Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS12865 and oppC are separated by 10 nucleotides oppC and oppB are separated by 13 nucleotides
LU632_RS12865: LU632_RS12865 - ABC transporter ATP-binding protein, at 2,448,857 to 2,449,870
_RS12865
LU632_RS12870: oppC - oligopeptide ABC transporter permease OppC, at 2,449,881 to 2,450,789
oppC
LU632_RS12875: oppB - oligopeptide ABC transporter permease OppB, at 2,450,803 to 2,451,723
oppB
Position (kb)
2449
2450
2451 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2448.908 kb on - strand at 2448.993 kb on - strand, within LU632_RS12865 at 2448.993 kb on - strand, within LU632_RS12865 at 2449.000 kb on + strand, within LU632_RS12865 at 2449.018 kb on + strand, within LU632_RS12865 at 2449.019 kb on - strand, within LU632_RS12865 at 2449.104 kb on - strand, within LU632_RS12865 at 2449.104 kb on - strand, within LU632_RS12865 at 2449.136 kb on - strand, within LU632_RS12865 at 2449.143 kb on + strand, within LU632_RS12865 at 2449.143 kb on + strand, within LU632_RS12865 at 2449.143 kb on + strand, within LU632_RS12865 at 2449.143 kb on + strand, within LU632_RS12865 at 2449.143 kb on + strand, within LU632_RS12865 at 2449.143 kb on + strand, within LU632_RS12865 at 2449.144 kb on - strand, within LU632_RS12865 at 2449.144 kb on - strand, within LU632_RS12865 at 2449.202 kb on - strand, within LU632_RS12865 at 2449.216 kb on + strand, within LU632_RS12865 at 2449.216 kb on + strand, within LU632_RS12865 at 2449.228 kb on + strand, within LU632_RS12865 at 2449.249 kb on + strand, within LU632_RS12865 at 2449.250 kb on - strand, within LU632_RS12865 at 2449.268 kb on + strand, within LU632_RS12865 at 2449.282 kb on + strand, within LU632_RS12865 at 2449.340 kb on + strand, within LU632_RS12865 at 2449.490 kb on + strand, within LU632_RS12865 at 2449.510 kb on + strand, within LU632_RS12865 at 2449.510 kb on + strand, within LU632_RS12865 at 2449.615 kb on + strand, within LU632_RS12865 at 2449.616 kb on - strand, within LU632_RS12865 at 2449.716 kb on - strand, within LU632_RS12865 at 2449.784 kb on - strand at 2449.926 kb on - strand at 2449.974 kb on + strand, within oppC at 2449.974 kb on + strand, within oppC at 2449.974 kb on + strand, within oppC at 2450.116 kb on + strand, within oppC at 2450.127 kb on + strand, within oppC at 2450.150 kb on + strand, within oppC at 2450.279 kb on + strand, within oppC at 2450.303 kb on + strand, within oppC at 2450.343 kb on - strand, within oppC at 2450.511 kb on + strand, within oppC at 2450.511 kb on + strand, within oppC at 2450.599 kb on + strand, within oppC at 2450.603 kb on + strand, within oppC at 2450.604 kb on - strand, within oppC at 2450.684 kb on + strand, within oppC at 2450.688 kb on + strand, within oppC at 2450.689 kb on - strand, within oppC at 2450.689 kb on - strand, within oppC at 2450.689 kb on - strand, within oppC at 2450.689 kb on - strand, within oppC at 2450.938 kb on + strand, within oppB at 2450.939 kb on - strand, within oppB at 2450.977 kb on + strand, within oppB at 2451.020 kb on + strand, within oppB at 2451.125 kb on - strand, within oppB at 2451.170 kb on + strand, within oppB at 2451.171 kb on - strand, within oppB at 2451.348 kb on + strand, within oppB at 2451.349 kb on - strand, within oppB at 2451.349 kb on - strand, within oppB at 2451.349 kb on - strand, within oppB at 2451.349 kb on - strand, within oppB at 2451.349 kb on - strand, within oppB at 2451.349 kb on - strand, within oppB at 2451.392 kb on - strand, within oppB at 2451.455 kb on - strand, within oppB at 2451.481 kb on - strand, within oppB at 2451.482 kb on + strand, within oppB at 2451.482 kb on + strand, within oppB at 2451.521 kb on - strand, within oppB at 2451.521 kb on - strand, within oppB at 2451.527 kb on + strand, within oppB at 2451.559 kb on - strand, within oppB at 2451.559 kb on - strand, within oppB at 2451.567 kb on - strand, within oppB at 2451.618 kb on + strand, within oppB at 2451.660 kb on + strand at 2451.660 kb on + strand at 2451.661 kb on - strand at 2451.664 kb on - strand at 2451.707 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 2,448,908 - -1.4 2,448,993 - LU632_RS12865 0.13 -1.4 2,448,993 - LU632_RS12865 0.13 +1.5 2,449,000 + LU632_RS12865 0.14 -0.9 2,449,018 + LU632_RS12865 0.16 -1.5 2,449,019 - LU632_RS12865 0.16 -0.1 2,449,104 - LU632_RS12865 0.24 -0.1 2,449,104 - LU632_RS12865 0.24 -1.1 2,449,136 - LU632_RS12865 0.28 -1.9 2,449,143 + LU632_RS12865 0.28 +0.9 2,449,143 + LU632_RS12865 0.28 -1.5 2,449,143 + LU632_RS12865 0.28 -1.8 2,449,143 + LU632_RS12865 0.28 -1.9 2,449,143 + LU632_RS12865 0.28 +0.7 2,449,143 + LU632_RS12865 0.28 -1.5 2,449,144 - LU632_RS12865 0.28 -0.1 2,449,144 - LU632_RS12865 0.28 -1.4 2,449,202 - LU632_RS12865 0.34 -0.7 2,449,216 + LU632_RS12865 0.35 +0.9 2,449,216 + LU632_RS12865 0.35 -0.1 2,449,228 + LU632_RS12865 0.37 -1.7 2,449,249 + LU632_RS12865 0.39 -1.7 2,449,250 - LU632_RS12865 0.39 -1.3 2,449,268 + LU632_RS12865 0.41 -0.1 2,449,282 + LU632_RS12865 0.42 -0.7 2,449,340 + LU632_RS12865 0.48 -0.3 2,449,490 + LU632_RS12865 0.62 -1.9 2,449,510 + LU632_RS12865 0.64 -1.9 2,449,510 + LU632_RS12865 0.64 +0.6 2,449,615 + LU632_RS12865 0.75 -1.1 2,449,616 - LU632_RS12865 0.75 -1.4 2,449,716 - LU632_RS12865 0.85 -0.1 2,449,784 - +0.3 2,449,926 - -0.1 2,449,974 + oppC LU632_RS12870 0.10 -2.4 2,449,974 + oppC LU632_RS12870 0.10 -1.9 2,449,974 + oppC LU632_RS12870 0.10 +0.3 2,450,116 + oppC LU632_RS12870 0.26 -1.8 2,450,127 + oppC LU632_RS12870 0.27 -1.7 2,450,150 + oppC LU632_RS12870 0.30 -0.1 2,450,279 + oppC LU632_RS12870 0.44 -2.0 2,450,303 + oppC LU632_RS12870 0.46 -0.7 2,450,343 - oppC LU632_RS12870 0.51 -1.7 2,450,511 + oppC LU632_RS12870 0.69 -0.7 2,450,511 + oppC LU632_RS12870 0.69 -0.1 2,450,599 + oppC LU632_RS12870 0.79 -0.4 2,450,603 + oppC LU632_RS12870 0.79 -1.7 2,450,604 - oppC LU632_RS12870 0.80 -2.1 2,450,684 + oppC LU632_RS12870 0.88 -0.1 2,450,688 + oppC LU632_RS12870 0.89 -0.6 2,450,689 - oppC LU632_RS12870 0.89 -1.1 2,450,689 - oppC LU632_RS12870 0.89 +1.5 2,450,689 - oppC LU632_RS12870 0.89 -1.4 2,450,689 - oppC LU632_RS12870 0.89 +0.2 2,450,938 + oppB LU632_RS12875 0.15 -0.8 2,450,939 - oppB LU632_RS12875 0.15 -0.1 2,450,977 + oppB LU632_RS12875 0.19 -0.7 2,451,020 + oppB LU632_RS12875 0.24 -1.8 2,451,125 - oppB LU632_RS12875 0.35 -0.7 2,451,170 + oppB LU632_RS12875 0.40 +0.3 2,451,171 - oppB LU632_RS12875 0.40 -0.7 2,451,348 + oppB LU632_RS12875 0.59 -1.2 2,451,349 - oppB LU632_RS12875 0.59 -2.4 2,451,349 - oppB LU632_RS12875 0.59 -1.5 2,451,349 - oppB LU632_RS12875 0.59 -1.7 2,451,349 - oppB LU632_RS12875 0.59 -1.3 2,451,349 - oppB LU632_RS12875 0.59 -1.1 2,451,349 - oppB LU632_RS12875 0.59 -0.1 2,451,392 - oppB LU632_RS12875 0.64 -2.2 2,451,455 - oppB LU632_RS12875 0.71 -1.6 2,451,481 - oppB LU632_RS12875 0.74 +1.2 2,451,482 + oppB LU632_RS12875 0.74 +0.9 2,451,482 + oppB LU632_RS12875 0.74 +1.9 2,451,521 - oppB LU632_RS12875 0.78 -1.1 2,451,521 - oppB LU632_RS12875 0.78 -3.2 2,451,527 + oppB LU632_RS12875 0.79 -1.7 2,451,559 - oppB LU632_RS12875 0.82 -1.9 2,451,559 - oppB LU632_RS12875 0.82 +0.2 2,451,567 - oppB LU632_RS12875 0.83 -2.0 2,451,618 + oppB LU632_RS12875 0.88 -0.1 2,451,660 + -1.4 2,451,660 + -2.0 2,451,661 - -0.8 2,451,664 - -3.1 2,451,707 - -2.4
Or see this region's nucleotide sequence