Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS16655 and LU632_RS16660 are separated by 60 nucleotides LU632_RS16660 and LU632_RS16665 overlap by 4 nucleotides LU632_RS16665 and LU632_RS16670 are separated by 84 nucleotides LU632_RS16670 and LU632_RS16675 are separated by 68 nucleotides LU632_RS16675 and LU632_RS16680 overlap by 4 nucleotides LU632_RS16680 and LU632_RS16685 overlap by 19 nucleotides
LU632_RS16655: LU632_RS16655 - IS3 family transposase, at 3,146,376 to 3,147,496
_RS16655
LU632_RS16660: LU632_RS16660 - DNA-binding protein, at 3,147,557 to 3,148,347
_RS16660
LU632_RS16665: LU632_RS16665 - hypothetical protein, at 3,148,344 to 3,148,577
_RS16665
LU632_RS16670: LU632_RS16670 - hypothetical protein, at 3,148,662 to 3,148,907
_RS16670
LU632_RS16675: LU632_RS16675 - hypothetical protein, at 3,148,976 to 3,149,224
_RS16675
LU632_RS16680: LU632_RS16680 - hypothetical protein, at 3,149,221 to 3,149,448
_RS16680
LU632_RS16685: LU632_RS16685 - HNH endonuclease, at 3,149,430 to 3,150,023
_RS16685
Position (kb)
3148
3149 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3147.508 kb on + strand at 3147.576 kb on + strand at 3147.576 kb on + strand at 3147.577 kb on - strand at 3147.580 kb on + strand at 3147.581 kb on - strand at 3147.706 kb on + strand, within LU632_RS16660 at 3147.709 kb on - strand, within LU632_RS16660 at 3147.709 kb on - strand, within LU632_RS16660 at 3147.710 kb on + strand, within LU632_RS16660 at 3147.710 kb on + strand, within LU632_RS16660 at 3147.747 kb on + strand, within LU632_RS16660 at 3147.829 kb on - strand, within LU632_RS16660 at 3147.905 kb on + strand, within LU632_RS16660 at 3147.935 kb on + strand, within LU632_RS16660 at 3147.997 kb on - strand, within LU632_RS16660 at 3148.032 kb on + strand, within LU632_RS16660 at 3148.032 kb on + strand, within LU632_RS16660 at 3148.103 kb on + strand, within LU632_RS16660 at 3148.273 kb on - strand at 3148.310 kb on + strand at 3148.311 kb on - strand at 3148.311 kb on - strand at 3148.523 kb on - strand, within LU632_RS16665 at 3148.613 kb on - strand at 3148.673 kb on - strand at 3148.676 kb on + strand at 3148.676 kb on + strand at 3148.827 kb on + strand, within LU632_RS16670 at 3148.926 kb on + strand at 3148.927 kb on - strand at 3148.993 kb on + strand at 3148.993 kb on + strand at 3148.994 kb on - strand at 3148.995 kb on + strand at 3149.015 kb on - strand, within LU632_RS16675 at 3149.029 kb on + strand, within LU632_RS16675 at 3149.126 kb on + strand, within LU632_RS16675 at 3149.127 kb on - strand, within LU632_RS16675 at 3149.150 kb on + strand, within LU632_RS16675 at 3149.207 kb on - strand at 3149.319 kb on - strand, within LU632_RS16680 at 3149.416 kb on - strand, within LU632_RS16680 at 3149.427 kb on + strand at 3149.427 kb on + strand at 3149.428 kb on - strand at 3149.428 kb on - strand at 3149.446 kb on + strand at 3149.447 kb on - strand at 3149.495 kb on + strand, within LU632_RS16685 at 3149.495 kb on + strand, within LU632_RS16685 at 3149.496 kb on - strand, within LU632_RS16685 at 3149.550 kb on - strand, within LU632_RS16685 at 3149.564 kb on + strand, within LU632_RS16685 at 3149.565 kb on - strand, within LU632_RS16685
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 3,147,508 + -0.5 3,147,576 + +0.2 3,147,576 + +0.6 3,147,577 - -2.3 3,147,580 + +0.1 3,147,581 - +1.0 3,147,706 + LU632_RS16660 0.19 +0.7 3,147,709 - LU632_RS16660 0.19 -1.8 3,147,709 - LU632_RS16660 0.19 +0.5 3,147,710 + LU632_RS16660 0.19 -1.0 3,147,710 + LU632_RS16660 0.19 -0.3 3,147,747 + LU632_RS16660 0.24 -0.2 3,147,829 - LU632_RS16660 0.34 +1.6 3,147,905 + LU632_RS16660 0.44 -1.6 3,147,935 + LU632_RS16660 0.48 -0.0 3,147,997 - LU632_RS16660 0.56 -0.7 3,148,032 + LU632_RS16660 0.60 +1.7 3,148,032 + LU632_RS16660 0.60 -0.6 3,148,103 + LU632_RS16660 0.69 -0.6 3,148,273 - +0.6 3,148,310 + -1.6 3,148,311 - +1.0 3,148,311 - -1.0 3,148,523 - LU632_RS16665 0.76 -1.4 3,148,613 - +0.5 3,148,673 - +2.0 3,148,676 + -0.2 3,148,676 + -0.6 3,148,827 + LU632_RS16670 0.67 -1.6 3,148,926 + +0.2 3,148,927 - +0.5 3,148,993 + -0.4 3,148,993 + -0.5 3,148,994 - +1.6 3,148,995 + -0.0 3,149,015 - LU632_RS16675 0.16 -1.0 3,149,029 + LU632_RS16675 0.21 +1.3 3,149,126 + LU632_RS16675 0.60 -0.3 3,149,127 - LU632_RS16675 0.61 -0.6 3,149,150 + LU632_RS16675 0.70 +0.3 3,149,207 - -0.0 3,149,319 - LU632_RS16680 0.43 +0.6 3,149,416 - LU632_RS16680 0.86 -0.0 3,149,427 + +0.6 3,149,427 + -0.0 3,149,428 - +0.7 3,149,428 - -0.5 3,149,446 + -0.8 3,149,447 - +0.2 3,149,495 + LU632_RS16685 0.11 +1.2 3,149,495 + LU632_RS16685 0.11 +0.3 3,149,496 - LU632_RS16685 0.11 -0.2 3,149,550 - LU632_RS16685 0.20 -0.2 3,149,564 + LU632_RS16685 0.23 -2.8 3,149,565 - LU632_RS16685 0.23 -0.4
Or see this region's nucleotide sequence