Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnpA and LU632_RS01070 are separated by 559 nucleotides LU632_RS01070 and mltD are separated by 359 nucleotides
LU632_RS01065: tnpA - IS200/IS605 family transposase, at 229,452 to 229,888
tnpA
LU632_RS01070: LU632_RS01070 - endonuclease/exonuclease/phosphatase family protein, at 230,448 to 231,254
_RS01070
LU632_RS01075: mltD - murein transglycosylase D, at 231,614 to 233,002
mltD
Position (kb)
230
231
232 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 230.067 kb on + strand at 230.067 kb on + strand at 230.135 kb on + strand at 230.136 kb on - strand at 230.149 kb on + strand at 230.150 kb on - strand at 230.153 kb on + strand at 230.154 kb on - strand at 230.154 kb on - strand at 230.155 kb on + strand at 230.155 kb on + strand at 230.156 kb on - strand at 230.160 kb on - strand at 230.160 kb on - strand at 230.199 kb on + strand at 230.199 kb on + strand at 230.273 kb on - strand at 230.275 kb on + strand at 230.301 kb on + strand at 230.301 kb on + strand at 230.302 kb on - strand at 230.302 kb on - strand at 230.317 kb on + strand at 230.338 kb on + strand at 230.339 kb on - strand at 230.361 kb on - strand at 230.384 kb on + strand at 230.432 kb on - strand at 230.475 kb on + strand at 230.476 kb on - strand at 230.479 kb on + strand at 230.480 kb on - strand at 230.501 kb on + strand at 230.519 kb on + strand at 230.519 kb on + strand at 230.519 kb on + strand at 230.665 kb on + strand, within LU632_RS01070 at 230.665 kb on + strand, within LU632_RS01070 at 230.666 kb on - strand, within LU632_RS01070 at 230.666 kb on - strand, within LU632_RS01070 at 230.666 kb on - strand, within LU632_RS01070 at 230.752 kb on + strand, within LU632_RS01070 at 230.805 kb on + strand, within LU632_RS01070 at 230.910 kb on - strand, within LU632_RS01070 at 230.927 kb on + strand, within LU632_RS01070 at 230.945 kb on - strand, within LU632_RS01070 at 230.945 kb on - strand, within LU632_RS01070 at 230.946 kb on + strand, within LU632_RS01070 at 230.946 kb on + strand, within LU632_RS01070 at 230.981 kb on + strand, within LU632_RS01070 at 230.981 kb on + strand, within LU632_RS01070 at 231.053 kb on - strand, within LU632_RS01070 at 231.068 kb on + strand, within LU632_RS01070 at 231.072 kb on - strand, within LU632_RS01070 at 231.089 kb on - strand, within LU632_RS01070 at 231.225 kb on - strand at 231.249 kb on - strand at 231.255 kb on + strand at 231.255 kb on + strand at 231.255 kb on + strand at 231.255 kb on + strand at 231.256 kb on - strand at 231.256 kb on - strand at 231.265 kb on - strand at 231.265 kb on - strand at 231.351 kb on + strand at 231.370 kb on + strand at 231.402 kb on - strand at 231.402 kb on - strand at 231.404 kb on + strand at 231.481 kb on + strand at 231.482 kb on - strand at 231.484 kb on - strand at 231.494 kb on + strand at 231.514 kb on + strand at 231.514 kb on + strand at 231.672 kb on - strand at 231.770 kb on - strand, within mltD at 231.770 kb on - strand, within mltD at 231.770 kb on - strand, within mltD at 231.770 kb on - strand, within mltD at 231.770 kb on - strand, within mltD at 231.770 kb on - strand, within mltD at 231.770 kb on - strand, within mltD at 231.771 kb on + strand, within mltD at 231.771 kb on + strand, within mltD at 231.771 kb on + strand, within mltD at 231.771 kb on + strand, within mltD at 231.772 kb on - strand, within mltD at 231.776 kb on - strand, within mltD at 231.776 kb on - strand, within mltD at 231.776 kb on - strand, within mltD at 231.777 kb on + strand, within mltD at 231.777 kb on + strand, within mltD at 231.777 kb on + strand, within mltD at 231.777 kb on + strand, within mltD at 231.777 kb on + strand, within mltD at 231.778 kb on - strand, within mltD at 231.778 kb on - strand, within mltD at 231.778 kb on - strand, within mltD at 231.778 kb on - strand, within mltD at 231.778 kb on - strand, within mltD at 231.954 kb on + strand, within mltD at 231.955 kb on - strand, within mltD at 231.955 kb on - strand, within mltD at 231.977 kb on - strand, within mltD at 231.977 kb on - strand, within mltD at 232.049 kb on - strand, within mltD at 232.064 kb on - strand, within mltD at 232.064 kb on - strand, within mltD at 232.069 kb on - strand, within mltD at 232.069 kb on - strand, within mltD at 232.252 kb on + strand, within mltD
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 230,067 + -0.2 230,067 + -2.5 230,135 + +0.4 230,136 - -0.8 230,149 + -1.5 230,150 - -1.3 230,153 + +1.1 230,154 - +0.4 230,154 - -1.8 230,155 + -0.8 230,155 + -0.3 230,156 - -1.0 230,160 - -0.6 230,160 - +0.4 230,199 + +0.9 230,199 + +0.8 230,273 - +0.7 230,275 + -0.1 230,301 + +1.4 230,301 + +0.1 230,302 - +0.5 230,302 - +0.9 230,317 + +0.6 230,338 + -0.2 230,339 - -1.8 230,361 - +2.2 230,384 + +0.2 230,432 - -1.8 230,475 + -2.9 230,476 - -2.8 230,479 + -0.7 230,480 - -0.8 230,501 + -2.1 230,519 + -1.4 230,519 + -1.3 230,519 + -2.0 230,665 + LU632_RS01070 0.27 -2.4 230,665 + LU632_RS01070 0.27 -1.1 230,666 - LU632_RS01070 0.27 -0.9 230,666 - LU632_RS01070 0.27 -1.8 230,666 - LU632_RS01070 0.27 -0.9 230,752 + LU632_RS01070 0.38 -0.8 230,805 + LU632_RS01070 0.44 -1.9 230,910 - LU632_RS01070 0.57 -0.9 230,927 + LU632_RS01070 0.59 -2.2 230,945 - LU632_RS01070 0.62 -0.8 230,945 - LU632_RS01070 0.62 -2.4 230,946 + LU632_RS01070 0.62 -1.8 230,946 + LU632_RS01070 0.62 -0.2 230,981 + LU632_RS01070 0.66 -1.2 230,981 + LU632_RS01070 0.66 -2.6 231,053 - LU632_RS01070 0.75 -1.7 231,068 + LU632_RS01070 0.77 -2.0 231,072 - LU632_RS01070 0.77 -2.2 231,089 - LU632_RS01070 0.79 -0.2 231,225 - -0.8 231,249 - -2.0 231,255 + +0.4 231,255 + -0.3 231,255 + +0.5 231,255 + +0.8 231,256 - +2.3 231,256 - -0.4 231,265 - -0.7 231,265 - -0.2 231,351 + +0.4 231,370 + +0.4 231,402 - +0.2 231,402 - +1.1 231,404 + +0.4 231,481 + +0.1 231,482 - +0.0 231,484 - -0.0 231,494 + -0.3 231,514 + +0.3 231,514 + -0.2 231,672 - -0.2 231,770 - mltD LU632_RS01075 0.11 +2.0 231,770 - mltD LU632_RS01075 0.11 +0.8 231,770 - mltD LU632_RS01075 0.11 -0.1 231,770 - mltD LU632_RS01075 0.11 +0.6 231,770 - mltD LU632_RS01075 0.11 -1.2 231,770 - mltD LU632_RS01075 0.11 -1.2 231,770 - mltD LU632_RS01075 0.11 -2.2 231,771 + mltD LU632_RS01075 0.11 +0.1 231,771 + mltD LU632_RS01075 0.11 +0.3 231,771 + mltD LU632_RS01075 0.11 +0.5 231,771 + mltD LU632_RS01075 0.11 +0.2 231,772 - mltD LU632_RS01075 0.11 +0.8 231,776 - mltD LU632_RS01075 0.12 +0.5 231,776 - mltD LU632_RS01075 0.12 -0.7 231,776 - mltD LU632_RS01075 0.12 -0.6 231,777 + mltD LU632_RS01075 0.12 +0.5 231,777 + mltD LU632_RS01075 0.12 -1.6 231,777 + mltD LU632_RS01075 0.12 -0.2 231,777 + mltD LU632_RS01075 0.12 -0.4 231,777 + mltD LU632_RS01075 0.12 -0.1 231,778 - mltD LU632_RS01075 0.12 -0.2 231,778 - mltD LU632_RS01075 0.12 +0.7 231,778 - mltD LU632_RS01075 0.12 -0.2 231,778 - mltD LU632_RS01075 0.12 +0.2 231,778 - mltD LU632_RS01075 0.12 +1.1 231,954 + mltD LU632_RS01075 0.24 +1.8 231,955 - mltD LU632_RS01075 0.25 +0.0 231,955 - mltD LU632_RS01075 0.25 +0.3 231,977 - mltD LU632_RS01075 0.26 +0.3 231,977 - mltD LU632_RS01075 0.26 +0.2 232,049 - mltD LU632_RS01075 0.31 -0.2 232,064 - mltD LU632_RS01075 0.32 -0.2 232,064 - mltD LU632_RS01075 0.32 -1.4 232,069 - mltD LU632_RS01075 0.33 +0.6 232,069 - mltD LU632_RS01075 0.33 -0.5 232,252 + mltD LU632_RS01075 0.46 -1.6
Or see this region's nucleotide sequence