Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proS and arfB are separated by 389 nucleotides arfB and LU632_RS19855 overlap by 4 nucleotides LU632_RS19855 and LU632_RS19860 are separated by 129 nucleotides LU632_RS19860 and rof overlap by 14 nucleotides
LU632_RS19845: proS - proline--tRNA ligase, at 3,770,882 to 3,772,600
proS
LU632_RS19850: arfB - aminoacyl-tRNA hydrolase, at 3,772,990 to 3,773,409
arfB
LU632_RS19855: LU632_RS19855 - YaeQ family protein, at 3,773,406 to 3,773,960
_RS19855
LU632_RS19860: LU632_RS19860 - YaeP family protein, at 3,774,090 to 3,774,290
_RS19860
LU632_RS19865: rof - Rho-binding antiterminator, at 3,774,277 to 3,774,540
rof
Position (kb)
3772
3773
3774 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3772.604 kb on + strand at 3772.605 kb on - strand at 3772.635 kb on + strand at 3772.636 kb on - strand at 3772.678 kb on + strand at 3772.706 kb on + strand at 3772.707 kb on - strand at 3772.816 kb on + strand at 3772.816 kb on + strand at 3772.816 kb on + strand at 3772.817 kb on - strand at 3772.817 kb on - strand at 3772.827 kb on - strand at 3773.197 kb on - strand, within arfB at 3773.316 kb on + strand, within arfB at 3773.320 kb on - strand, within arfB at 3773.445 kb on + strand at 3773.459 kb on + strand at 3773.597 kb on + strand, within LU632_RS19855 at 3773.598 kb on - strand, within LU632_RS19855 at 3773.598 kb on - strand, within LU632_RS19855 at 3773.640 kb on - strand, within LU632_RS19855 at 3773.641 kb on + strand, within LU632_RS19855 at 3773.647 kb on + strand, within LU632_RS19855 at 3773.647 kb on + strand, within LU632_RS19855 at 3773.647 kb on + strand, within LU632_RS19855 at 3773.648 kb on - strand, within LU632_RS19855 at 3773.648 kb on - strand, within LU632_RS19855 at 3773.648 kb on - strand, within LU632_RS19855 at 3773.648 kb on - strand, within LU632_RS19855 at 3773.649 kb on + strand, within LU632_RS19855 at 3773.649 kb on + strand, within LU632_RS19855 at 3773.649 kb on + strand, within LU632_RS19855 at 3773.650 kb on - strand, within LU632_RS19855 at 3773.650 kb on - strand, within LU632_RS19855 at 3773.793 kb on + strand, within LU632_RS19855 at 3773.793 kb on + strand, within LU632_RS19855 at 3773.794 kb on - strand, within LU632_RS19855 at 3773.925 kb on - strand at 3773.936 kb on + strand at 3773.937 kb on - strand at 3773.937 kb on - strand at 3773.937 kb on - strand at 3773.937 kb on - strand at 3773.938 kb on + strand at 3773.939 kb on - strand at 3773.940 kb on + strand at 3773.941 kb on - strand at 3773.973 kb on - strand at 3773.986 kb on + strand at 3774.053 kb on - strand at 3774.153 kb on + strand, within LU632_RS19860 at 3774.217 kb on + strand, within LU632_RS19860 at 3774.217 kb on + strand, within LU632_RS19860 at 3774.218 kb on - strand, within LU632_RS19860 at 3774.218 kb on - strand, within LU632_RS19860 at 3774.228 kb on - strand, within LU632_RS19860 at 3774.276 kb on + strand at 3774.276 kb on + strand at 3774.277 kb on - strand at 3774.277 kb on - strand at 3774.319 kb on + strand, within rof
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 3,772,604 + +1.0 3,772,605 - +1.0 3,772,635 + -0.1 3,772,636 - +1.5 3,772,678 + +1.0 3,772,706 + -0.6 3,772,707 - +0.4 3,772,816 + -1.6 3,772,816 + -0.3 3,772,816 + -0.1 3,772,817 - +0.6 3,772,817 - +0.6 3,772,827 - +0.6 3,773,197 - arfB LU632_RS19850 0.49 +1.8 3,773,316 + arfB LU632_RS19850 0.78 -0.1 3,773,320 - arfB LU632_RS19850 0.79 +1.4 3,773,445 + +1.0 3,773,459 + +1.0 3,773,597 + LU632_RS19855 0.34 -0.4 3,773,598 - LU632_RS19855 0.35 -0.2 3,773,598 - LU632_RS19855 0.35 -0.5 3,773,640 - LU632_RS19855 0.42 -0.4 3,773,641 + LU632_RS19855 0.42 -0.6 3,773,647 + LU632_RS19855 0.43 +0.3 3,773,647 + LU632_RS19855 0.43 +0.3 3,773,647 + LU632_RS19855 0.43 +0.0 3,773,648 - LU632_RS19855 0.44 -0.5 3,773,648 - LU632_RS19855 0.44 +1.0 3,773,648 - LU632_RS19855 0.44 -1.0 3,773,648 - LU632_RS19855 0.44 +0.3 3,773,649 + LU632_RS19855 0.44 +0.4 3,773,649 + LU632_RS19855 0.44 +0.1 3,773,649 + LU632_RS19855 0.44 +0.5 3,773,650 - LU632_RS19855 0.44 -0.6 3,773,650 - LU632_RS19855 0.44 -0.5 3,773,793 + LU632_RS19855 0.70 +1.0 3,773,793 + LU632_RS19855 0.70 +0.6 3,773,794 - LU632_RS19855 0.70 -0.6 3,773,925 - +0.3 3,773,936 + -0.6 3,773,937 - +0.0 3,773,937 - +0.6 3,773,937 - +0.1 3,773,937 - -0.2 3,773,938 + -0.3 3,773,939 - +1.2 3,773,940 + +1.1 3,773,941 - +0.7 3,773,973 - +0.6 3,773,986 + -0.2 3,774,053 - +0.7 3,774,153 + LU632_RS19860 0.31 -1.0 3,774,217 + LU632_RS19860 0.63 -0.6 3,774,217 + LU632_RS19860 0.63 -3.7 3,774,218 - LU632_RS19860 0.64 -2.0 3,774,218 - LU632_RS19860 0.64 -0.9 3,774,228 - LU632_RS19860 0.69 -2.0 3,774,276 + -1.0 3,774,276 + +1.0 3,774,277 - +0.0 3,774,277 - -0.6 3,774,319 + rof LU632_RS19865 0.16 +0.0
Or see this region's nucleotide sequence