Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fbp and mpl are separated by 168 nucleotides mpl and LU632_RS02140 are separated by 61 nucleotides LU632_RS02140 and pmbA are separated by 166 nucleotides
LU632_RS02130: fbp - class 1 fructose-bisphosphatase, at 422,536 to 423,534
fbp
LU632_RS02135: mpl - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 423,703 to 425,082
mpl
LU632_RS02140: LU632_RS02140 - ribosome-associated protein, at 425,144 to 425,683
_RS02140
LU632_RS02145: pmbA - metalloprotease PmbA, at 425,850 to 427,190
pmbA
Position (kb) 423
424
425
426 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 423.621 kb on - strand at 423.624 kb on + strand at 423.624 kb on + strand at 423.624 kb on + strand at 423.625 kb on - strand at 423.625 kb on - strand at 423.625 kb on - strand at 423.625 kb on - strand at 423.699 kb on - strand at 423.699 kb on - strand at 423.702 kb on + strand at 423.708 kb on + strand at 423.708 kb on + strand at 423.714 kb on + strand at 423.715 kb on - strand at 423.725 kb on + strand at 423.849 kb on + strand, within mpl at 423.849 kb on + strand, within mpl at 423.859 kb on + strand, within mpl at 423.907 kb on - strand, within mpl at 424.186 kb on + strand, within mpl at 424.187 kb on - strand, within mpl at 424.300 kb on - strand, within mpl at 424.322 kb on + strand, within mpl at 424.322 kb on + strand, within mpl at 424.322 kb on + strand, within mpl at 424.323 kb on - strand, within mpl at 424.323 kb on - strand, within mpl at 424.618 kb on - strand, within mpl at 424.674 kb on - strand, within mpl at 424.682 kb on - strand, within mpl at 424.725 kb on + strand, within mpl at 424.791 kb on + strand, within mpl at 425.109 kb on - strand at 425.283 kb on - strand, within LU632_RS02140 at 425.365 kb on + strand, within LU632_RS02140 at 425.445 kb on - strand, within LU632_RS02140 at 425.550 kb on - strand, within LU632_RS02140 at 425.730 kb on + strand at 425.757 kb on + strand at 425.775 kb on + strand at 425.775 kb on - strand at 425.833 kb on - strand at 425.906 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 423,621 - +0.3 423,624 + +0.5 423,624 + +0.4 423,624 + -0.7 423,625 - -0.6 423,625 - +0.7 423,625 - +0.0 423,625 - +1.2 423,699 - -0.2 423,699 - -1.8 423,702 + -2.8 423,708 + -0.2 423,708 + -1.8 423,714 + -1.2 423,715 - -1.2 423,725 + -1.2 423,849 + mpl LU632_RS02135 0.11 -1.2 423,849 + mpl LU632_RS02135 0.11 -1.8 423,859 + mpl LU632_RS02135 0.11 -1.2 423,907 - mpl LU632_RS02135 0.15 -2.5 424,186 + mpl LU632_RS02135 0.35 -3.6 424,187 - mpl LU632_RS02135 0.35 -1.2 424,300 - mpl LU632_RS02135 0.43 -1.2 424,322 + mpl LU632_RS02135 0.45 -0.2 424,322 + mpl LU632_RS02135 0.45 -1.6 424,322 + mpl LU632_RS02135 0.45 -1.2 424,323 - mpl LU632_RS02135 0.45 -0.2 424,323 - mpl LU632_RS02135 0.45 -0.2 424,618 - mpl LU632_RS02135 0.66 -2.2 424,674 - mpl LU632_RS02135 0.70 -3.0 424,682 - mpl LU632_RS02135 0.71 +0.2 424,725 + mpl LU632_RS02135 0.74 -2.2 424,791 + mpl LU632_RS02135 0.79 -2.5 425,109 - +0.8 425,283 - LU632_RS02140 0.26 -0.4 425,365 + LU632_RS02140 0.41 -0.2 425,445 - LU632_RS02140 0.56 +0.8 425,550 - LU632_RS02140 0.75 -0.2 425,730 + -0.6 425,757 + +0.7 425,775 + -0.8 425,775 - -0.9 425,833 - -0.4 425,906 - +1.8
Or see this region's nucleotide sequence