Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aroE and tsaC are separated by 5 nucleotides tsaC and LU632_RS22455 overlap by 8 nucleotides LU632_RS22455 and smg are separated by 27 nucleotides smg and dprA overlap by 29 nucleotides
LU632_RS22445: aroE - shikimate dehydrogenase, at 4,290,060 to 4,290,881
aroE
LU632_RS22450: tsaC - L-threonylcarbamoyladenylate synthase type 1 TsaC, at 4,290,887 to 4,291,459
tsaC
LU632_RS22455: LU632_RS22455 - topoisomerase DNA-binding C4 zinc finger domain-containing protein, at 4,291,452 to 4,292,006
_RS22455
LU632_RS22460: smg - DUF494 family protein Smg, at 4,292,034 to 4,292,507
smg
LU632_RS22465: dprA - DNA-protecting protein DprA, at 4,292,479 to 4,293,603
dprA
Position (kb) 4291
4292
4293 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4290.475 kb on - strand, within aroE at 4290.620 kb on + strand, within aroE at 4290.686 kb on + strand, within aroE at 4290.728 kb on + strand, within aroE at 4290.822 kb on + strand at 4290.822 kb on + strand at 4290.823 kb on - strand at 4290.823 kb on - strand at 4290.823 kb on - strand at 4290.825 kb on - strand at 4291.755 kb on - strand, within LU632_RS22455 at 4291.755 kb on - strand, within LU632_RS22455 at 4291.820 kb on - strand, within LU632_RS22455 at 4291.880 kb on - strand, within LU632_RS22455 at 4292.006 kb on + strand at 4292.008 kb on + strand at 4292.008 kb on + strand at 4292.009 kb on - strand at 4292.009 kb on - strand at 4292.013 kb on + strand at 4292.013 kb on + strand at 4292.014 kb on - strand at 4292.014 kb on - strand at 4292.140 kb on - strand, within smg at 4292.265 kb on + strand, within smg at 4292.265 kb on + strand, within smg at 4292.266 kb on - strand, within smg at 4292.266 kb on - strand, within smg at 4292.291 kb on - strand, within smg at 4292.294 kb on + strand, within smg at 4292.295 kb on - strand, within smg at 4292.387 kb on - strand, within smg at 4292.430 kb on + strand, within smg at 4292.435 kb on + strand, within smg at 4292.473 kb on + strand at 4292.474 kb on - strand at 4292.474 kb on - strand at 4292.488 kb on + strand at 4292.488 kb on + strand at 4292.488 kb on + strand at 4292.489 kb on - strand at 4292.489 kb on - strand at 4292.489 kb on - strand at 4292.493 kb on + strand at 4292.494 kb on - strand at 4292.532 kb on - strand at 4292.688 kb on + strand, within dprA at 4292.689 kb on - strand, within dprA at 4292.708 kb on + strand, within dprA at 4292.708 kb on + strand, within dprA at 4292.709 kb on - strand, within dprA at 4292.709 kb on - strand, within dprA at 4292.710 kb on + strand, within dprA at 4292.772 kb on + strand, within dprA at 4292.773 kb on - strand, within dprA at 4292.821 kb on - strand, within dprA at 4292.930 kb on - strand, within dprA at 4292.930 kb on - strand, within dprA at 4292.930 kb on - strand, within dprA at 4292.962 kb on + strand, within dprA
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 4,290,475 - aroE LU632_RS22445 0.50 -1.0 4,290,620 + aroE LU632_RS22445 0.68 -1.0 4,290,686 + aroE LU632_RS22445 0.76 -3.1 4,290,728 + aroE LU632_RS22445 0.81 -1.6 4,290,822 + -1.0 4,290,822 + +0.0 4,290,823 - -1.0 4,290,823 - -2.6 4,290,823 - -2.0 4,290,825 - +0.0 4,291,755 - LU632_RS22455 0.55 +1.6 4,291,755 - LU632_RS22455 0.55 -1.0 4,291,820 - LU632_RS22455 0.66 -0.6 4,291,880 - LU632_RS22455 0.77 -1.2 4,292,006 + -1.6 4,292,008 + -1.6 4,292,008 + -0.1 4,292,009 - -0.1 4,292,009 - +0.0 4,292,013 + -0.4 4,292,013 + -3.1 4,292,014 - +1.1 4,292,014 - +0.5 4,292,140 - smg LU632_RS22460 0.22 -0.6 4,292,265 + smg LU632_RS22460 0.49 +1.0 4,292,265 + smg LU632_RS22460 0.49 -1.0 4,292,266 - smg LU632_RS22460 0.49 +1.6 4,292,266 - smg LU632_RS22460 0.49 +0.1 4,292,291 - smg LU632_RS22460 0.54 -1.1 4,292,294 + smg LU632_RS22460 0.55 -1.0 4,292,295 - smg LU632_RS22460 0.55 +0.3 4,292,387 - smg LU632_RS22460 0.74 -1.0 4,292,430 + smg LU632_RS22460 0.84 +1.0 4,292,435 + smg LU632_RS22460 0.85 +2.2 4,292,473 + -1.0 4,292,474 - -2.0 4,292,474 - -0.6 4,292,488 + -1.6 4,292,488 + -0.6 4,292,488 + -1.7 4,292,489 - -0.9 4,292,489 - +0.6 4,292,489 - -0.5 4,292,493 + -0.3 4,292,494 - +0.6 4,292,532 - +0.6 4,292,688 + dprA LU632_RS22465 0.19 -1.4 4,292,689 - dprA LU632_RS22465 0.19 +2.0 4,292,708 + dprA LU632_RS22465 0.20 -0.3 4,292,708 + dprA LU632_RS22465 0.20 -0.6 4,292,709 - dprA LU632_RS22465 0.20 -1.6 4,292,709 - dprA LU632_RS22465 0.20 +0.4 4,292,710 + dprA LU632_RS22465 0.21 -0.2 4,292,772 + dprA LU632_RS22465 0.26 +0.0 4,292,773 - dprA LU632_RS22465 0.26 -2.0 4,292,821 - dprA LU632_RS22465 0.30 +0.2 4,292,930 - dprA LU632_RS22465 0.40 -1.0 4,292,930 - dprA LU632_RS22465 0.40 +0.3 4,292,930 - dprA LU632_RS22465 0.40 -0.3 4,292,962 + dprA LU632_RS22465 0.43 -0.0
Or see this region's nucleotide sequence