Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppsA and ydiK are separated by 413 nucleotides ydiK and LU632_RS11040 are separated by 220 nucleotides
LU632_RS11025: ppsA - phosphoenolpyruvate synthase, at 2,107,852 to 2,110,236
ppsA
LU632_RS11035: ydiK - AI-2E family transporter YdiK, at 2,110,650 to 2,111,765
ydiK
LU632_RS11040: LU632_RS11040 - FAD-binding oxidoreductase, at 2,111,986 to 2,115,045
_RS11040
Position (kb)
2110
2111
2112 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2109.738 kb on - strand, within ppsA at 2109.795 kb on - strand, within ppsA at 2109.821 kb on + strand, within ppsA at 2109.879 kb on + strand, within ppsA at 2109.880 kb on - strand, within ppsA at 2109.880 kb on - strand, within ppsA at 2109.880 kb on - strand, within ppsA at 2110.052 kb on + strand at 2110.053 kb on - strand at 2110.056 kb on + strand at 2110.057 kb on - strand at 2110.086 kb on + strand at 2110.090 kb on + strand at 2110.242 kb on + strand at 2110.247 kb on - strand at 2110.392 kb on - strand at 2110.451 kb on - strand at 2110.500 kb on + strand at 2110.503 kb on + strand at 2110.625 kb on + strand at 2110.625 kb on + strand at 2110.652 kb on - strand at 2110.745 kb on + strand at 2110.917 kb on + strand, within ydiK at 2111.019 kb on + strand, within ydiK at 2111.064 kb on - strand, within ydiK at 2111.064 kb on - strand, within ydiK at 2111.253 kb on + strand, within ydiK at 2111.254 kb on - strand, within ydiK at 2111.381 kb on + strand, within ydiK at 2111.381 kb on + strand, within ydiK at 2111.381 kb on + strand, within ydiK at 2111.382 kb on - strand, within ydiK at 2111.382 kb on - strand, within ydiK at 2111.382 kb on - strand, within ydiK at 2111.517 kb on - strand, within ydiK at 2111.532 kb on - strand, within ydiK at 2111.536 kb on - strand, within ydiK at 2111.542 kb on - strand, within ydiK at 2111.640 kb on - strand, within ydiK at 2111.640 kb on - strand, within ydiK at 2111.773 kb on + strand at 2111.774 kb on - strand at 2111.777 kb on + strand at 2111.777 kb on + strand at 2111.778 kb on - strand at 2111.786 kb on + strand at 2111.869 kb on - strand at 2111.896 kb on - strand at 2111.961 kb on + strand at 2112.123 kb on + strand at 2112.123 kb on + strand at 2112.124 kb on - strand at 2112.124 kb on - strand at 2112.176 kb on + strand at 2112.176 kb on + strand at 2112.176 kb on + strand at 2112.176 kb on + strand at 2112.176 kb on + strand at 2112.176 kb on + strand at 2112.177 kb on - strand at 2112.177 kb on - strand at 2112.292 kb on - strand, within LU632_RS11040 at 2112.315 kb on + strand, within LU632_RS11040 at 2112.316 kb on - strand, within LU632_RS11040 at 2112.316 kb on - strand, within LU632_RS11040 at 2112.321 kb on + strand, within LU632_RS11040 at 2112.322 kb on - strand, within LU632_RS11040 at 2112.322 kb on - strand, within LU632_RS11040
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 2,109,738 - ppsA LU632_RS11025 0.79 -0.3 2,109,795 - ppsA LU632_RS11025 0.81 +1.1 2,109,821 + ppsA LU632_RS11025 0.83 -0.2 2,109,879 + ppsA LU632_RS11025 0.85 +0.4 2,109,880 - ppsA LU632_RS11025 0.85 -1.1 2,109,880 - ppsA LU632_RS11025 0.85 -1.1 2,109,880 - ppsA LU632_RS11025 0.85 -1.1 2,110,052 + -1.1 2,110,053 - -0.1 2,110,056 + -1.7 2,110,057 - +0.8 2,110,086 + -0.1 2,110,090 + -0.5 2,110,242 + -0.2 2,110,247 - -0.5 2,110,392 - +0.8 2,110,451 - -0.2 2,110,500 + +0.0 2,110,503 + +1.5 2,110,625 + +0.3 2,110,625 + -0.1 2,110,652 - -1.1 2,110,745 + -1.7 2,110,917 + ydiK LU632_RS11035 0.24 +0.9 2,111,019 + ydiK LU632_RS11035 0.33 -0.1 2,111,064 - ydiK LU632_RS11035 0.37 -0.1 2,111,064 - ydiK LU632_RS11035 0.37 -1.1 2,111,253 + ydiK LU632_RS11035 0.54 -1.1 2,111,254 - ydiK LU632_RS11035 0.54 -1.3 2,111,381 + ydiK LU632_RS11035 0.66 -1.7 2,111,381 + ydiK LU632_RS11035 0.66 -1.1 2,111,381 + ydiK LU632_RS11035 0.66 -1.7 2,111,382 - ydiK LU632_RS11035 0.66 -1.4 2,111,382 - ydiK LU632_RS11035 0.66 -0.1 2,111,382 - ydiK LU632_RS11035 0.66 -0.1 2,111,517 - ydiK LU632_RS11035 0.78 -1.7 2,111,532 - ydiK LU632_RS11035 0.79 +1.2 2,111,536 - ydiK LU632_RS11035 0.79 -0.1 2,111,542 - ydiK LU632_RS11035 0.80 +0.9 2,111,640 - ydiK LU632_RS11035 0.89 -1.1 2,111,640 - ydiK LU632_RS11035 0.89 -0.5 2,111,773 + -0.1 2,111,774 - -0.1 2,111,777 + -1.4 2,111,777 + -1.7 2,111,778 - -0.1 2,111,786 + -0.6 2,111,869 - +0.9 2,111,896 - -0.1 2,111,961 + -2.1 2,112,123 + -0.1 2,112,123 + -0.1 2,112,124 - -0.7 2,112,124 - -0.1 2,112,176 + -1.9 2,112,176 + +0.4 2,112,176 + -0.8 2,112,176 + +0.1 2,112,176 + -1.7 2,112,176 + -2.7 2,112,177 - -2.3 2,112,177 - -0.1 2,112,292 - LU632_RS11040 0.10 -1.1 2,112,315 + LU632_RS11040 0.11 -0.7 2,112,316 - LU632_RS11040 0.11 -2.3 2,112,316 - LU632_RS11040 0.11 -3.3 2,112,321 + LU632_RS11040 0.11 -0.6 2,112,322 - LU632_RS11040 0.11 -0.8 2,112,322 - LU632_RS11040 0.11 -1.7
Or see this region's nucleotide sequence