NADH-quinone oxidoreductase subunit J, at 1,500,555 to 1,501,103
nuoJ
LU632_RS08010: nuoK - NADH-quinone oxidoreductase subunit NuoK, at 1,501,100 to 1,501,402
nuoK
LU632_RS08015: nuoL - NADH-quinone oxidoreductase subunit L, at 1,501,399 to 1,503,237
nuoL
Position (kb)
1500
1501 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1499.066 kb on + strand at 1499.346 kb on + strand, within nuoH at 1499.346 kb on + strand, within nuoH at 1499.392 kb on + strand, within nuoH at 1499.431 kb on + strand, within nuoH at 1499.432 kb on - strand, within nuoH at 1499.442 kb on - strand, within nuoH at 1499.734 kb on + strand, within nuoH at 1499.735 kb on - strand, within nuoH at 1499.735 kb on - strand, within nuoH at 1499.996 kb on + strand at 1499.997 kb on - strand at 1500.010 kb on - strand at 1500.054 kb on + strand at 1500.054 kb on + strand at 1500.224 kb on - strand, within nuoI at 1500.349 kb on + strand, within nuoI at 1500.395 kb on + strand, within nuoI at 1500.395 kb on - strand, within nuoI at 1500.396 kb on - strand, within nuoI at 1500.396 kb on - strand, within nuoI at 1500.396 kb on - strand, within nuoI at 1500.445 kb on + strand, within nuoI at 1500.446 kb on - strand, within nuoI at 1500.446 kb on - strand, within nuoI at 1500.537 kb on + strand at 1500.537 kb on + strand at 1500.643 kb on + strand, within nuoJ at 1500.643 kb on + strand, within nuoJ at 1500.643 kb on + strand, within nuoJ at 1500.643 kb on + strand, within nuoJ at 1500.643 kb on + strand, within nuoJ at 1500.643 kb on + strand, within nuoJ at 1500.644 kb on - strand, within nuoJ at 1500.644 kb on - strand, within nuoJ at 1500.644 kb on - strand, within nuoJ at 1500.645 kb on + strand, within nuoJ at 1500.645 kb on + strand, within nuoJ at 1500.645 kb on + strand, within nuoJ at 1500.646 kb on - strand, within nuoJ at 1500.879 kb on + strand, within nuoJ at 1500.880 kb on - strand, within nuoJ at 1500.964 kb on - strand, within nuoJ at 1501.020 kb on + strand, within nuoJ at 1501.021 kb on - strand, within nuoJ at 1501.190 kb on - strand, within nuoK at 1501.283 kb on + strand, within nuoK at 1501.283 kb on + strand, within nuoK at 1501.283 kb on + strand, within nuoK at 1501.283 kb on + strand, within nuoK at 1501.283 kb on + strand, within nuoK at 1501.283 kb on + strand, within nuoK at 1501.283 kb on + strand, within nuoK at 1501.283 kb on + strand, within nuoK at 1501.284 kb on - strand, within nuoK at 1501.284 kb on - strand, within nuoK at 1501.284 kb on - strand, within nuoK at 1501.533 kb on + strand at 1501.533 kb on + strand at 1501.533 kb on + strand at 1501.534 kb on - strand at 1501.537 kb on + strand at 1501.537 kb on + strand at 1501.538 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 1,499,066 + -0.2 1,499,346 + nuoH LU632_RS07995 0.34 -2.2 1,499,346 + nuoH LU632_RS07995 0.34 -1.2 1,499,392 + nuoH LU632_RS07995 0.39 -2.8 1,499,431 + nuoH LU632_RS07995 0.43 -3.0 1,499,432 - nuoH LU632_RS07995 0.43 -1.8 1,499,442 - nuoH LU632_RS07995 0.44 -1.8 1,499,734 + nuoH LU632_RS07995 0.74 -4.2 1,499,735 - nuoH LU632_RS07995 0.74 -4.4 1,499,735 - nuoH LU632_RS07995 0.74 -0.2 1,499,996 + +0.4 1,499,997 - -3.7 1,500,010 - -1.8 1,500,054 + -1.8 1,500,054 + -4.1 1,500,224 - nuoI LU632_RS08000 0.41 -3.7 1,500,349 + nuoI LU632_RS08000 0.64 -1.2 1,500,395 + nuoI LU632_RS08000 0.72 -2.2 1,500,395 - nuoI LU632_RS08000 0.72 -1.2 1,500,396 - nuoI LU632_RS08000 0.73 -3.2 1,500,396 - nuoI LU632_RS08000 0.73 -0.2 1,500,396 - nuoI LU632_RS08000 0.73 -1.2 1,500,445 + nuoI LU632_RS08000 0.82 -1.8 1,500,446 - nuoI LU632_RS08000 0.82 -3.4 1,500,446 - nuoI LU632_RS08000 0.82 -2.5 1,500,537 + -0.8 1,500,537 + -0.2 1,500,643 + nuoJ LU632_RS08005 0.16 -0.2 1,500,643 + nuoJ LU632_RS08005 0.16 -1.2 1,500,643 + nuoJ LU632_RS08005 0.16 -1.8 1,500,643 + nuoJ LU632_RS08005 0.16 -2.5 1,500,643 + nuoJ LU632_RS08005 0.16 -1.8 1,500,643 + nuoJ LU632_RS08005 0.16 -0.2 1,500,644 - nuoJ LU632_RS08005 0.16 -5.1 1,500,644 - nuoJ LU632_RS08005 0.16 -0.2 1,500,644 - nuoJ LU632_RS08005 0.16 -1.8 1,500,645 + nuoJ LU632_RS08005 0.16 -3.4 1,500,645 + nuoJ LU632_RS08005 0.16 -1.2 1,500,645 + nuoJ LU632_RS08005 0.16 -1.2 1,500,646 - nuoJ LU632_RS08005 0.17 -1.8 1,500,879 + nuoJ LU632_RS08005 0.59 -1.5 1,500,880 - nuoJ LU632_RS08005 0.59 -2.5 1,500,964 - nuoJ LU632_RS08005 0.74 -3.7 1,501,020 + nuoJ LU632_RS08005 0.85 -2.2 1,501,021 - nuoJ LU632_RS08005 0.85 -0.2 1,501,190 - nuoK LU632_RS08010 0.30 -2.5 1,501,283 + nuoK LU632_RS08010 0.60 -0.2 1,501,283 + nuoK LU632_RS08010 0.60 -1.2 1,501,283 + nuoK LU632_RS08010 0.60 -0.2 1,501,283 + nuoK LU632_RS08010 0.60 -3.2 1,501,283 + nuoK LU632_RS08010 0.60 -0.2 1,501,283 + nuoK LU632_RS08010 0.60 -0.2 1,501,283 + nuoK LU632_RS08010 0.60 -2.2 1,501,283 + nuoK LU632_RS08010 0.60 -2.0 1,501,284 - nuoK LU632_RS08010 0.61 -2.2 1,501,284 - nuoK LU632_RS08010 0.61 -1.8 1,501,284 - nuoK LU632_RS08010 0.61 -2.8 1,501,533 + -0.2 1,501,533 + -0.2 1,501,533 + -2.5 1,501,534 - -3.8 1,501,537 + -0.2 1,501,537 + -1.8 1,501,538 - -0.2
Or see this region's nucleotide sequence