Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rlmM and xni are separated by 34 nucleotides xni and ppnN are separated by 42 nucleotides ppnN and queF are separated by 190 nucleotides
LU632_RS06265: rlmM - 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM, at 1,199,076 to 1,200,176
rlmM
LU632_RS06270: xni - flap endonuclease Xni, at 1,200,211 to 1,200,975
xni
LU632_RS06275: ppnN - nucleotide 5'-monophosphate nucleosidase PpnN, at 1,201,018 to 1,202,376
ppnN
LU632_RS06280: queF - NADPH-dependent 7-cyano-7-deazaguanine reductase QueF, at 1,202,567 to 1,203,412
queF
Position (kb)
1201
1202
1203 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1200.099 kb on + strand at 1200.100 kb on - strand at 1200.100 kb on - strand at 1200.191 kb on + strand at 1200.192 kb on - strand at 1200.210 kb on - strand at 1200.298 kb on - strand, within xni at 1200.474 kb on - strand, within xni at 1200.556 kb on + strand, within xni at 1200.561 kb on + strand, within xni at 1200.594 kb on + strand, within xni at 1200.779 kb on + strand, within xni at 1200.779 kb on + strand, within xni at 1200.779 kb on + strand, within xni at 1200.780 kb on - strand, within xni at 1200.942 kb on + strand at 1200.943 kb on - strand at 1200.959 kb on - strand at 1200.962 kb on - strand at 1201.046 kb on + strand at 1201.093 kb on + strand at 1201.094 kb on - strand at 1201.097 kb on - strand at 1201.118 kb on - strand at 1201.335 kb on + strand, within ppnN at 1201.375 kb on - strand, within ppnN at 1201.440 kb on - strand, within ppnN at 1201.440 kb on - strand, within ppnN at 1201.468 kb on - strand, within ppnN at 1201.482 kb on + strand, within ppnN at 1201.483 kb on - strand, within ppnN at 1201.499 kb on + strand, within ppnN at 1201.499 kb on + strand, within ppnN at 1201.499 kb on + strand, within ppnN at 1201.500 kb on - strand, within ppnN at 1201.583 kb on + strand, within ppnN at 1201.627 kb on - strand, within ppnN at 1201.646 kb on - strand, within ppnN at 1201.646 kb on - strand, within ppnN at 1201.646 kb on - strand, within ppnN at 1201.765 kb on - strand, within ppnN at 1201.766 kb on + strand, within ppnN at 1201.837 kb on - strand, within ppnN at 1201.842 kb on + strand, within ppnN at 1201.842 kb on + strand, within ppnN at 1201.843 kb on - strand, within ppnN at 1201.893 kb on - strand, within ppnN at 1202.057 kb on - strand, within ppnN at 1202.078 kb on - strand, within ppnN at 1202.109 kb on + strand, within ppnN at 1202.182 kb on + strand, within ppnN at 1202.251 kb on - strand at 1202.388 kb on + strand at 1202.389 kb on - strand at 1202.431 kb on + strand at 1202.432 kb on - strand at 1202.455 kb on + strand at 1202.456 kb on - strand at 1202.456 kb on - strand at 1202.457 kb on + strand at 1202.457 kb on + strand at 1202.462 kb on - strand at 1202.515 kb on - strand at 1202.521 kb on - strand at 1202.525 kb on + strand at 1202.526 kb on - strand at 1202.526 kb on - strand at 1202.599 kb on + strand at 1202.600 kb on - strand at 1202.600 kb on - strand at 1202.650 kb on + strand at 1202.651 kb on - strand at 1202.651 kb on - strand at 1202.652 kb on + strand, within queF at 1202.652 kb on + strand, within queF at 1202.653 kb on - strand, within queF at 1202.662 kb on - strand, within queF at 1202.662 kb on - strand, within queF at 1202.662 kb on - strand, within queF at 1202.698 kb on + strand, within queF at 1202.807 kb on + strand, within queF at 1202.893 kb on - strand, within queF at 1202.893 kb on - strand, within queF at 1202.934 kb on + strand, within queF at 1202.934 kb on + strand, within queF at 1202.935 kb on - strand, within queF at 1202.935 kb on - strand, within queF at 1202.935 kb on - strand, within queF at 1202.935 kb on - strand, within queF at 1202.935 kb on - strand, within queF at 1202.965 kb on + strand, within queF at 1202.966 kb on - strand, within queF at 1202.966 kb on - strand, within queF at 1203.003 kb on - strand, within queF at 1203.118 kb on + strand, within queF at 1203.203 kb on + strand, within queF at 1203.204 kb on - strand, within queF at 1203.235 kb on - strand, within queF at 1203.327 kb on - strand, within queF at 1203.339 kb on + strand at 1203.340 kb on + strand at 1203.340 kb on - strand at 1203.340 kb on - strand at 1203.343 kb on + strand at 1203.352 kb on - strand at 1203.352 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 1,200,099 + +0.4 1,200,100 - +2.1 1,200,100 - +0.5 1,200,191 + -0.7 1,200,192 - +0.1 1,200,210 - +0.8 1,200,298 - xni LU632_RS06270 0.11 +0.6 1,200,474 - xni LU632_RS06270 0.34 +0.6 1,200,556 + xni LU632_RS06270 0.45 -1.2 1,200,561 + xni LU632_RS06270 0.46 -2.5 1,200,594 + xni LU632_RS06270 0.50 -0.2 1,200,779 + xni LU632_RS06270 0.74 +0.3 1,200,779 + xni LU632_RS06270 0.74 -0.5 1,200,779 + xni LU632_RS06270 0.74 +0.1 1,200,780 - xni LU632_RS06270 0.74 +1.9 1,200,942 + +0.2 1,200,943 - -0.1 1,200,959 - +0.8 1,200,962 - +0.9 1,201,046 + -1.1 1,201,093 + -0.5 1,201,094 - -0.7 1,201,097 - -2.0 1,201,118 - -2.5 1,201,335 + ppnN LU632_RS06275 0.23 -2.2 1,201,375 - ppnN LU632_RS06275 0.26 -1.0 1,201,440 - ppnN LU632_RS06275 0.31 +0.1 1,201,440 - ppnN LU632_RS06275 0.31 -2.5 1,201,468 - ppnN LU632_RS06275 0.33 -1.2 1,201,482 + ppnN LU632_RS06275 0.34 -0.8 1,201,483 - ppnN LU632_RS06275 0.34 -1.1 1,201,499 + ppnN LU632_RS06275 0.35 -1.2 1,201,499 + ppnN LU632_RS06275 0.35 -3.8 1,201,499 + ppnN LU632_RS06275 0.35 -2.6 1,201,500 - ppnN LU632_RS06275 0.35 -1.3 1,201,583 + ppnN LU632_RS06275 0.42 -0.8 1,201,627 - ppnN LU632_RS06275 0.45 -1.5 1,201,646 - ppnN LU632_RS06275 0.46 -1.8 1,201,646 - ppnN LU632_RS06275 0.46 -0.8 1,201,646 - ppnN LU632_RS06275 0.46 -0.2 1,201,765 - ppnN LU632_RS06275 0.55 -2.3 1,201,766 + ppnN LU632_RS06275 0.55 -0.6 1,201,837 - ppnN LU632_RS06275 0.60 -1.4 1,201,842 + ppnN LU632_RS06275 0.61 -1.2 1,201,842 + ppnN LU632_RS06275 0.61 -1.3 1,201,843 - ppnN LU632_RS06275 0.61 -1.5 1,201,893 - ppnN LU632_RS06275 0.64 -2.8 1,202,057 - ppnN LU632_RS06275 0.76 -1.2 1,202,078 - ppnN LU632_RS06275 0.78 -1.8 1,202,109 + ppnN LU632_RS06275 0.80 -1.8 1,202,182 + ppnN LU632_RS06275 0.86 -1.8 1,202,251 - -1.8 1,202,388 + -1.5 1,202,389 - -0.5 1,202,431 + -0.2 1,202,432 - +0.2 1,202,455 + -0.3 1,202,456 - +1.8 1,202,456 - -0.5 1,202,457 + -0.2 1,202,457 + -1.2 1,202,462 - -1.2 1,202,515 - +1.2 1,202,521 - +0.8 1,202,525 + -2.2 1,202,526 - -1.2 1,202,526 - +0.3 1,202,599 + +1.8 1,202,600 - +0.1 1,202,600 - -0.2 1,202,650 + -0.2 1,202,651 - -0.2 1,202,651 - +0.7 1,202,652 + queF LU632_RS06280 0.10 -0.6 1,202,652 + queF LU632_RS06280 0.10 -0.4 1,202,653 - queF LU632_RS06280 0.10 +0.1 1,202,662 - queF LU632_RS06280 0.11 +2.6 1,202,662 - queF LU632_RS06280 0.11 +0.0 1,202,662 - queF LU632_RS06280 0.11 +1.1 1,202,698 + queF LU632_RS06280 0.15 -0.2 1,202,807 + queF LU632_RS06280 0.28 -0.2 1,202,893 - queF LU632_RS06280 0.39 -0.8 1,202,893 - queF LU632_RS06280 0.39 +0.1 1,202,934 + queF LU632_RS06280 0.43 +0.1 1,202,934 + queF LU632_RS06280 0.43 -0.0 1,202,935 - queF LU632_RS06280 0.43 -0.4 1,202,935 - queF LU632_RS06280 0.43 -0.2 1,202,935 - queF LU632_RS06280 0.43 +0.3 1,202,935 - queF LU632_RS06280 0.43 +0.8 1,202,935 - queF LU632_RS06280 0.43 -0.2 1,202,965 + queF LU632_RS06280 0.47 -2.2 1,202,966 - queF LU632_RS06280 0.47 +0.2 1,202,966 - queF LU632_RS06280 0.47 -0.5 1,203,003 - queF LU632_RS06280 0.52 -0.2 1,203,118 + queF LU632_RS06280 0.65 +0.3 1,203,203 + queF LU632_RS06280 0.75 +0.5 1,203,204 - queF LU632_RS06280 0.75 +0.2 1,203,235 - queF LU632_RS06280 0.79 +0.5 1,203,327 - queF LU632_RS06280 0.90 +1.6 1,203,339 + -1.2 1,203,340 + +0.3 1,203,340 - +0.0 1,203,340 - -1.0 1,203,343 + -1.5 1,203,352 - +0.0 1,203,352 - -0.7
Or see this region's nucleotide sequence