Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lplT and LU632_RS06080 are separated by 90 nucleotides LU632_RS06080 and LU632_RS06085 are separated by 300 nucleotides LU632_RS06085 and LU632_RS06090 are separated by 167 nucleotides
LU632_RS06075: lplT - lysophospholipid transporter LplT, at 1,157,802 to 1,159,013
lplT
LU632_RS06080: LU632_RS06080 - NADP(H)-dependent aldo-keto reductase, at 1,159,104 to 1,160,144
_RS06080
LU632_RS06085: LU632_RS06085 - YgdI/YgdR family lipoprotein, at 1,160,445 to 1,160,660
_RS06085
LU632_RS06090: LU632_RS06090 - TerC family protein, at 1,160,828 to 1,161,538
_RS06090
Position (kb)
1159
1160
1161 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1158.115 kb on - strand, within lplT at 1158.178 kb on + strand, within lplT at 1158.179 kb on - strand, within lplT at 1158.318 kb on + strand, within lplT at 1158.319 kb on - strand, within lplT at 1158.319 kb on - strand, within lplT at 1158.319 kb on - strand, within lplT at 1158.319 kb on - strand, within lplT at 1158.554 kb on + strand, within lplT at 1158.708 kb on - strand, within lplT at 1158.708 kb on - strand, within lplT at 1158.708 kb on - strand, within lplT at 1158.788 kb on - strand, within lplT at 1158.883 kb on - strand, within lplT at 1158.883 kb on - strand, within lplT at 1158.892 kb on + strand at 1158.892 kb on + strand at 1158.895 kb on + strand at 1158.896 kb on - strand at 1158.896 kb on - strand at 1158.896 kb on - strand at 1159.050 kb on + strand at 1159.085 kb on - strand at 1159.121 kb on + strand at 1159.121 kb on + strand at 1159.122 kb on - strand at 1159.122 kb on - strand at 1159.134 kb on + strand at 1159.134 kb on + strand at 1159.134 kb on + strand at 1159.135 kb on - strand at 1159.135 kb on - strand at 1159.180 kb on + strand at 1159.180 kb on + strand at 1159.181 kb on - strand at 1159.181 kb on - strand at 1159.190 kb on + strand at 1159.376 kb on + strand, within LU632_RS06080 at 1159.379 kb on + strand, within LU632_RS06080 at 1159.379 kb on + strand, within LU632_RS06080 at 1159.380 kb on - strand, within LU632_RS06080 at 1159.380 kb on - strand, within LU632_RS06080 at 1159.380 kb on - strand, within LU632_RS06080 at 1159.380 kb on - strand, within LU632_RS06080 at 1159.411 kb on + strand, within LU632_RS06080 at 1159.412 kb on - strand, within LU632_RS06080 at 1159.447 kb on + strand, within LU632_RS06080 at 1159.448 kb on - strand, within LU632_RS06080 at 1159.448 kb on - strand, within LU632_RS06080 at 1159.620 kb on - strand, within LU632_RS06080 at 1159.620 kb on - strand, within LU632_RS06080 at 1159.622 kb on - strand, within LU632_RS06080 at 1159.709 kb on - strand, within LU632_RS06080 at 1159.782 kb on + strand, within LU632_RS06080 at 1159.942 kb on + strand, within LU632_RS06080 at 1160.130 kb on - strand at 1160.221 kb on - strand at 1160.230 kb on + strand at 1160.354 kb on + strand at 1160.421 kb on - strand at 1160.459 kb on + strand at 1160.459 kb on + strand at 1160.459 kb on + strand at 1160.460 kb on - strand at 1160.460 kb on - strand at 1160.460 kb on - strand at 1160.594 kb on - strand, within LU632_RS06085 at 1160.603 kb on + strand, within LU632_RS06085 at 1160.603 kb on + strand, within LU632_RS06085 at 1160.622 kb on - strand, within LU632_RS06085 at 1160.696 kb on + strand at 1160.697 kb on - strand at 1160.706 kb on + strand at 1160.706 kb on + strand at 1160.707 kb on - strand at 1160.707 kb on - strand at 1160.707 kb on - strand at 1160.709 kb on - strand at 1160.742 kb on - strand at 1160.786 kb on - strand at 1160.903 kb on - strand, within LU632_RS06090 at 1160.903 kb on - strand, within LU632_RS06090 at 1160.946 kb on + strand, within LU632_RS06090 at 1160.947 kb on + strand, within LU632_RS06090 at 1161.073 kb on + strand, within LU632_RS06090 at 1161.074 kb on - strand, within LU632_RS06090 at 1161.074 kb on - strand, within LU632_RS06090
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 1,158,115 - lplT LU632_RS06075 0.26 +1.1 1,158,178 + lplT LU632_RS06075 0.31 -1.2 1,158,179 - lplT LU632_RS06075 0.31 +0.4 1,158,318 + lplT LU632_RS06075 0.43 -0.2 1,158,319 - lplT LU632_RS06075 0.43 +1.7 1,158,319 - lplT LU632_RS06075 0.43 +0.1 1,158,319 - lplT LU632_RS06075 0.43 +0.1 1,158,319 - lplT LU632_RS06075 0.43 +0.6 1,158,554 + lplT LU632_RS06075 0.62 +1.6 1,158,708 - lplT LU632_RS06075 0.75 +1.4 1,158,708 - lplT LU632_RS06075 0.75 +0.8 1,158,708 - lplT LU632_RS06075 0.75 +0.8 1,158,788 - lplT LU632_RS06075 0.81 +0.6 1,158,883 - lplT LU632_RS06075 0.89 +0.9 1,158,883 - lplT LU632_RS06075 0.89 +0.8 1,158,892 + +0.1 1,158,892 + +0.1 1,158,895 + +0.5 1,158,896 - +0.8 1,158,896 - +2.6 1,158,896 - +0.8 1,159,050 + -1.2 1,159,085 - +0.9 1,159,121 + -2.2 1,159,121 + -2.0 1,159,122 - -0.2 1,159,122 - +1.8 1,159,134 + +0.5 1,159,134 + -1.6 1,159,134 + -0.8 1,159,135 - +1.0 1,159,135 - -1.5 1,159,180 + -0.2 1,159,180 + -0.2 1,159,181 - -1.0 1,159,181 - -1.8 1,159,190 + -0.2 1,159,376 + LU632_RS06080 0.26 -3.4 1,159,379 + LU632_RS06080 0.26 +0.0 1,159,379 + LU632_RS06080 0.26 -3.3 1,159,380 - LU632_RS06080 0.27 -1.3 1,159,380 - LU632_RS06080 0.27 -1.8 1,159,380 - LU632_RS06080 0.27 -0.9 1,159,380 - LU632_RS06080 0.27 -0.8 1,159,411 + LU632_RS06080 0.29 -0.2 1,159,412 - LU632_RS06080 0.30 +0.1 1,159,447 + LU632_RS06080 0.33 -1.2 1,159,448 - LU632_RS06080 0.33 -2.6 1,159,448 - LU632_RS06080 0.33 +0.4 1,159,620 - LU632_RS06080 0.50 +1.8 1,159,620 - LU632_RS06080 0.50 -1.8 1,159,622 - LU632_RS06080 0.50 -0.2 1,159,709 - LU632_RS06080 0.58 -1.5 1,159,782 + LU632_RS06080 0.65 -1.5 1,159,942 + LU632_RS06080 0.80 -2.2 1,160,130 - -1.5 1,160,221 - +0.0 1,160,230 + -0.0 1,160,354 + -1.1 1,160,421 - +0.8 1,160,459 + +0.0 1,160,459 + +0.4 1,160,459 + -0.1 1,160,460 - +0.6 1,160,460 - -0.2 1,160,460 - +0.7 1,160,594 - LU632_RS06085 0.69 +0.3 1,160,603 + LU632_RS06085 0.73 -0.0 1,160,603 + LU632_RS06085 0.73 -0.2 1,160,622 - LU632_RS06085 0.82 -0.8 1,160,696 + -0.7 1,160,697 - +0.3 1,160,706 + -0.8 1,160,706 + +1.6 1,160,707 - +0.9 1,160,707 - +0.8 1,160,707 - +0.4 1,160,709 - +0.5 1,160,742 - +1.6 1,160,786 - +0.4 1,160,903 - LU632_RS06090 0.11 -0.2 1,160,903 - LU632_RS06090 0.11 -1.1 1,160,946 + LU632_RS06090 0.17 +0.0 1,160,947 + LU632_RS06090 0.17 +0.8 1,161,073 + LU632_RS06090 0.34 +0.4 1,161,074 - LU632_RS06090 0.35 +0.5 1,161,074 - LU632_RS06090 0.35 -0.1
Or see this region's nucleotide sequence