Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutL and miaA overlap by 8 nucleotides miaA and hfq are separated by 101 nucleotides hfq and hflX are separated by 198 nucleotides
LU632_RS02045: mutL - DNA mismatch repair endonuclease MutL, at 401,138 to 403,030
mutL
LU632_RS02050: miaA - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 403,023 to 403,973
miaA
LU632_RS02055: hfq - RNA chaperone Hfq, at 404,075 to 404,386
hfq
LU632_RS02060: hflX - GTPase HflX, at 404,585 to 405,865
hflX
Position (kb) 403
404 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 402.042 kb on + strand, within mutL at 402.042 kb on + strand, within mutL at 402.087 kb on + strand, within mutL at 402.088 kb on - strand, within mutL at 402.390 kb on + strand, within mutL at 402.465 kb on + strand, within mutL at 402.466 kb on - strand, within mutL at 402.518 kb on + strand, within mutL at 402.682 kb on - strand, within mutL at 402.707 kb on + strand, within mutL at 402.708 kb on - strand, within mutL at 402.708 kb on - strand, within mutL at 402.708 kb on - strand, within mutL at 402.874 kb on + strand at 402.988 kb on + strand at 403.257 kb on + strand, within miaA at 403.356 kb on + strand, within miaA at 403.356 kb on + strand, within miaA at 403.356 kb on + strand, within miaA at 403.357 kb on - strand, within miaA at 403.367 kb on - strand, within miaA at 403.418 kb on + strand, within miaA at 403.722 kb on + strand, within miaA at 404.017 kb on + strand at 404.507 kb on + strand at 404.513 kb on + strand at 404.747 kb on - strand, within hflX at 404.932 kb on + strand, within hflX
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 402,042 + mutL LU632_RS02045 0.48 -2.2 402,042 + mutL LU632_RS02045 0.48 -0.5 402,087 + mutL LU632_RS02045 0.50 +1.6 402,088 - mutL LU632_RS02045 0.50 -0.8 402,390 + mutL LU632_RS02045 0.66 -2.8 402,465 + mutL LU632_RS02045 0.70 +1.4 402,466 - mutL LU632_RS02045 0.70 +1.8 402,518 + mutL LU632_RS02045 0.73 +0.3 402,682 - mutL LU632_RS02045 0.82 -0.2 402,707 + mutL LU632_RS02045 0.83 -0.4 402,708 - mutL LU632_RS02045 0.83 -1.8 402,708 - mutL LU632_RS02045 0.83 -0.1 402,708 - mutL LU632_RS02045 0.83 +0.6 402,874 + -1.2 402,988 + +0.5 403,257 + miaA LU632_RS02050 0.25 -0.2 403,356 + miaA LU632_RS02050 0.35 -1.2 403,356 + miaA LU632_RS02050 0.35 -1.2 403,356 + miaA LU632_RS02050 0.35 -1.2 403,357 - miaA LU632_RS02050 0.35 -0.2 403,367 - miaA LU632_RS02050 0.36 -1.2 403,418 + miaA LU632_RS02050 0.42 -3.5 403,722 + miaA LU632_RS02050 0.74 -0.9 404,017 + +0.8 404,507 + -0.1 404,513 + -1.6 404,747 - hflX LU632_RS02060 0.13 -2.2 404,932 + hflX LU632_RS02060 0.27 -1.8
Or see this region's nucleotide sequence