Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS06355 and mazG are separated by 2 nucleotides mazG and pyrG are separated by 211 nucleotides pyrG and eno are separated by 76 nucleotides
LU632_RS06355: LU632_RS06355 - hypothetical protein, at 1,221,779 to 1,221,997
_RS06355
LU632_RS06360: mazG - nucleoside triphosphate pyrophosphohydrolase, at 1,222,000 to 1,222,782
mazG
LU632_RS06365: pyrG - CTP synthase (glutamine hydrolyzing), at 1,222,994 to 1,224,631
pyrG
LU632_RS06370: eno - phosphopyruvate hydratase, at 1,224,708 to 1,226,003
eno
Position (kb)
1222
1223
1224
1225 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1222.084 kb on + strand, within mazG at 1222.084 kb on + strand, within mazG at 1222.085 kb on - strand, within mazG at 1222.085 kb on - strand, within mazG at 1222.187 kb on + strand, within mazG at 1222.188 kb on - strand, within mazG at 1222.204 kb on + strand, within mazG at 1222.213 kb on - strand, within mazG at 1222.236 kb on + strand, within mazG at 1222.255 kb on - strand, within mazG at 1222.293 kb on + strand, within mazG at 1222.294 kb on - strand, within mazG at 1222.403 kb on + strand, within mazG at 1222.403 kb on + strand, within mazG at 1222.404 kb on - strand, within mazG at 1222.404 kb on - strand, within mazG at 1222.410 kb on + strand, within mazG at 1222.508 kb on - strand, within mazG at 1222.508 kb on - strand, within mazG at 1222.627 kb on - strand, within mazG at 1222.627 kb on - strand, within mazG at 1222.630 kb on - strand, within mazG at 1222.781 kb on - strand at 1222.781 kb on - strand at 1222.798 kb on - strand at 1222.936 kb on + strand at 1224.692 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 1,222,084 + mazG LU632_RS06360 0.11 +0.5 1,222,084 + mazG LU632_RS06360 0.11 +0.3 1,222,085 - mazG LU632_RS06360 0.11 -0.0 1,222,085 - mazG LU632_RS06360 0.11 +0.1 1,222,187 + mazG LU632_RS06360 0.24 -0.2 1,222,188 - mazG LU632_RS06360 0.24 +1.4 1,222,204 + mazG LU632_RS06360 0.26 +1.2 1,222,213 - mazG LU632_RS06360 0.27 +0.6 1,222,236 + mazG LU632_RS06360 0.30 +0.1 1,222,255 - mazG LU632_RS06360 0.33 -0.9 1,222,293 + mazG LU632_RS06360 0.37 +0.8 1,222,294 - mazG LU632_RS06360 0.38 -0.2 1,222,403 + mazG LU632_RS06360 0.51 -0.2 1,222,403 + mazG LU632_RS06360 0.51 -0.7 1,222,404 - mazG LU632_RS06360 0.52 +2.1 1,222,404 - mazG LU632_RS06360 0.52 -1.0 1,222,410 + mazG LU632_RS06360 0.52 +0.0 1,222,508 - mazG LU632_RS06360 0.65 +0.2 1,222,508 - mazG LU632_RS06360 0.65 -0.2 1,222,627 - mazG LU632_RS06360 0.80 -0.5 1,222,627 - mazG LU632_RS06360 0.80 +0.9 1,222,630 - mazG LU632_RS06360 0.80 +0.1 1,222,781 - +1.2 1,222,781 - +2.8 1,222,798 - -0.6 1,222,936 + -0.2 1,224,692 + +0.8
Or see this region's nucleotide sequence