Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspE and ppdD overlap by 1 nucleotides ppdD and nadC are separated by 195 nucleotides nadC and ampD are separated by 150 nucleotides ampD and ampE are separated by 39 nucleotides
LU632_RS03030: gspE - type II secretion system protein GspE, at 619,058 to 620,461
gspE
LU632_RS03035: ppdD - prepilin peptidase-dependent pilin, at 620,461 to 620,913
ppdD
LU632_RS03040: nadC - carboxylating nicotinate-nucleotide diphosphorylase, at 621,109 to 621,996
nadC
LU632_RS03045: ampD - 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD, at 622,147 to 622,697
ampD
LU632_RS03050: ampE - beta-lactamase regulator AmpE, at 622,737 to 623,545
ampE
Position (kb) 621
622 Strain fitness (log2 ratio) -2
-1
0
1
2 at 620.134 kb on + strand, within gspE at 620.159 kb on - strand, within gspE at 620.159 kb on - strand, within gspE at 620.189 kb on + strand, within gspE at 620.218 kb on + strand, within gspE at 620.295 kb on + strand, within gspE at 620.305 kb on + strand, within gspE at 620.314 kb on + strand, within gspE at 620.315 kb on - strand, within gspE at 620.324 kb on - strand at 620.342 kb on + strand at 620.342 kb on + strand at 620.343 kb on - strand at 620.392 kb on + strand at 620.428 kb on + strand at 620.428 kb on + strand at 620.429 kb on - strand at 620.559 kb on + strand, within ppdD at 620.559 kb on + strand, within ppdD at 620.560 kb on - strand, within ppdD at 620.672 kb on + strand, within ppdD at 620.673 kb on - strand, within ppdD at 620.756 kb on - strand, within ppdD at 620.767 kb on - strand, within ppdD at 620.816 kb on + strand, within ppdD at 620.817 kb on - strand, within ppdD at 620.892 kb on + strand at 620.893 kb on - strand at 620.918 kb on + strand at 620.919 kb on - strand at 621.032 kb on - strand at 621.032 kb on - strand at 621.032 kb on - strand at 621.032 kb on - strand at 621.127 kb on + strand at 621.144 kb on + strand at 621.194 kb on + strand at 621.342 kb on + strand, within nadC at 621.343 kb on - strand, within nadC at 621.455 kb on + strand, within nadC at 621.506 kb on + strand, within nadC at 621.507 kb on - strand, within nadC at 621.541 kb on - strand, within nadC at 621.541 kb on - strand, within nadC at 621.587 kb on + strand, within nadC at 621.588 kb on - strand, within nadC at 621.588 kb on - strand, within nadC at 621.588 kb on - strand, within nadC at 621.588 kb on - strand, within nadC at 621.654 kb on + strand, within nadC at 621.817 kb on + strand, within nadC at 621.817 kb on + strand, within nadC at 621.850 kb on + strand, within nadC at 621.850 kb on + strand, within nadC at 621.850 kb on + strand, within nadC at 621.851 kb on - strand, within nadC at 621.930 kb on + strand at 621.952 kb on + strand at 621.960 kb on + strand at 622.146 kb on + strand at 622.248 kb on + strand, within ampD at 622.248 kb on + strand, within ampD at 622.248 kb on + strand, within ampD at 622.249 kb on - strand, within ampD at 622.251 kb on + strand, within ampD at 622.251 kb on + strand, within ampD at 622.251 kb on + strand, within ampD at 622.333 kb on + strand, within ampD at 622.334 kb on - strand, within ampD at 622.398 kb on + strand, within ampD at 622.405 kb on + strand, within ampD at 622.450 kb on - strand, within ampD at 622.451 kb on + strand, within ampD at 622.451 kb on + strand, within ampD at 622.452 kb on - strand, within ampD at 622.454 kb on - strand, within ampD at 622.471 kb on + strand, within ampD at 622.510 kb on + strand, within ampD at 622.511 kb on - strand, within ampD at 622.535 kb on + strand, within ampD at 622.535 kb on + strand, within ampD at 622.593 kb on + strand, within ampD at 622.593 kb on + strand, within ampD at 622.594 kb on - strand, within ampD at 622.594 kb on - strand, within ampD at 622.595 kb on + strand, within ampD at 622.595 kb on + strand, within ampD at 622.596 kb on - strand, within ampD at 622.596 kb on - strand, within ampD at 622.596 kb on - strand, within ampD at 622.596 kb on - strand, within ampD at 622.596 kb on - strand, within ampD at 622.596 kb on - strand, within ampD at 622.596 kb on - strand, within ampD at 622.598 kb on - strand, within ampD at 622.598 kb on - strand, within ampD at 622.613 kb on + strand, within ampD at 622.613 kb on + strand, within ampD at 622.616 kb on + strand, within ampD at 622.616 kb on + strand, within ampD at 622.617 kb on - strand, within ampD at 622.617 kb on - strand, within ampD at 622.617 kb on - strand, within ampD at 622.693 kb on + strand at 622.693 kb on + strand at 622.693 kb on + strand at 622.694 kb on - strand at 622.694 kb on - strand at 622.695 kb on + strand at 622.696 kb on - strand at 622.696 kb on - strand at 622.696 kb on - strand at 622.696 kb on - strand at 622.696 kb on - strand at 622.696 kb on - strand at 622.696 kb on - strand at 622.696 kb on - strand at 622.696 kb on - strand at 622.696 kb on - strand at 622.696 kb on - strand at 622.844 kb on + strand, within ampE at 622.953 kb on - strand, within ampE at 622.981 kb on - strand, within ampE at 622.989 kb on + strand, within ampE at 622.989 kb on + strand, within ampE at 622.989 kb on + strand, within ampE
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 620,134 + gspE LU632_RS03030 0.77 -0.1 620,159 - gspE LU632_RS03030 0.78 -0.2 620,159 - gspE LU632_RS03030 0.78 -0.9 620,189 + gspE LU632_RS03030 0.81 -0.8 620,218 + gspE LU632_RS03030 0.83 -0.5 620,295 + gspE LU632_RS03030 0.88 -0.2 620,305 + gspE LU632_RS03030 0.89 -0.1 620,314 + gspE LU632_RS03030 0.89 -0.1 620,315 - gspE LU632_RS03030 0.90 +0.2 620,324 - +0.3 620,342 + +0.5 620,342 + -0.2 620,343 - +1.8 620,392 + +0.8 620,428 + -1.2 620,428 + -0.2 620,429 - -0.2 620,559 + ppdD LU632_RS03035 0.22 +0.4 620,559 + ppdD LU632_RS03035 0.22 -1.1 620,560 - ppdD LU632_RS03035 0.22 -0.4 620,672 + ppdD LU632_RS03035 0.47 +0.1 620,673 - ppdD LU632_RS03035 0.47 +1.1 620,756 - ppdD LU632_RS03035 0.65 -0.2 620,767 - ppdD LU632_RS03035 0.68 +0.5 620,816 + ppdD LU632_RS03035 0.78 +2.4 620,817 - ppdD LU632_RS03035 0.79 -1.1 620,892 + +0.5 620,893 - +0.8 620,918 + +0.8 620,919 - -0.2 621,032 - -0.0 621,032 - -0.1 621,032 - +0.2 621,032 - -0.0 621,127 + -0.8 621,144 + +0.5 621,194 + -0.8 621,342 + nadC LU632_RS03040 0.26 +0.8 621,343 - nadC LU632_RS03040 0.26 -0.4 621,455 + nadC LU632_RS03040 0.39 +1.5 621,506 + nadC LU632_RS03040 0.45 -1.2 621,507 - nadC LU632_RS03040 0.45 +0.6 621,541 - nadC LU632_RS03040 0.49 +0.3 621,541 - nadC LU632_RS03040 0.49 +0.5 621,587 + nadC LU632_RS03040 0.54 +0.4 621,588 - nadC LU632_RS03040 0.54 -0.2 621,588 - nadC LU632_RS03040 0.54 -0.2 621,588 - nadC LU632_RS03040 0.54 +0.9 621,588 - nadC LU632_RS03040 0.54 +0.7 621,654 + nadC LU632_RS03040 0.61 -1.5 621,817 + nadC LU632_RS03040 0.80 +0.2 621,817 + nadC LU632_RS03040 0.80 -0.6 621,850 + nadC LU632_RS03040 0.83 -1.8 621,850 + nadC LU632_RS03040 0.83 +0.6 621,850 + nadC LU632_RS03040 0.83 +0.6 621,851 - nadC LU632_RS03040 0.84 -0.8 621,930 + +0.0 621,952 + -0.3 621,960 + +0.4 622,146 + +0.8 622,248 + ampD LU632_RS03045 0.18 -1.3 622,248 + ampD LU632_RS03045 0.18 +0.5 622,248 + ampD LU632_RS03045 0.18 -0.2 622,249 - ampD LU632_RS03045 0.19 +1.4 622,251 + ampD LU632_RS03045 0.19 -0.2 622,251 + ampD LU632_RS03045 0.19 -0.2 622,251 + ampD LU632_RS03045 0.19 +0.2 622,333 + ampD LU632_RS03045 0.34 +0.8 622,334 - ampD LU632_RS03045 0.34 -0.2 622,398 + ampD LU632_RS03045 0.46 -0.8 622,405 + ampD LU632_RS03045 0.47 -0.0 622,450 - ampD LU632_RS03045 0.55 -1.2 622,451 + ampD LU632_RS03045 0.55 +0.3 622,451 + ampD LU632_RS03045 0.55 -1.3 622,452 - ampD LU632_RS03045 0.55 +0.1 622,454 - ampD LU632_RS03045 0.56 +0.3 622,471 + ampD LU632_RS03045 0.59 +0.5 622,510 + ampD LU632_RS03045 0.66 +0.8 622,511 - ampD LU632_RS03045 0.66 -0.2 622,535 + ampD LU632_RS03045 0.70 -2.2 622,535 + ampD LU632_RS03045 0.70 -0.2 622,593 + ampD LU632_RS03045 0.81 -1.2 622,593 + ampD LU632_RS03045 0.81 -0.8 622,594 - ampD LU632_RS03045 0.81 +0.8 622,594 - ampD LU632_RS03045 0.81 -0.2 622,595 + ampD LU632_RS03045 0.81 +0.8 622,595 + ampD LU632_RS03045 0.81 -0.5 622,596 - ampD LU632_RS03045 0.81 -0.3 622,596 - ampD LU632_RS03045 0.81 -0.3 622,596 - ampD LU632_RS03045 0.81 +0.3 622,596 - ampD LU632_RS03045 0.81 +0.2 622,596 - ampD LU632_RS03045 0.81 -0.7 622,596 - ampD LU632_RS03045 0.81 +0.4 622,596 - ampD LU632_RS03045 0.81 -0.2 622,598 - ampD LU632_RS03045 0.82 +0.3 622,598 - ampD LU632_RS03045 0.82 -0.1 622,613 + ampD LU632_RS03045 0.85 +0.3 622,613 + ampD LU632_RS03045 0.85 +0.8 622,616 + ampD LU632_RS03045 0.85 -0.5 622,616 + ampD LU632_RS03045 0.85 +1.4 622,617 - ampD LU632_RS03045 0.85 +0.2 622,617 - ampD LU632_RS03045 0.85 +0.1 622,617 - ampD LU632_RS03045 0.85 -0.9 622,693 + -0.6 622,693 + -1.2 622,693 + +0.5 622,694 - -0.8 622,694 - -1.2 622,695 + +0.8 622,696 - +1.4 622,696 - +1.0 622,696 - +0.7 622,696 - +0.7 622,696 - +0.2 622,696 - +0.8 622,696 - -0.5 622,696 - -0.5 622,696 - +1.4 622,696 - -1.8 622,696 - +0.0 622,844 + ampE LU632_RS03050 0.13 -0.5 622,953 - ampE LU632_RS03050 0.27 +0.4 622,981 - ampE LU632_RS03050 0.30 -0.7 622,989 + ampE LU632_RS03050 0.31 +0.3 622,989 + ampE LU632_RS03050 0.31 -0.5 622,989 + ampE LU632_RS03050 0.31 -0.2
Or see this region's nucleotide sequence