Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt apaH and folA are separated by 369 nucleotides folA and carB are separated by 571 nucleotides
LU632_RS02740: apaH - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH, at 551,218 to 552,048
apaH
LU632_RS02745: folA - type 3 dihydrofolate reductase, at 552,418 to 552,897
folA
LU632_RS02750: carB - carbamoyl-phosphate synthase large subunit, at 553,469 to 556,693
carB
Position (kb)
552
553 Strain fitness (log2 ratio)
-1
0
1
2 at 552.050 kb on + strand at 552.065 kb on + strand at 552.065 kb on + strand at 552.065 kb on + strand at 552.065 kb on + strand at 552.066 kb on - strand at 552.088 kb on + strand at 552.088 kb on + strand at 552.088 kb on + strand at 552.088 kb on + strand at 552.089 kb on - strand at 552.181 kb on - strand at 552.215 kb on + strand at 552.216 kb on - strand at 552.221 kb on - strand at 552.336 kb on + strand at 552.340 kb on + strand at 552.346 kb on - strand at 552.384 kb on - strand at 552.961 kb on + strand at 552.962 kb on - strand at 552.979 kb on + strand at 552.980 kb on - strand at 552.980 kb on - strand at 552.984 kb on - strand at 553.011 kb on - strand at 553.028 kb on + strand at 553.029 kb on - strand at 553.134 kb on + strand at 553.135 kb on - strand at 553.138 kb on + strand at 553.147 kb on + strand at 553.225 kb on + strand at 553.225 kb on + strand at 553.226 kb on - strand at 553.257 kb on - strand at 553.282 kb on + strand at 553.283 kb on - strand at 553.357 kb on - strand at 553.403 kb on + strand at 553.419 kb on - strand at 553.460 kb on + strand at 553.460 kb on + strand at 553.460 kb on + strand at 553.461 kb on - strand at 553.540 kb on + strand at 553.540 kb on + strand at 553.541 kb on - strand at 553.541 kb on - strand at 553.541 kb on - strand at 553.541 kb on - strand at 553.585 kb on + strand at 553.585 kb on + strand at 553.585 kb on + strand at 553.585 kb on + strand at 553.585 kb on + strand at 553.588 kb on - strand at 553.652 kb on - strand at 553.657 kb on + strand at 553.657 kb on + strand at 553.657 kb on + strand at 553.657 kb on + strand at 553.657 kb on + strand at 553.658 kb on - strand at 553.658 kb on - strand at 553.659 kb on + strand at 553.659 kb on + strand at 553.659 kb on + strand at 553.666 kb on - strand at 553.712 kb on + strand at 553.713 kb on - strand at 553.834 kb on + strand, within carB at 553.879 kb on + strand, within carB at 553.882 kb on + strand, within carB at 553.883 kb on - strand, within carB at 553.883 kb on - strand, within carB
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 552,050 + +0.8 552,065 + -0.2 552,065 + +0.2 552,065 + +0.6 552,065 + +1.2 552,066 - +0.1 552,088 + +0.5 552,088 + -0.2 552,088 + -0.2 552,088 + -1.2 552,089 - +0.3 552,181 - -1.2 552,215 + +0.5 552,216 - -0.2 552,221 - +0.0 552,336 + +0.4 552,340 + -0.4 552,346 - -1.0 552,384 - +0.1 552,961 + +0.7 552,962 - -1.2 552,979 + +1.8 552,980 - -0.1 552,980 - +0.8 552,984 - +1.0 553,011 - -0.2 553,028 + -1.0 553,029 - -0.1 553,134 + -0.2 553,135 - +0.2 553,138 + +0.8 553,147 + -0.1 553,225 + -0.8 553,225 + +0.8 553,226 - +0.5 553,257 - +0.9 553,282 + +0.5 553,283 - -0.5 553,357 - +0.7 553,403 + -0.6 553,419 - +0.6 553,460 + +0.8 553,460 + -1.2 553,460 + +0.3 553,461 - +0.9 553,540 + +2.1 553,540 + -0.9 553,541 - +0.1 553,541 - +0.3 553,541 - +0.8 553,541 - -0.1 553,585 + +0.8 553,585 + -0.6 553,585 + -0.2 553,585 + -0.5 553,585 + +0.1 553,588 - +0.8 553,652 - +1.2 553,657 + -0.9 553,657 + +1.2 553,657 + -0.2 553,657 + +0.8 553,657 + -0.3 553,658 - -0.4 553,658 - -0.8 553,659 + -0.2 553,659 + +0.8 553,659 + -0.3 553,666 - -0.6 553,712 + -0.4 553,713 - -0.0 553,834 + carB LU632_RS02750 0.11 +2.6 553,879 + carB LU632_RS02750 0.13 -1.2 553,882 + carB LU632_RS02750 0.13 -0.8 553,883 - carB LU632_RS02750 0.13 -0.2 553,883 - carB LU632_RS02750 0.13 +0.3
Or see this region's nucleotide sequence