Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS01895 and blc are separated by 312 nucleotides blc and frdD are separated by 76 nucleotides frdD and frdC overlap by 4 nucleotides frdC and LU632_RS01915 are separated by 11 nucleotides
LU632_RS01895: LU632_RS01895 - ECN family pore-forming entericidin, at 363,215 to 363,349
_RS01895
LU632_RS01900: blc - outer membrane lipoprotein Blc, at 363,662 to 364,186
blc
LU632_RS01905: frdD - fumarate reductase subunit FrdD, at 364,263 to 364,616
frdD
LU632_RS01910: frdC - fumarate reductase subunit FrdC, at 364,613 to 365,017
frdC
LU632_RS01915: LU632_RS01915 - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 365,029 to 365,763
_RS01915
Position (kb) 364
365 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 363.312 kb on + strand, within LU632_RS01895 at 363.312 kb on + strand, within LU632_RS01895 at 363.313 kb on - strand, within LU632_RS01895 at 363.348 kb on - strand at 363.350 kb on + strand at 363.353 kb on + strand at 363.366 kb on + strand at 363.463 kb on + strand at 363.465 kb on + strand at 363.465 kb on + strand at 363.598 kb on + strand at 363.664 kb on - strand at 363.664 kb on - strand at 363.736 kb on + strand, within blc at 363.739 kb on + strand, within blc at 363.739 kb on + strand, within blc at 363.740 kb on - strand, within blc at 363.785 kb on - strand, within blc at 363.852 kb on + strand, within blc at 363.909 kb on + strand, within blc at 363.952 kb on + strand, within blc at 363.952 kb on + strand, within blc at 363.952 kb on + strand, within blc at 363.952 kb on + strand, within blc at 363.952 kb on + strand, within blc at 363.952 kb on + strand, within blc at 363.953 kb on - strand, within blc at 363.953 kb on - strand, within blc at 363.953 kb on - strand, within blc at 364.074 kb on + strand, within blc at 364.075 kb on - strand, within blc at 364.107 kb on - strand, within blc at 364.123 kb on + strand, within blc at 364.123 kb on + strand, within blc at 364.123 kb on + strand, within blc at 364.186 kb on + strand at 364.186 kb on + strand at 364.186 kb on + strand at 364.187 kb on - strand at 364.187 kb on - strand at 364.187 kb on - strand at 364.187 kb on - strand at 364.187 kb on - strand at 364.187 kb on - strand at 364.187 kb on - strand at 364.187 kb on - strand at 364.187 kb on - strand at 364.187 kb on - strand at 364.199 kb on + strand at 364.219 kb on + strand at 364.221 kb on + strand at 364.221 kb on + strand at 364.265 kb on - strand at 364.284 kb on + strand at 364.285 kb on - strand at 364.285 kb on - strand at 364.352 kb on + strand, within frdD at 364.468 kb on + strand, within frdD at 364.468 kb on + strand, within frdD at 364.493 kb on + strand, within frdD at 364.494 kb on - strand, within frdD at 364.506 kb on + strand, within frdD at 364.616 kb on + strand at 364.726 kb on + strand, within frdC at 364.727 kb on - strand, within frdC at 364.768 kb on + strand, within frdC at 364.769 kb on - strand, within frdC at 364.798 kb on + strand, within frdC at 364.799 kb on - strand, within frdC at 364.812 kb on - strand, within frdC at 364.939 kb on + strand, within frdC at 364.940 kb on - strand, within frdC at 364.941 kb on + strand, within frdC at 364.941 kb on + strand, within frdC at 364.942 kb on - strand, within frdC at 364.996 kb on - strand at 365.135 kb on + strand, within LU632_RS01915 at 365.148 kb on + strand, within LU632_RS01915 at 365.224 kb on - strand, within LU632_RS01915 at 365.336 kb on + strand, within LU632_RS01915 at 365.337 kb on - strand, within LU632_RS01915 at 365.405 kb on + strand, within LU632_RS01915 at 365.405 kb on + strand, within LU632_RS01915 at 365.405 kb on + strand, within LU632_RS01915 at 365.406 kb on - strand, within LU632_RS01915 at 365.406 kb on - strand, within LU632_RS01915 at 365.615 kb on + strand, within LU632_RS01915 at 365.615 kb on + strand, within LU632_RS01915 at 365.616 kb on - strand, within LU632_RS01915
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 363,312 + LU632_RS01895 0.72 -0.1 363,312 + LU632_RS01895 0.72 -2.0 363,313 - LU632_RS01895 0.73 -0.2 363,348 - -0.2 363,350 + +0.0 363,353 + +0.1 363,366 + -0.5 363,463 + +0.3 363,465 + -0.1 363,465 + +0.0 363,598 + -0.4 363,664 - -0.2 363,664 - +0.4 363,736 + blc LU632_RS01900 0.14 +0.5 363,739 + blc LU632_RS01900 0.15 -0.1 363,739 + blc LU632_RS01900 0.15 +0.5 363,740 - blc LU632_RS01900 0.15 +0.5 363,785 - blc LU632_RS01900 0.23 -0.6 363,852 + blc LU632_RS01900 0.36 -0.7 363,909 + blc LU632_RS01900 0.47 +0.6 363,952 + blc LU632_RS01900 0.55 +1.2 363,952 + blc LU632_RS01900 0.55 +0.5 363,952 + blc LU632_RS01900 0.55 -0.3 363,952 + blc LU632_RS01900 0.55 +0.6 363,952 + blc LU632_RS01900 0.55 +0.0 363,952 + blc LU632_RS01900 0.55 -2.6 363,953 - blc LU632_RS01900 0.55 +0.2 363,953 - blc LU632_RS01900 0.55 +1.3 363,953 - blc LU632_RS01900 0.55 -0.1 364,074 + blc LU632_RS01900 0.78 -1.0 364,075 - blc LU632_RS01900 0.79 +0.0 364,107 - blc LU632_RS01900 0.85 +0.5 364,123 + blc LU632_RS01900 0.88 +0.5 364,123 + blc LU632_RS01900 0.88 +3.2 364,123 + blc LU632_RS01900 0.88 +1.0 364,186 + -0.4 364,186 + +1.5 364,186 + +0.0 364,187 - -0.7 364,187 - -0.4 364,187 - +0.4 364,187 - +0.8 364,187 - +0.0 364,187 - -0.9 364,187 - -0.2 364,187 - -0.9 364,187 - -0.1 364,187 - -2.2 364,199 + +0.8 364,219 + -0.2 364,221 + +0.6 364,221 + +0.6 364,265 - -1.6 364,284 + -0.2 364,285 - +0.2 364,285 - +1.0 364,352 + frdD LU632_RS01905 0.25 +0.6 364,468 + frdD LU632_RS01905 0.58 -0.4 364,468 + frdD LU632_RS01905 0.58 -0.7 364,493 + frdD LU632_RS01905 0.65 +0.5 364,494 - frdD LU632_RS01905 0.65 +0.8 364,506 + frdD LU632_RS01905 0.69 +1.3 364,616 + -0.2 364,726 + frdC LU632_RS01910 0.28 -1.2 364,727 - frdC LU632_RS01910 0.28 -0.8 364,768 + frdC LU632_RS01910 0.38 -0.4 364,769 - frdC LU632_RS01910 0.39 -0.7 364,798 + frdC LU632_RS01910 0.46 +0.8 364,799 - frdC LU632_RS01910 0.46 -0.2 364,812 - frdC LU632_RS01910 0.49 +1.0 364,939 + frdC LU632_RS01910 0.80 +0.2 364,940 - frdC LU632_RS01910 0.81 -0.2 364,941 + frdC LU632_RS01910 0.81 +1.2 364,941 + frdC LU632_RS01910 0.81 -1.6 364,942 - frdC LU632_RS01910 0.81 -0.3 364,996 - -0.2 365,135 + LU632_RS01915 0.14 +0.0 365,148 + LU632_RS01915 0.16 +0.4 365,224 - LU632_RS01915 0.27 +0.0 365,336 + LU632_RS01915 0.42 -0.7 365,337 - LU632_RS01915 0.42 +1.0 365,405 + LU632_RS01915 0.51 -0.0 365,405 + LU632_RS01915 0.51 +0.8 365,405 + LU632_RS01915 0.51 +0.3 365,406 - LU632_RS01915 0.51 +0.6 365,406 - LU632_RS01915 0.51 +0.0 365,615 + LU632_RS01915 0.80 +0.8 365,615 + LU632_RS01915 0.80 +0.0 365,616 - LU632_RS01915 0.80 +1.6
Or see this region's nucleotide sequence