Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frsA and crl are separated by 104 nucleotides crl and LU632_RS01175 are separated by 79 nucleotides LU632_RS01175 and LU632_RS01180 are separated by 48 nucleotides LU632_RS01180 and proB are separated by 321 nucleotides
LU632_RS01165: frsA - esterase FrsA, at 247,716 to 248,948
frsA
LU632_RS01170: crl - sigma factor-binding protein Crl, at 249,053 to 249,454
crl
LU632_RS01175: LU632_RS01175 - porin, at 249,534 to 249,732
_RS01175
LU632_RS01180: LU632_RS01180 - hypothetical protein, at 249,781 to 249,870
_RS01180
LU632_RS01185: proB - glutamate 5-kinase, at 250,192 to 251,295
proB
Position (kb) 249
250 Strain fitness (log2 ratio) -2
-1
0
1
2 at 248.788 kb on - strand, within frsA at 248.788 kb on - strand, within frsA at 248.887 kb on + strand at 248.942 kb on - strand at 249.033 kb on + strand at 249.089 kb on - strand at 249.183 kb on + strand, within crl at 249.257 kb on + strand, within crl at 249.258 kb on - strand, within crl at 249.263 kb on + strand, within crl at 249.263 kb on + strand, within crl at 249.264 kb on - strand, within crl at 249.267 kb on - strand, within crl at 249.279 kb on - strand, within crl at 249.355 kb on + strand, within crl at 249.355 kb on + strand, within crl at 249.356 kb on - strand, within crl at 249.356 kb on - strand, within crl at 249.401 kb on - strand, within crl at 249.509 kb on + strand at 249.510 kb on - strand at 249.519 kb on + strand at 249.519 kb on + strand at 249.519 kb on + strand at 249.519 kb on + strand at 249.520 kb on - strand at 249.520 kb on - strand at 249.520 kb on - strand at 249.520 kb on - strand at 249.528 kb on - strand at 249.652 kb on + strand, within LU632_RS01175 at 249.653 kb on - strand, within LU632_RS01175 at 249.656 kb on - strand, within LU632_RS01175 at 249.656 kb on - strand, within LU632_RS01175 at 249.656 kb on - strand, within LU632_RS01175 at 249.676 kb on + strand, within LU632_RS01175 at 249.676 kb on + strand, within LU632_RS01175 at 249.676 kb on + strand, within LU632_RS01175 at 249.677 kb on - strand, within LU632_RS01175 at 249.677 kb on - strand, within LU632_RS01175 at 249.677 kb on - strand, within LU632_RS01175 at 249.687 kb on - strand, within LU632_RS01175 at 249.731 kb on + strand at 249.785 kb on + strand at 249.785 kb on + strand at 249.810 kb on + strand, within LU632_RS01180 at 250.021 kb on - strand at 250.149 kb on + strand at 250.149 kb on + strand at 250.163 kb on + strand at 250.164 kb on - strand at 250.170 kb on - strand at 250.174 kb on - strand at 250.238 kb on + strand at 250.272 kb on + strand at 250.273 kb on - strand at 250.273 kb on - strand at 250.273 kb on - strand at 250.416 kb on - strand, within proB at 250.472 kb on - strand, within proB at 250.482 kb on + strand, within proB at 250.482 kb on + strand, within proB at 250.484 kb on + strand, within proB at 250.484 kb on + strand, within proB at 250.485 kb on - strand, within proB at 250.485 kb on - strand, within proB at 250.553 kb on + strand, within proB at 250.553 kb on + strand, within proB at 250.553 kb on + strand, within proB at 250.554 kb on - strand, within proB at 250.575 kb on + strand, within proB at 250.575 kb on + strand, within proB at 250.576 kb on - strand, within proB at 250.576 kb on - strand, within proB at 250.809 kb on - strand, within proB
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 248,788 - frsA LU632_RS01165 0.87 +0.4 248,788 - frsA LU632_RS01165 0.87 +0.4 248,887 + -1.2 248,942 - +0.2 249,033 + +0.8 249,089 - +0.9 249,183 + crl LU632_RS01170 0.32 +1.1 249,257 + crl LU632_RS01170 0.51 -0.0 249,258 - crl LU632_RS01170 0.51 +0.0 249,263 + crl LU632_RS01170 0.52 -0.2 249,263 + crl LU632_RS01170 0.52 -2.0 249,264 - crl LU632_RS01170 0.52 -1.2 249,267 - crl LU632_RS01170 0.53 +0.4 249,279 - crl LU632_RS01170 0.56 +0.2 249,355 + crl LU632_RS01170 0.75 -0.2 249,355 + crl LU632_RS01170 0.75 -1.8 249,356 - crl LU632_RS01170 0.75 +0.6 249,356 - crl LU632_RS01170 0.75 -0.4 249,401 - crl LU632_RS01170 0.87 +0.4 249,509 + -0.8 249,510 - -1.4 249,519 + +0.5 249,519 + +0.3 249,519 + +0.5 249,519 + +0.8 249,520 - +1.4 249,520 - +1.0 249,520 - -0.4 249,520 - +0.1 249,528 - +0.5 249,652 + LU632_RS01175 0.59 -1.2 249,653 - LU632_RS01175 0.60 +1.8 249,656 - LU632_RS01175 0.61 +0.8 249,656 - LU632_RS01175 0.61 -0.2 249,656 - LU632_RS01175 0.61 +0.4 249,676 + LU632_RS01175 0.71 -0.4 249,676 + LU632_RS01175 0.71 -0.6 249,676 + LU632_RS01175 0.71 -1.2 249,677 - LU632_RS01175 0.72 -0.2 249,677 - LU632_RS01175 0.72 -0.6 249,677 - LU632_RS01175 0.72 +0.2 249,687 - LU632_RS01175 0.77 +0.2 249,731 + -0.2 249,785 + -0.2 249,785 + -0.3 249,810 + LU632_RS01180 0.32 -0.9 250,021 - +0.4 250,149 + +0.0 250,149 + +0.9 250,163 + -0.1 250,164 - +1.2 250,170 - +1.4 250,174 - -0.5 250,238 + +0.6 250,272 + -1.2 250,273 - +0.2 250,273 - +1.0 250,273 - -0.4 250,416 - proB LU632_RS01185 0.20 -0.3 250,472 - proB LU632_RS01185 0.25 +2.6 250,482 + proB LU632_RS01185 0.26 -0.2 250,482 + proB LU632_RS01185 0.26 -0.2 250,484 + proB LU632_RS01185 0.26 -0.3 250,484 + proB LU632_RS01185 0.26 +1.8 250,485 - proB LU632_RS01185 0.27 +0.2 250,485 - proB LU632_RS01185 0.27 +0.8 250,553 + proB LU632_RS01185 0.33 -0.3 250,553 + proB LU632_RS01185 0.33 -1.0 250,553 + proB LU632_RS01185 0.33 -0.4 250,554 - proB LU632_RS01185 0.33 -0.4 250,575 + proB LU632_RS01185 0.35 -0.2 250,575 + proB LU632_RS01185 0.35 -0.7 250,576 - proB LU632_RS01185 0.35 -1.2 250,576 - proB LU632_RS01185 0.35 +0.5 250,809 - proB LU632_RS01185 0.56 +0.2
Or see this region's nucleotide sequence