Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoC and nuoE are separated by 2 nucleotides nuoE and nuoF overlap by 4 nucleotides nuoF and nuoG are separated by 114 nucleotides
LU632_RS07975: nuoC - NADH-quinone oxidoreductase subunit C/D, at 1,492,513 to 1,494,312
nuoC
LU632_RS07980: nuoE - NADH-quinone oxidoreductase subunit NuoE, at 1,494,315 to 1,494,830
nuoE
LU632_RS07985: nuoF - NADH-quinone oxidoreductase subunit NuoF, at 1,494,827 to 1,496,173
nuoF
LU632_RS07990: nuoG - NADH-quinone oxidoreductase subunit NuoG, at 1,496,288 to 1,499,014
nuoG
Position (kb)
1494
1495
1496
1497 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1494.009 kb on + strand, within nuoC at 1494.145 kb on + strand at 1494.146 kb on - strand at 1494.146 kb on - strand at 1494.148 kb on + strand at 1494.196 kb on - strand at 1494.269 kb on - strand at 1494.314 kb on + strand at 1494.315 kb on - strand at 1494.708 kb on - strand, within nuoE at 1494.773 kb on + strand, within nuoE at 1494.850 kb on + strand at 1495.127 kb on + strand, within nuoF at 1495.128 kb on - strand, within nuoF at 1495.128 kb on - strand, within nuoF at 1495.130 kb on - strand, within nuoF at 1495.218 kb on - strand, within nuoF at 1495.218 kb on - strand, within nuoF at 1495.218 kb on - strand, within nuoF at 1495.219 kb on + strand, within nuoF at 1495.219 kb on + strand, within nuoF at 1495.220 kb on - strand, within nuoF at 1495.220 kb on - strand, within nuoF at 1495.220 kb on - strand, within nuoF at 1495.239 kb on - strand, within nuoF at 1495.296 kb on - strand, within nuoF at 1495.364 kb on + strand, within nuoF at 1495.547 kb on - strand, within nuoF at 1495.661 kb on + strand, within nuoF at 1495.662 kb on - strand, within nuoF at 1495.662 kb on - strand, within nuoF at 1495.894 kb on + strand, within nuoF at 1495.987 kb on - strand, within nuoF at 1496.072 kb on + strand at 1496.073 kb on - strand at 1496.191 kb on + strand at 1496.231 kb on - strand at 1496.231 kb on - strand at 1496.233 kb on + strand at 1496.241 kb on - strand at 1496.288 kb on - strand at 1496.294 kb on - strand at 1496.294 kb on - strand at 1496.304 kb on + strand at 1496.305 kb on - strand at 1496.488 kb on + strand at 1496.515 kb on + strand at 1496.515 kb on + strand at 1496.522 kb on - strand at 1496.826 kb on - strand, within nuoG at 1496.939 kb on - strand, within nuoG at 1496.955 kb on - strand, within nuoG at 1496.996 kb on - strand, within nuoG at 1497.058 kb on - strand, within nuoG at 1497.058 kb on - strand, within nuoG
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 1,494,009 + nuoC LU632_RS07975 0.83 -1.2 1,494,145 + -0.2 1,494,146 - -3.9 1,494,146 - -2.2 1,494,148 + -2.5 1,494,196 - -1.2 1,494,269 - -1.2 1,494,314 + -1.8 1,494,315 - -2.2 1,494,708 - nuoE LU632_RS07980 0.76 -3.0 1,494,773 + nuoE LU632_RS07980 0.89 -3.8 1,494,850 + -1.2 1,495,127 + nuoF LU632_RS07985 0.22 -0.2 1,495,128 - nuoF LU632_RS07985 0.22 -2.5 1,495,128 - nuoF LU632_RS07985 0.22 -2.8 1,495,130 - nuoF LU632_RS07985 0.22 -2.5 1,495,218 - nuoF LU632_RS07985 0.29 -3.2 1,495,218 - nuoF LU632_RS07985 0.29 -2.5 1,495,218 - nuoF LU632_RS07985 0.29 -3.0 1,495,219 + nuoF LU632_RS07985 0.29 -1.2 1,495,219 + nuoF LU632_RS07985 0.29 -1.2 1,495,220 - nuoF LU632_RS07985 0.29 -3.0 1,495,220 - nuoF LU632_RS07985 0.29 -4.3 1,495,220 - nuoF LU632_RS07985 0.29 -0.2 1,495,239 - nuoF LU632_RS07985 0.31 -4.3 1,495,296 - nuoF LU632_RS07985 0.35 -4.8 1,495,364 + nuoF LU632_RS07985 0.40 -2.5 1,495,547 - nuoF LU632_RS07985 0.53 -4.0 1,495,661 + nuoF LU632_RS07985 0.62 -1.2 1,495,662 - nuoF LU632_RS07985 0.62 -2.2 1,495,662 - nuoF LU632_RS07985 0.62 -1.2 1,495,894 + nuoF LU632_RS07985 0.79 -2.5 1,495,987 - nuoF LU632_RS07985 0.86 -2.2 1,496,072 + -0.2 1,496,073 - -1.2 1,496,191 + +2.1 1,496,231 - -2.2 1,496,231 - -1.8 1,496,233 + +0.6 1,496,241 - -2.8 1,496,288 - -1.8 1,496,294 - -4.0 1,496,294 - -1.2 1,496,304 + -1.2 1,496,305 - -0.2 1,496,488 + -1.2 1,496,515 + -3.0 1,496,515 + -2.5 1,496,522 - -2.8 1,496,826 - nuoG LU632_RS07990 0.20 -0.2 1,496,939 - nuoG LU632_RS07990 0.24 -2.2 1,496,955 - nuoG LU632_RS07990 0.24 -1.2 1,496,996 - nuoG LU632_RS07990 0.26 -1.2 1,497,058 - nuoG LU632_RS07990 0.28 -0.7 1,497,058 - nuoG LU632_RS07990 0.28 -1.8
Or see this region's nucleotide sequence