Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt polB and LU632_RS02815 are separated by 77 nucleotides LU632_RS02815 and LU632_RS02820 are separated by 158 nucleotides LU632_RS02820 and thiQ are separated by 232 nucleotides thiQ and thiP overlap by 17 nucleotides
LU632_RS02810: polB - DNA polymerase II, at 570,626 to 572,986
polB
LU632_RS02815: LU632_RS02815 - transposase, at 573,064 to 573,488
_RS02815
LU632_RS02820: LU632_RS02820 - DedA family protein, at 573,647 to 574,414
_RS02820
LU632_RS02825: thiQ - thiamine ABC transporter ATP-binding protein ThiQ, at 574,647 to 575,348
thiQ
LU632_RS02830: thiP - thiamine/thiamine pyrophosphate ABC transporter permease ThiP, at 575,332 to 576,942
thiP
Position (kb) 573
574
575 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 572.670 kb on + strand, within polB at 572.671 kb on - strand, within polB at 572.671 kb on - strand, within polB at 572.678 kb on + strand, within polB at 573.021 kb on + strand at 573.021 kb on + strand at 573.021 kb on + strand at 573.021 kb on + strand at 573.022 kb on - strand at 573.228 kb on - strand, within LU632_RS02815 at 573.228 kb on - strand, within LU632_RS02815 at 573.445 kb on + strand, within LU632_RS02815 at 573.472 kb on - strand at 573.476 kb on + strand at 573.517 kb on + strand at 573.518 kb on - strand at 573.521 kb on + strand at 573.521 kb on + strand at 573.521 kb on + strand at 573.521 kb on + strand at 573.522 kb on - strand at 573.523 kb on + strand at 573.552 kb on + strand at 573.553 kb on - strand at 573.553 kb on - strand at 573.647 kb on - strand at 573.647 kb on - strand at 573.670 kb on + strand at 573.670 kb on + strand at 573.671 kb on - strand at 573.671 kb on - strand at 573.671 kb on - strand at 573.671 kb on - strand at 573.672 kb on + strand at 573.672 kb on + strand at 573.916 kb on + strand, within LU632_RS02820 at 573.975 kb on + strand, within LU632_RS02820 at 573.975 kb on + strand, within LU632_RS02820 at 573.975 kb on + strand, within LU632_RS02820 at 574.093 kb on - strand, within LU632_RS02820 at 574.160 kb on - strand, within LU632_RS02820 at 574.165 kb on + strand, within LU632_RS02820 at 574.172 kb on - strand, within LU632_RS02820 at 574.177 kb on + strand, within LU632_RS02820 at 574.177 kb on + strand, within LU632_RS02820 at 574.177 kb on + strand, within LU632_RS02820 at 574.178 kb on - strand, within LU632_RS02820 at 574.178 kb on - strand, within LU632_RS02820 at 574.178 kb on - strand, within LU632_RS02820 at 574.343 kb on - strand at 574.386 kb on + strand at 574.386 kb on + strand at 574.386 kb on + strand at 574.412 kb on + strand at 574.489 kb on - strand at 574.493 kb on - strand at 574.494 kb on - strand at 574.649 kb on - strand at 574.748 kb on + strand, within thiQ at 574.748 kb on + strand, within thiQ at 574.748 kb on + strand, within thiQ at 574.791 kb on + strand, within thiQ at 574.808 kb on + strand, within thiQ at 574.808 kb on + strand, within thiQ at 574.808 kb on + strand, within thiQ at 574.809 kb on - strand, within thiQ at 574.809 kb on - strand, within thiQ at 574.824 kb on + strand, within thiQ at 574.824 kb on + strand, within thiQ at 574.825 kb on - strand, within thiQ at 574.825 kb on - strand, within thiQ at 575.013 kb on - strand, within thiQ at 575.091 kb on + strand, within thiQ at 575.283 kb on + strand at 575.284 kb on - strand at 575.284 kb on - strand at 575.317 kb on + strand at 575.333 kb on + strand at 575.343 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 572,670 + polB LU632_RS02810 0.87 -0.2 572,671 - polB LU632_RS02810 0.87 +0.4 572,671 - polB LU632_RS02810 0.87 +0.0 572,678 + polB LU632_RS02810 0.87 +0.0 573,021 + -0.4 573,021 + -0.4 573,021 + +0.8 573,021 + +0.0 573,022 - +1.0 573,228 - LU632_RS02815 0.39 -1.8 573,228 - LU632_RS02815 0.39 +1.0 573,445 + LU632_RS02815 0.90 +1.0 573,472 - -0.4 573,476 + -0.2 573,517 + +0.6 573,518 - +0.2 573,521 + -0.6 573,521 + +0.6 573,521 + +0.3 573,521 + +0.6 573,522 - +0.3 573,523 + +0.0 573,552 + -1.0 573,553 - -1.1 573,553 - -1.3 573,647 - -0.8 573,647 - -1.8 573,670 + -1.8 573,670 + -0.8 573,671 - -1.4 573,671 - -2.3 573,671 - -0.6 573,671 - -0.4 573,672 + -2.0 573,672 + -1.0 573,916 + LU632_RS02820 0.35 -2.0 573,975 + LU632_RS02820 0.43 -3.0 573,975 + LU632_RS02820 0.43 -0.7 573,975 + LU632_RS02820 0.43 -0.4 574,093 - LU632_RS02820 0.58 +0.0 574,160 - LU632_RS02820 0.67 -1.3 574,165 + LU632_RS02820 0.67 +0.0 574,172 - LU632_RS02820 0.68 -1.2 574,177 + LU632_RS02820 0.69 -0.5 574,177 + LU632_RS02820 0.69 -2.3 574,177 + LU632_RS02820 0.69 +0.0 574,178 - LU632_RS02820 0.69 +1.3 574,178 - LU632_RS02820 0.69 -1.8 574,178 - LU632_RS02820 0.69 +0.6 574,343 - -1.3 574,386 + -0.9 574,386 + -1.6 574,386 + +0.0 574,412 + -1.8 574,489 - -0.8 574,493 - -0.1 574,494 - -1.3 574,649 - +0.4 574,748 + thiQ LU632_RS02825 0.14 +1.6 574,748 + thiQ LU632_RS02825 0.14 +0.5 574,748 + thiQ LU632_RS02825 0.14 -0.6 574,791 + thiQ LU632_RS02825 0.21 +1.0 574,808 + thiQ LU632_RS02825 0.23 -0.1 574,808 + thiQ LU632_RS02825 0.23 -2.0 574,808 + thiQ LU632_RS02825 0.23 -0.6 574,809 - thiQ LU632_RS02825 0.23 +0.1 574,809 - thiQ LU632_RS02825 0.23 -1.6 574,824 + thiQ LU632_RS02825 0.25 -0.3 574,824 + thiQ LU632_RS02825 0.25 -0.7 574,825 - thiQ LU632_RS02825 0.25 +0.2 574,825 - thiQ LU632_RS02825 0.25 -1.0 575,013 - thiQ LU632_RS02825 0.52 -0.4 575,091 + thiQ LU632_RS02825 0.63 +0.0 575,283 + -0.7 575,284 - +2.0 575,284 - -0.6 575,317 + +1.3 575,333 + -0.2 575,343 + -0.4
Or see this region's nucleotide sequence