Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt deoC and LU632_RS02520 are separated by 15 nucleotides LU632_RS02520 and deoB are separated by 79 nucleotides deoB and deoD are separated by 39 nucleotides deoD and LU632_RS02535 are separated by 187 nucleotides
LU632_RS02515: deoC - deoxyribose-phosphate aldolase, at 499,390 to 500,169
deoC
LU632_RS02520: LU632_RS02520 - thymidine phosphorylase, at 500,185 to 501,027
_RS02520
LU632_RS02525: deoB - phosphopentomutase, at 501,107 to 502,330
deoB
LU632_RS02530: deoD - purine-nucleoside phosphorylase, at 502,370 to 503,089
deoD
LU632_RS02535: LU632_RS02535 - IS1 family transposase, at 503,277 to 503,974
_RS02535
Position (kb) 501
502
503 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 500.331 kb on + strand, within LU632_RS02520 at 500.331 kb on + strand, within LU632_RS02520 at 500.331 kb on + strand, within LU632_RS02520 at 500.332 kb on - strand, within LU632_RS02520 at 500.332 kb on - strand, within LU632_RS02520 at 500.403 kb on + strand, within LU632_RS02520 at 500.425 kb on + strand, within LU632_RS02520 at 500.426 kb on - strand, within LU632_RS02520 at 500.426 kb on - strand, within LU632_RS02520 at 500.429 kb on - strand, within LU632_RS02520 at 500.494 kb on + strand, within LU632_RS02520 at 500.494 kb on + strand, within LU632_RS02520 at 500.494 kb on + strand, within LU632_RS02520 at 500.494 kb on + strand, within LU632_RS02520 at 500.495 kb on - strand, within LU632_RS02520 at 500.495 kb on - strand, within LU632_RS02520 at 500.495 kb on - strand, within LU632_RS02520 at 500.511 kb on + strand, within LU632_RS02520 at 500.857 kb on + strand, within LU632_RS02520 at 500.857 kb on + strand, within LU632_RS02520 at 500.858 kb on - strand, within LU632_RS02520 at 500.959 kb on + strand at 500.959 kb on + strand at 501.051 kb on + strand at 501.117 kb on + strand at 501.117 kb on + strand at 501.205 kb on + strand at 501.206 kb on - strand at 501.247 kb on + strand, within deoB at 501.247 kb on + strand, within deoB at 501.248 kb on - strand, within deoB at 501.248 kb on - strand, within deoB at 501.368 kb on + strand, within deoB at 501.397 kb on + strand, within deoB at 501.453 kb on - strand, within deoB at 501.586 kb on + strand, within deoB at 501.586 kb on + strand, within deoB at 501.586 kb on + strand, within deoB at 501.586 kb on + strand, within deoB at 501.587 kb on - strand, within deoB at 501.587 kb on - strand, within deoB at 501.612 kb on - strand, within deoB at 501.612 kb on - strand, within deoB at 501.677 kb on + strand, within deoB at 501.722 kb on + strand, within deoB at 501.722 kb on + strand, within deoB at 501.723 kb on - strand, within deoB at 501.724 kb on + strand, within deoB at 501.724 kb on + strand, within deoB at 501.724 kb on + strand, within deoB at 501.725 kb on - strand, within deoB at 501.725 kb on - strand, within deoB at 501.826 kb on + strand, within deoB at 501.827 kb on - strand, within deoB at 501.897 kb on - strand, within deoB at 501.913 kb on + strand, within deoB at 501.914 kb on - strand, within deoB at 501.914 kb on - strand, within deoB at 501.914 kb on - strand, within deoB at 502.195 kb on + strand, within deoB at 502.196 kb on - strand, within deoB at 502.211 kb on + strand at 502.286 kb on + strand at 502.286 kb on + strand at 502.322 kb on - strand at 502.343 kb on - strand at 502.384 kb on + strand at 502.385 kb on - strand at 502.386 kb on + strand at 502.387 kb on - strand at 502.488 kb on + strand, within deoD at 502.526 kb on + strand, within deoD at 502.526 kb on + strand, within deoD at 502.526 kb on + strand, within deoD at 502.527 kb on - strand, within deoD at 502.527 kb on - strand, within deoD at 502.527 kb on - strand, within deoD at 502.528 kb on + strand, within deoD at 502.665 kb on + strand, within deoD at 502.665 kb on + strand, within deoD at 502.725 kb on + strand, within deoD at 502.774 kb on - strand, within deoD at 502.850 kb on + strand, within deoD at 502.850 kb on + strand, within deoD at 502.850 kb on + strand, within deoD at 502.851 kb on - strand, within deoD at 502.851 kb on - strand, within deoD at 502.851 kb on - strand, within deoD at 502.890 kb on - strand, within deoD at 502.972 kb on + strand, within deoD at 502.973 kb on - strand, within deoD at 503.146 kb on + strand at 503.150 kb on + strand at 503.150 kb on + strand at 503.151 kb on - strand at 503.151 kb on - strand at 503.151 kb on - strand at 503.151 kb on - strand at 503.151 kb on - strand at 503.212 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 500,331 + LU632_RS02520 0.17 +1.0 500,331 + LU632_RS02520 0.17 +0.4 500,331 + LU632_RS02520 0.17 -0.2 500,332 - LU632_RS02520 0.17 -2.9 500,332 - LU632_RS02520 0.17 -2.2 500,403 + LU632_RS02520 0.26 -0.2 500,425 + LU632_RS02520 0.28 -0.6 500,426 - LU632_RS02520 0.29 -1.6 500,426 - LU632_RS02520 0.29 -1.2 500,429 - LU632_RS02520 0.29 -2.5 500,494 + LU632_RS02520 0.37 -0.4 500,494 + LU632_RS02520 0.37 -0.4 500,494 + LU632_RS02520 0.37 +0.4 500,494 + LU632_RS02520 0.37 -0.2 500,495 - LU632_RS02520 0.37 -4.1 500,495 - LU632_RS02520 0.37 -2.2 500,495 - LU632_RS02520 0.37 -3.9 500,511 + LU632_RS02520 0.39 +0.2 500,857 + LU632_RS02520 0.80 +0.3 500,857 + LU632_RS02520 0.80 +1.2 500,858 - LU632_RS02520 0.80 -1.8 500,959 + +1.4 500,959 + +1.0 501,051 + +2.0 501,117 + -4.3 501,117 + -3.4 501,205 + -1.2 501,206 - -4.4 501,247 + deoB LU632_RS02525 0.11 -3.4 501,247 + deoB LU632_RS02525 0.11 -1.4 501,248 - deoB LU632_RS02525 0.12 -3.7 501,248 - deoB LU632_RS02525 0.12 -3.2 501,368 + deoB LU632_RS02525 0.21 -3.5 501,397 + deoB LU632_RS02525 0.24 -1.2 501,453 - deoB LU632_RS02525 0.28 -1.2 501,586 + deoB LU632_RS02525 0.39 -3.2 501,586 + deoB LU632_RS02525 0.39 -2.2 501,586 + deoB LU632_RS02525 0.39 -4.2 501,586 + deoB LU632_RS02525 0.39 -3.0 501,587 - deoB LU632_RS02525 0.39 -3.1 501,587 - deoB LU632_RS02525 0.39 -1.2 501,612 - deoB LU632_RS02525 0.41 -2.4 501,612 - deoB LU632_RS02525 0.41 -0.2 501,677 + deoB LU632_RS02525 0.47 -0.8 501,722 + deoB LU632_RS02525 0.50 -2.4 501,722 + deoB LU632_RS02525 0.50 -2.0 501,723 - deoB LU632_RS02525 0.50 -2.7 501,724 + deoB LU632_RS02525 0.50 -1.2 501,724 + deoB LU632_RS02525 0.50 -3.5 501,724 + deoB LU632_RS02525 0.50 -2.8 501,725 - deoB LU632_RS02525 0.50 -3.0 501,725 - deoB LU632_RS02525 0.50 -2.2 501,826 + deoB LU632_RS02525 0.59 -1.8 501,827 - deoB LU632_RS02525 0.59 -5.3 501,897 - deoB LU632_RS02525 0.65 -1.8 501,913 + deoB LU632_RS02525 0.66 -2.5 501,914 - deoB LU632_RS02525 0.66 -2.8 501,914 - deoB LU632_RS02525 0.66 -3.8 501,914 - deoB LU632_RS02525 0.66 -5.2 502,195 + deoB LU632_RS02525 0.89 -0.2 502,196 - deoB LU632_RS02525 0.89 -3.0 502,211 + -2.7 502,286 + -1.8 502,286 + -1.8 502,322 - -1.8 502,343 - -1.8 502,384 + -1.2 502,385 - -4.0 502,386 + -1.8 502,387 - -1.2 502,488 + deoD LU632_RS02530 0.16 -3.9 502,526 + deoD LU632_RS02530 0.22 -2.7 502,526 + deoD LU632_RS02530 0.22 +0.8 502,526 + deoD LU632_RS02530 0.22 -1.2 502,527 - deoD LU632_RS02530 0.22 -0.2 502,527 - deoD LU632_RS02530 0.22 -1.5 502,527 - deoD LU632_RS02530 0.22 -1.2 502,528 + deoD LU632_RS02530 0.22 -3.7 502,665 + deoD LU632_RS02530 0.41 -2.3 502,665 + deoD LU632_RS02530 0.41 -3.0 502,725 + deoD LU632_RS02530 0.49 -3.4 502,774 - deoD LU632_RS02530 0.56 -1.8 502,850 + deoD LU632_RS02530 0.67 -3.4 502,850 + deoD LU632_RS02530 0.67 -2.8 502,850 + deoD LU632_RS02530 0.67 -1.8 502,851 - deoD LU632_RS02530 0.67 -1.8 502,851 - deoD LU632_RS02530 0.67 -2.2 502,851 - deoD LU632_RS02530 0.67 -3.3 502,890 - deoD LU632_RS02530 0.72 -2.5 502,972 + deoD LU632_RS02530 0.84 -2.5 502,973 - deoD LU632_RS02530 0.84 -2.8 503,146 + +0.6 503,150 + -0.5 503,150 + +1.0 503,151 - -0.2 503,151 - +1.5 503,151 - -0.5 503,151 - +1.8 503,151 - +0.4 503,212 + -1.2
Or see this region's nucleotide sequence